Restriction map for pF1KB6570
 (Linear) (Six-base) MAPSORT of: KIBB6570.seq  Check: 2325  from: 1  to: 1200



KIBB6570.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     805    1020    1200 
   Size:        805     215     180

  Fragments arranged by size: 

                 805     215     180
 
AccI GT'mk_AC

Cuts at:      0     422    1200 
   Size:        422     778
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1039    1045    1200 
   Size:       1039       6     155

  Fragments arranged by size: 

                1039     155       6
 
AfeI AGC'GCT

Cuts at:      0     609    1200 
   Size:        609     591
 
AflIII A'CryG_T

Cuts at:      0    1010    1200 
   Size:       1010     190
 
AleI CACnn'nnGTG

Cuts at:      0     895    1120    1200 
   Size:        895     225      80

  Fragments arranged by size: 

                 895     225      80
 
AlwNI CAG_nnn'CTG

Cuts at:      0     476    1200 
   Size:        476     724
 
ApaI G_GGCC'C

Cuts at:      0     922    1200 
   Size:        922     278
 
ApaLI G'TGCA_C

Cuts at:      0     328     862    1200 
   Size:        328     534     338

  Fragments arranged by size: 

                 534     338     328
 
AvaI C'yCGr_G

Cuts at:      0     562    1200 
   Size:        562     638
 
BanI G'GyrC_C

Cuts at:      0     696    1200 
   Size:        696     504
 
BanII G_rGCy'C

Cuts at:      0     466     922    1200 
   Size:        466     456     278

  Fragments arranged by size: 

                 466     456     278
 
BbvCI CC'TCA_GC

Cuts at:      0     316     378    1200 
   Size:        316      62     822

  Fragments arranged by size: 

                 822     316      62
 
BlpI GC'TnA_GC

Cuts at:      0    1055    1200 
   Size:       1055     145
 
Bme1580I G_kGCm'C

Cuts at:      0     332     701     866     922    1200 
   Size:        332     369     165      56     278

  Fragments arranged by size: 

                 369     332     278     165      56
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      78    1200 
   Size:         78    1122
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     140    1200 
   Size:        140    1060
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294    1200 
   Size:        294     906
 
Bpu10I CC'TnA_GC

Cuts at:      0     115     316     378     465    1200 
   Size:        115     201      62      87     735

  Fragments arranged by size: 

                 735     201     115      87      62
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     652    1200 
   Size:        652     548
 
BsaAI yAC'GTr

Cuts at:      0     333    1200 
   Size:        333     867
 
BsaWI w'CCGG_w

Cuts at:      0     188    1200 
   Size:        188    1012
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      99     226     366    1200 
   Size:         99     127     140     834

  Fragments arranged by size: 

                 834     140     127      99
 
BseYI C'CCAG_C

Cuts at:      0     748     758    1200 
   Size:        748      10     442

  Fragments arranged by size: 

                 748     442      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112    1200 
   Size:        112    1088
 
BsiHKAI G_wGCw'C

Cuts at:      0     332     593     866    1200 
   Size:        332     261     273     334

  Fragments arranged by size: 

                 334     332     273     261
 
Bsp1286I G_dGCh'C

Cuts at:      0     332     466     593     701     866     922    1200 
   Size:        332     134     127     108     165      56     278

  Fragments arranged by size: 

                 332     278     165     134     127     108      56
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     805    1020    1200 
   Size:        805     215     180

  Fragments arranged by size: 

                 805     215     180
 
BsrFI r'CCGG_y

Cuts at:      0      42    1038    1200 
   Size:         42     996     162

  Fragments arranged by size: 

                 996     162      42
 
BsrGI T'GTAC_A

Cuts at:      0      34    1200 
   Size:         34    1166
 
BssHII G'CGCG_C

Cuts at:      0     703    1200 
   Size:        703     497
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     476    1200 
   Size:        476     724
 
BstYI r'GATC_y

Cuts at:      0     254    1200 
   Size:        254     946
 
BtgI C'CryG_G

Cuts at:      0     229     356     635     692     882    1076    1200 
   Size:        229     127     279      57     190     194     124

  Fragments arranged by size: 

                 279     229     194     190     127     124      57
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      36      95     528    1200 
   Size:         36      59     433     672

  Fragments arranged by size: 

                 672     433      59      36
 
DraIII CAC_nnn'GTG

Cuts at:      0     861    1200 
   Size:        861     339
 
EaeI y'GGCC_r

Cuts at:      0     204    1073    1200 
   Size:        204     869     127

  Fragments arranged by size: 

                 869     204     127
 
EarI CTCTTCn'nnn_

Cuts at:      0      80    1200 
   Size:         80    1120
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     727    1200 
   Size:        727     473
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294    1039    1045    1200 
   Size:        294     745       6     155

