Restriction map for pF1KB6570
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AgeI        AhdI        AocI        
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbsI        BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BpuAI       
BsaBI       BsaHI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FspI        Hin1I       HindIII     HpaI        
KasI        KpnI        MamI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SalI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspI        SwaI        TfiI        
Tsp509I     Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccI          421  GTCTAC               GT^[AC][GT]AC
AflIII       1010  ACACGT               A^C[GA][CT]GT
AlwI          255  GATCC                GGATC(4/5)
AlwNI         471  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       607  AGCGCT               AGC^GCT
ApaI          918  GGGCCC               GGGCC^C
Asp700I       345  GAACAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          562  CCCGAG               C^[CT]CG[GA]G
BalI         1073  TGGCCA               TGG^CCA
BanI          696  GGTGCC               G^G[CT][GA]CC
BbrPI         331  CACGTG               CAC^GTG
BfaI          400  CTAG                 C^TAG
BlpI         1054  GCTGAGC              GC^T[ACGT]AGC
BpmI          309  CTCCAG               CTGGAG(16/14)
Bpu1102I     1054  GCTGAGC              GC^T[ACGT]AGC
BsaAI         331  CACGTG               [CT]AC^GT[GA]
BsaI          658  GAGACC               GGTCTC(1/5)
BsaWI         188  ACCGGA               [AT]^CCGG[AT]
BsgI          127  CTGCAC               GTGCAG(16/14)
BsoBI         562  CCCGAG               C^[CT]CG[GA]G
Bsp1407I       34  TGTACA               T^GTACA
BsrGI          34  TGTACA               T^GTACA
BssHII        703  GCGCGC               G^CGCGC
BstAPI        470  GCAGCGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         254  AGATCC               [GA]^GATC[CT]
CelII        1054  GCTGAGC              GC^T[ACGT]AGC
DraIII        856  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI           85  GAAGAG               CTCTTC(1/4)
Eco47III      607  AGCGCT               AGC^GCT
FseI         1037  GGCCGGCC             GGCCGG^CC
HincII         46  GTCAAC               GT[CT]^[GA]AC
HindII         46  GTCAAC               GT[CT]^[GA]AC
Ksp632I        85  GAAGAG               CTCTTC(1/4)
KspI          229  CCGCGG               CCGC^GG
MaeI          400  CTAG                 C^TAG
MflI          254  AGATCC               [GA]^GATC[CT]
MluNI        1073  TGGCCA               TGG^CCA
MscI         1073  TGGCCA               TGG^CCA
MseI          142  TTAA                 T^TAA
NaeI         1038  GCCGGC               GCC^GGC
NcoI         1076  CCATGG               C^CATGG
NgoMIV       1038  GCCGGC               G^CCGGC
NspI          641  GCATGC               [GA]CATG^[CT]
PmaCI         331  CACGTG               CAC^GTG
PmlI          331  CACGTG               CAC^GTG
SacII         229  CCGCGG               CCGC^GG
SapI           85  GAAGAGC              GCTCTTC(1/4)
ScaI           65  AGTACT               AGT^ACT
SexAI         857  ACCTGGT              A^CC[AT]GGT
SphI          641  GCATGC               GCATG^C
SspBI          34  TGTACA               T^GTACA
StuI          239  AGGCCT               AGG^CCT
Tru9I         142  TTAA                 T^TAA
XhoII         254  AGATCC               [GA]^GATC[CT]
XmnI          345  GAACAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Alw44I        328  GTGCAC               G^TGCAC
              862  GTGCAC              
ApaLI         328  GTGCAC               G^TGCAC
              862  GTGCAC              
BanII         462  GGGCTC               G[GA]GC[CT]^C
              918  GGGCCC              
BbvCI         315  CCTCAGC              CCTCAGC(-5/-2)
              377  CCTCAGC             
BsmFI         296  GGGAC                GGGAC(10/14)
              887  GGGAC               
BspMI         796  ACCTGC               ACCTGC(4/8)
             1029  GCAGGT              
BsrFI          42  GCCGGT               [GA]^CCGG[CT]
             1038  GCCGGC              
Cfr10I         42  GCCGGT               [GA]^CCGG[CT]
             1038  GCCGGC              
DpnII         255  GATC                 ^GATC
              933  GATC                
EaeI          204  TGGCCG               [CT]^GGCC[GA]
             1073  TGGCCA              
EcoT14I       893  CCAAGG               C^C[AT][AT]GG
             1076  CCATGG              
HgaI          913  GACGC                GACGC(5/10)
              119  GCGTC               
HinfI         565  GAGTC                G^A[ACGT]TC
              688  GAGTC               
MaeII         332  ACGT                 A^CGT
             1012  ACGT                
MboI          255  GATC                 ^GATC
              933  GATC                
NdeII         255  GATC                 ^GATC
              933  GATC                
PflMI         836  CCATCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1161  CCACCTCCTGG         
PleI          565  GAGTC                GAGTC(4/5)
              688  GAGTC               
PpuMI         296  GGGACCC              [GA]G^G[AT]CC[CT]
             1170  GGGTCCT             
PstI          235  CTGCAG               CTGCA^G
             1027  CTGCAG              
Sau3AI        255  GATC                 ^GATC
              933  GATC                
SfcI          235  CTGCAG               C^T[GA][CT]AG
             1027  CTGCAG              
StyI          893  CCAAGG               C^C[AT][AT]GG
             1076  CCATGG              
TaqI          803  TCGA                 T^CGA
             1000  TCGA                
TthHB8I       803  TCGA                 T^CGA
             1000  TCGA                
Van91I        836  CCATCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1161  CCACCTCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AspHI       
AvaII       BbvI        BcnI        BmyI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       DsaI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HpaII       HphI        ItaI        
MaeIII      MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NlaIII      NlaIV       PvuII       RsaI        Sau96I      
ScrFI       SfaNI       TseI        Tsp45I      TspRI       
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