Restriction map for
pF1KB6674
(Linear) (Six-base) MAPSORT of: KIBB6674.seq Check: 9203 from: 1 to: 1362
KIBB6674.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 785 1362
Size: 785 577
AlwNI CAG_nnn'CTG
Cuts at: 0 84 603 1362
Size: 84 519 759
Fragments arranged by size:
759 519 84
ApaI G_GGCC'C
Cuts at: 0 1201 1271 1362
Size: 1201 70 91
Fragments arranged by size:
1201 91 70
ApoI r'AATT_y
Cuts at: 0 1066 1362
Size: 1066 296
AvaI C'yCGr_G
Cuts at: 0 300 556 1362
Size: 300 256 806
Fragments arranged by size:
806 300 256
BanII G_rGCy'C
Cuts at: 0 307 596 1201 1271 1362
Size: 307 289 605 70 91
Fragments arranged by size:
605 307 289 91 70
BbvCI CC'TCA_GC
Cuts at: 0 986 1362
Size: 986 376
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 709 743 1362
Size: 709 34 619
Fragments arranged by size:
709 619 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1020 1362
Size: 1020 342
BclI T'GATC_A
Cuts at: 0 410 1362
Size: 410 952
BglI GCCn_nnn'nGGC
Cuts at: 0 251 1275 1362
Size: 251 1024 87
Fragments arranged by size:
1024 251 87
BlpI GC'TnA_GC
Cuts at: 0 211 1362
Size: 211 1151
Bme1580I G_kGCm'C
Cuts at: 0 1201 1271 1362
Size: 1201 70 91
Fragments arranged by size:
1201 91 70
BmrI ACTGGGnnnn_n'
Cuts at: 0 581 753 867 1195 1203 1362
Size: 581 172 114 328 8 159
Fragments arranged by size:
581 328 172 159 114 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1113 1362
Size: 1113 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 423 502 580 1148 1362
Size: 145 278 79 78 568 214
Fragments arranged by size:
568 278 214 145 79 78
Bpu10I CC'TnA_GC
Cuts at: 0 986 1362
Size: 986 376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1362
Size: 382 980
BsaHI Gr'CG_yC
Cuts at: 0 800 1362
Size: 800 562
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 475 505 1362
Size: 475 30 857
Fragments arranged by size:
857 475 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 947 1362
Size: 37 910 415
Fragments arranged by size:
910 415 37
BseYI C'CCAG_C
Cuts at: 0 188 238 249 1362
Size: 188 50 11 1113
Fragments arranged by size:
1113 188 50 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 1362
Size: 244 1118
BsiHKAI G_wGCw'C
Cuts at: 0 155 596 920 1362
Size: 155 441 324 442
Fragments arranged by size:
442 441 324 155
Bsp1286I G_dGCh'C
Cuts at: 0 155 307 596 920 1201 1271 1362
Size: 155 152 289 324 281 70 91
Fragments arranged by size:
324 289 281 155 152 91 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 402 785 1362
Size: 402 383 577
Fragments arranged by size:
577 402 383
BsrBI CCG'CTC
Cuts at: 0 486 1362
Size: 486 876
BsrGI T'GTAC_A
Cuts at: 0 137 1362
Size: 137 1225
BssSI C'ACGA_G
Cuts at: 0 341 1362
Size: 341 1021
BstYI r'GATC_y
Cuts at: 0 432 1362
Size: 432 930
BtgI C'CryG_G
Cuts at: 0 844 1182 1362
Size: 844 338 180
Fragments arranged by size:
844 338 180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 562 1362
Size: 562 800
BtsI GCAGTG_nn'
Cuts at: 0 172 669 1362
Size: 172 497 693
Fragments arranged by size:
693 497 172
DraIII CAC_nnn'GTG
Cuts at: 0 321 1362
Size: 321 1041
EaeI y'GGCC_r
Cuts at: 0 309 659 1362
Size: 309 350 703
Fragments arranged by size:
703 350 309
EarI CTCTTCn'nnn_
Cuts at: 0 371 1012 1362
Size: 371 641 350
Fragments arranged by size:
641 371 350
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 277 1362
