Restriction map for
pF1KB6578
(Linear) (Six-base) MAPSORT of: KIBB6578.seq Check: 187 from: 1 to: 1206
KIBB6578.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 926 1206
Size: 926 280
AatII G_ACGT'C
Cuts at: 0 272 1206
Size: 272 934
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 744 893 1206
Size: 744 149 313
Fragments arranged by size:
744 313 149
AfeI AGC'GCT
Cuts at: 0 802 1206
Size: 802 404
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 314 346 1206
Size: 314 32 860
Fragments arranged by size:
860 314 32
AlwNI CAG_nnn'CTG
Cuts at: 0 84 944 1092 1206
Size: 84 860 148 114
Fragments arranged by size:
860 148 114 84
ApaI G_GGCC'C
Cuts at: 0 47 417 1051 1157 1206
Size: 47 370 634 106 49
Fragments arranged by size:
634 370 106 49 47
ApaLI G'TGCA_C
Cuts at: 0 442 621 1206
Size: 442 179 585
Fragments arranged by size:
585 442 179
AvaI C'yCGr_G
Cuts at: 0 46 1149 1206
Size: 46 1103 57
Fragments arranged by size:
1103 57 46
BamHI G'GATC_C
Cuts at: 0 582 1206
Size: 582 624
BanI G'GyrC_C
Cuts at: 0 158 1206
Size: 158 1048
BanII G_rGCy'C
Cuts at: 0 47 417 634 854 1031 1051 1157 1206
Size: 47 370 217 220 177 20 106 49
Fragments arranged by size:
370 220 217 177 106 49 47 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 969 1206
Size: 969 237
BclI T'GATC_A
Cuts at: 0 990 1206
Size: 990 216
BglI GCCn_nnn'nGGC
Cuts at: 0 494 953 1035 1206
Size: 494 459 82 171
Fragments arranged by size:
494 459 171 82
Bme1580I G_kGCm'C
Cuts at: 0 47 161 417 446 625 1051 1134 1157
Size: 47 114 256 29 179 426 83 23
Cuts at: 1157 1206
Size: 49
Fragments arranged by size:
426 256 179 114 83 49 47 29
23
BmrI ACTGGGnnnn_n'
Cuts at: 0 456 1159 1206
Size: 456 703 47
Fragments arranged by size:
703 456 47
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1128 1206
Size: 1128 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 1206
Size: 505 701
BsaI GGTCTCn'nnnn_
Cuts at: 0 677 1206
Size: 677 529
BsaHI Gr'CG_yC
Cuts at: 0 269 1206
Size: 269 937
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 364 394 1206
Size: 364 30 812
Fragments arranged by size:
812 364 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 205 366 385 421 1206
Size: 193 12 161 19 36 785
Fragments arranged by size:
785 193 161 36 19 12
BseYI C'CCAG_C
Cuts at: 0 1050 1055 1059 1206
Size: 1050 5 4 147
Fragments arranged by size:
1050 147 5 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 806 1206
Size: 332 474 400
Fragments arranged by size:
474 400 332
BsiEI CG_ry'CG
Cuts at: 0 147 1206
Size: 147 1059
BsiHKAI G_wGCw'C
Cuts at: 0 446 625 1127 1206
Size: 446 179 502 79
Fragments arranged by size:
502 446 179 79
Bsp1286I G_dGCh'C
Cuts at: 0 47 161 417 446 625 634 854 1031
Size: 47 114 256 29 179 9 220 177
Cuts at: 1031 1051 1127 1134 1157 1206
Size: 20 76 7 23 49
Fragments arranged by size:
256 220 179 177 114 76 49 47
29 23 20 9 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 926 1071 1206
Size: 926 145 135
Fragments arranged by size:
926 145 135
BsrBI CCG'CTC
Cuts at: 0 411 1206
Size: 411 795
BssHII G'CGCG_C
Cuts at: 0 183 1206
Size: 183 1023
BssSI C'ACGA_G
Cuts at: 0 1024 1206
Size: 1024 182
BstAPI GCAn_nnn'nTGC
Cuts at: 0 944 1206