  Fragments arranged by size: 

                 745     294     155       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     297     919    1132    1171    1200 
   Size:        297     622     213      39      29

  Fragments arranged by size: 

                 622     297     213      39      29
 
FseI GG_CCGG'CC

Cuts at:      0    1042    1200 
   Size:       1042     158
 
HaeII r_GCGC'y

Cuts at:      0      43     476     611    1200 
   Size:         43     433     135     589

  Fragments arranged by size: 

                 589     433     135      43
 
HincII GTy'rAC

Cuts at:      0      48    1200 
   Size:         48    1152
 
MscI TGG'CCA

Cuts at:      0    1075    1200 
   Size:       1075     125
 
MslI CAynn'nnrTG

Cuts at:      0     598     895    1120    1200 
   Size:        598     297     225      80

  Fragments arranged by size: 

                 598     297     225      80
 
MspA1I CmG'CkG

Cuts at:      0      18     231     260     303     320     382     725     762 
   Size:         18     213      29      43      17      62     343      37

Cuts at:    762    1054    1195    1200 
   Size:        292     141       5

  Fragments arranged by size: 

                 343     292     213     141      62      43      37      29
                  18      17       5
 
NaeI GCC'GGC

Cuts at:      0    1040    1200 
   Size:       1040     160
 
NcoI C'CATG_G

Cuts at:      0    1076    1200 
   Size:       1076     124
 
NgoMIV G'CCGG_C

Cuts at:      0    1038    1200 
   Size:       1038     162
 
NspI r_CATG'y

Cuts at:      0     645    1200 
   Size:        645     555
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     842    1167    1200 
   Size:        842     325      33

  Fragments arranged by size: 

                 842     325      33
 
PfoI T'CCnGG_A

Cuts at:      0     731    1142    1200 
   Size:        731     411      58

  Fragments arranged by size: 

                 731     411      58
 
PmlI CAC'GTG

Cuts at:      0     333    1200 
   Size:        333     867
 
PpuMI rG'GwC_Cy

Cuts at:      0     297    1171    1200 
   Size:        297     874      29

  Fragments arranged by size: 

                 874     297      29
 
PspOMI G'GGCC_C

Cuts at:      0     918    1200 
   Size:        918     282
 
PstI C_TGCA'G

Cuts at:      0     239    1031    1200 
   Size:        239     792     169

  Fragments arranged by size: 

                 792     239     169
 
PvuII CAG'CTG

Cuts at:      0     320     762    1054    1195    1200 
   Size:        320     442     292     141       5

  Fragments arranged by size: 

                 442     320     292     141       5
 
SacII CC_GC'GG

Cuts at:      0     232    1200 
   Size:        232     968
 
SanDI GG'GwC_CC

Cuts at:      0     297    1200 
   Size:        297     903
 
SapI GCTCTTCn'nnn_

Cuts at:      0      80    1200 
   Size:         80    1120
 
ScaI AGT'ACT

Cuts at:      0      67    1200 
   Size:         67    1133
 
SexAI A'CCwGG_T

Cuts at:      0     857    1200 
   Size:        857     343
 
SfcI C'TryA_G

Cuts at:      0     235    1027    1200 
   Size:        235     792     173

  Fragments arranged by size: 

                 792     235     173
 
SphI G_CATG'C

Cuts at:      0     645    1200 
   Size:        645     555
 
StuI AGG'CCT

Cuts at:      0     241    1200 
   Size:        241     959
 
StyI C'CwwG_G

Cuts at:      0     893    1076    1200 
   Size:        893     183     124

  Fragments arranged by size: 

                 893     183     124
 
TatI w'GTAC_w

Cuts at:      0      34      65    1200 
   Size:         34      31    1135

  Fragments arranged by size: 

                1135      34      31
 
XmnI GAAnn'nnTTC

Cuts at:      0     349    1200 
   Size:        349     851
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AleI    AlwNI     ApaI
    ApaLI     AvaI     BanI    BanII    BbvCI     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaWI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspMI    BsrFI    BsrGI   BssHII   BstAPI
    BstYI     BtgI    BtgZI   DraIII     EaeI     EarI     EciI  Eco57MI
 EcoO109I     FseI    HaeII   HincII     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NspI    PflMI     PfoI     PmlI    PpuMI   PspOMI
     PstI    PvuII    SacII    SanDI     SapI     ScaI    SexAI     SfcI
     SphI     StuI     StyI     TatI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AlfI     AloI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII    BmgBI
     BmtI    BpuEI    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI     DrdI
     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FspI    FspAI
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI     SalI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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