Size: 277 1085
EcoICRI GAG'CTC
Cuts at: 0 594 1362
Size: 594 768
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 423 502 580 1148 1362
Size: 145 278 79 78 568 214
Fragments arranged by size:
568 278 214 145 79 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1021 1362
Size: 1021 341
EcoO109I rG'GnC_Cy
Cuts at: 0 144 1197 1267 1268 1362
Size: 144 1053 70 1 94
Fragments arranged by size:
1053 144 94 70 1
EcoRI G'AATT_C
Cuts at: 0 1066 1362
Size: 1066 296
FspI TGC'GCA
Cuts at: 0 499 1362
Size: 499 863
FspAI rTGC'GCAy
Cuts at: 0 499 1362
Size: 499 863
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 261 293 858 890 1362
Size: 261 32 565 32 472
Fragments arranged by size:
565 472 261 32 32
MscI TGG'CCA
Cuts at: 0 311 1362
Size: 311 1051
MslI CAynn'nnrTG
Cuts at: 0 547 731 1362
Size: 547 184 631
Fragments arranged by size:
631 547 184
MspA1I CmG'CkG
Cuts at: 0 39 84 184 192 231 249 1281 1362
Size: 39 45 100 8 39 18 1032 81
Fragments arranged by size:
1032 100 81 45 39 39 18 8
NcoI C'CATG_G
Cuts at: 0 1182 1362
Size: 1182 180
NdeI CA'TA_TG
Cuts at: 0 387 1362
Size: 387 975
NspI r_CATG'y
Cuts at: 0 712 757 1362
Size: 712 45 605
Fragments arranged by size:
712 605 45
PflMI CCAn_nnn'nTGG
Cuts at: 0 234 603 1168 1362
Size: 234 369 565 194
Fragments arranged by size:
565 369 234 194
PpuMI rG'GwC_Cy
Cuts at: 0 144 1362
Size: 144 1218
PspOMI G'GGCC_C
Cuts at: 0 1197 1267 1362
Size: 1197 70 95
Fragments arranged by size:
1197 95 70
PstI C_TGCA'G
Cuts at: 0 1276 1362
Size: 1276 86
PvuII CAG'CTG
Cuts at: 0 84 184 192 231 249 1362
Size: 84 100 8 39 18 1113
Fragments arranged by size:
1113 100 84 39 18 8
SacI G_AGCT'C
Cuts at: 0 596 1362
Size: 596 766
SapI GCTCTTCn'nnn_
Cuts at: 0 371 1362
Size: 371 991
SbfI CC_TGCA'GG
Cuts at: 0 1276 1362
Size: 1276 86
SexAI A'CCwGG_T
Cuts at: 0 146 404 1362
Size: 146 258 958
Fragments arranged by size:
958 258 146
SfcI C'TryA_G
Cuts at: 0 645 1272 1362
Size: 645 627 90
Fragments arranged by size:
645 627 90
SmlI C'TyrA_G
Cuts at: 0 361 1362
Size: 361 1001
SphI G_CATG'C
Cuts at: 0 712 757 1362
Size: 712 45 605
Fragments arranged by size:
712 605 45
StuI AGG'CCT
Cuts at: 0 67 1362
Size: 67 1295
StyI C'CwwG_G
Cuts at: 0 200 1182 1362
Size: 200 982 180
Fragments arranged by size:
982 200 180
TatI w'GTAC_w
Cuts at: 0 137 714 1362
Size: 137 577 648
Fragments arranged by size:
648 577 137
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1148 1180 1362
Size: 1148 32 182
Fragments arranged by size:
1148 182 32
Tth111I GACn'n_nGTC
Cuts at: 0 52 1362
Size: 52 1310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 235 1117 1362
Size: 235 882 245
Fragments arranged by size:
882 245 235
Enzymes that do cut:
AarI AlwNI ApaI ApoI AvaI BanII BbvCI BcgI
BciVI BclI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BsrGI BssSI BstYI BtgI BtgZI
BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI FspAI Hin4I MscI MslI MspA1I
NcoI NdeI NspI PflMI PpuMI PspOMI PstI PvuII
SacI SapI SbfI SexAI SfcI SmlI SphI StuI
StyI TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BfrBI
BglII BmgBI BmtI BsaI BsaAI BsaBI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BssHII
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EagI EcoRV FalI FseI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI TaqII XbaI XhoI XmaI XmnI ZraI