Size: 944 262
BstXI CCAn_nnnn'nTGG
Cuts at: 0 261 825 1206
Size: 261 564 381
Fragments arranged by size:
564 381 261
BstYI r'GATC_y
Cuts at: 0 582 1206
Size: 582 624
BtsI GCAGTG_nn'
Cuts at: 0 302 945 1206
Size: 302 643 261
Fragments arranged by size:
643 302 261
ClaI AT'CG_AT
Cuts at: 0 701 1206
Size: 701 505
EaeI y'GGCC_r
Cuts at: 0 75 305 1178 1206
Size: 75 230 873 28
Fragments arranged by size:
873 230 75 28
EarI CTCTTCn'nnn_
Cuts at: 0 398 1102 1165 1206
Size: 398 704 63 41
Fragments arranged by size:
704 398 63 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 157 190 280 1206
Size: 157 33 90 926
Fragments arranged by size:
926 157 90 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 744 893 1206
Size: 505 239 149 313
Fragments arranged by size:
505 313 239 149
EcoO109I rG'GnC_Cy
Cuts at: 0 1047 1153 1154 1206
Size: 1047 106 1 52
Fragments arranged by size:
1047 106 52 1
HaeII r_GCGC'y
Cuts at: 0 460 804 1206
Size: 460 344 402
Fragments arranged by size:
460 402 344
MfeI C'AATT_G
Cuts at: 0 730 1206
Size: 730 476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 1206
Size: 575 631
MscI TGG'CCA
Cuts at: 0 307 1206
Size: 307 899
MslI CAynn'nnrTG
Cuts at: 0 512 1206
Size: 512 694
MspA1I CmG'CkG
Cuts at: 0 336 378 438 525 1059 1206
Size: 336 42 60 87 534 147
Fragments arranged by size:
534 336 147 87 60 42
NspI r_CATG'y
Cuts at: 0 501 1206
Size: 501 705
PasI CC'CwG_GG
Cuts at: 0 1032 1206
Size: 1032 174
PshAI GACnn'nnGTC
Cuts at: 0 947 1206
Size: 947 259
PspOMI G'GGCC_C
Cuts at: 0 43 413 1047 1153 1206
Size: 43 370 634 106 53
Fragments arranged by size:
634 370 106 53 43
PstI C_TGCA'G
Cuts at: 0 233 464 1206
Size: 233 231 742
Fragments arranged by size:
742 233 231
PvuII CAG'CTG
Cuts at: 0 525 1059 1206
Size: 525 534 147
Fragments arranged by size:
534 525 147
SapI GCTCTTCn'nnn_
Cuts at: 0 1102 1206
Size: 1102 104
SfcI C'TryA_G
Cuts at: 0 229 460 1206
Size: 229 231 746
Fragments arranged by size:
746 231 229
SmaI CCC'GGG
Cuts at: 0 48 1151 1206
Size: 48 1103 55
Fragments arranged by size:
1103 55 48
SphI G_CATG'C
Cuts at: 0 501 1206
Size: 501 705
SrfI GCCC'GGGC
Cuts at: 0 48 1206
Size: 48 1158
StuI AGG'CCT
Cuts at: 0 781 1206
Size: 781 425
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 574 1051 1206
Size: 574 477 155
Fragments arranged by size:
574 477 155
XmaI C'CCGG_G
Cuts at: 0 46 1149 1206
Size: 46 1103 57
Fragments arranged by size:
1103 57 46
ZraI GAC'GTC
Cuts at: 0 270 1206
Size: 270 936
Enzymes that do cut:
AarI AatII AcuI AfeI AloI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbsI BclI BglI Bme1580I
BmrI BplI BpmI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BssHII BssSI
BstAPI BstXI BstYI BtsI ClaI EaeI EarI EciI
Eco57MI EcoO109I HaeII MfeI MmeI MscI MslI MspA1I
NspI PasI PshAI PspOMI PstI PvuII SapI SfcI
SmaI SphI SrfI StuI TaqII XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BfrBI BglII BlpI BmgBI BmtI
Bpu10I BpuEI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrFI BsrGI BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI CspCI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SspI StyI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmnI