Restriction map for pF1KB6566
 (Linear) (Six-base) MAPSORT of: KIBB6566.seq  Check: 5573  from: 1  to: 1200



KIBB6566.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

Acc65I G'GTAC_C

Cuts at:      0     344    1200 
   Size:        344     856
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640     661     821     845     892    1200 
   Size:        640      21     160      24      47     308

  Fragments arranged by size: 

                 640     308     160      47      24      21
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1107    1200 
   Size:       1107      93
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1145    1200 
   Size:       1145      55
 
AlwNI CAG_nnn'CTG

Cuts at:      0     408     522    1020    1200 
   Size:        408     114     498     180

  Fragments arranged by size: 

                 498     408     180     114
 
ApaI G_GGCC'C

Cuts at:      0     368    1200 
   Size:        368     832
 
AvaI C'yCGr_G

Cuts at:      0     171    1150    1200 
   Size:        171     979      50

  Fragments arranged by size: 

                 979     171      50
 
BanI G'GyrC_C

Cuts at:      0     344     427     724    1200 
   Size:        344      83     297     476

  Fragments arranged by size: 

                 476     344     297      83
 
BanII G_rGCy'C

Cuts at:      0     118     368     999    1200 
   Size:        118     250     631     201

  Fragments arranged by size: 

                 631     250     201     118
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     968    1200 
   Size:        968     232
 
BbvCI CC'TCA_GC

Cuts at:      0     673    1200 
   Size:        673     527
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     714     748    1131    1165    1200 
   Size:        714      34     383      34      35

  Fragments arranged by size: 

                 714     383      35      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     144    1200 
   Size:        144    1056
 
BclI T'GATC_A

Cuts at:      0    1022    1200 
   Size:       1022     178
 
BglI GCCn_nnn'nGGC

Cuts at:      0      56     304     478    1003    1200 
   Size:         56     248     174     525     197

  Fragments arranged by size: 

                 525     248     197     174      56
 
BlpI GC'TnA_GC

Cuts at:      0     940    1200 
   Size:        940     260
 
Bme1580I G_kGCm'C

Cuts at:      0     368     727    1200 
   Size:        368     359     473

  Fragments arranged by size: 

                 473     368     359
 
BmgBI CAC'GTC

Cuts at:      0      35    1200 
   Size:         35    1165
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     429     619     850    1138    1200 
   Size:        322     107     190     231     288      62

  Fragments arranged by size: 

                 322     288     231     190     107      62
 
Bpu10I CC'TnA_GC

Cuts at:      0     673    1200 
   Size:        673     527
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     937    1200 
   Size:        937     263
 
BsaBI GATnn'nnATC

Cuts at:      0     607    1200 
   Size:        607     593
 
BsaHI Gr'CG_yC

Cuts at:      0     200    1200 
   Size:        200    1000
 
BsaWI w'CCGG_w

Cuts at:      0     788    1200 
   Size:        788     412
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     100     130     680     710    1200 
   Size:        100      30     550      30     490

  Fragments arranged by size: 

                 550     490     100      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     159     670    1200 
   Size:        159     511     530

  Fragments arranged by size: 

                 530     511     159
 
BseYI C'CCAG_C

Cuts at:      0     100     998    1040    1044    1200 
   Size:        100     898      42       4     156

  Fragments arranged by size: 

                 898     156     100      42       4
 
BsiEI CG_ry'CG

Cuts at:      0     126     267    1200 
   Size:        126     141     933

  Fragments arranged by size: 

                 933     141     126
 
BsmI GAATG_Cn'

Cuts at:      0     104    1200 
   Size:        104    1096
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     502    1200 
   Size:        502     698
 
Bsp1286I G_dGCh'C

Cuts at:      0     118     368     727     999    1200 
   Size:        118     250     359     272     201

  Fragments arranged by size: 

                 359     272     250     201     118
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     416     866     902    1200 
   Size:        416     450      36     298

  Fragments arranged by size: 

                 450     416     298      36
 
BsrBI CCG'CTC

Cuts at:      0     718    1071    1200 
   Size:        718     353     129

  Fragments arranged by size: 

                 718     353     129
 
BssHII G'CGCG_C

Cuts at:      0      95     294     985    1200 
   Size:         95     199     691     215

  Fragments arranged by size: 

                 691     215     199      95
 
BstYI r'GATC_y

Cuts at:      0     332     740    1200 
   Size:        332     408     460

  Fragments arranged by size: 

                 460     408     332
 
BtgI C'CryG_G

Cuts at:      0     126    1200 
   Size:        126    1074
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1064    1200 
   Size:       1064     136
 
EaeI y'GGCC_r

Cuts at:      0      29     123     308     584     776     849    1200 
   Size:         29      94     185     276     192      73     351

  Fragments arranged by size: 

                 351     276     192     185      94      73      29
 
EagI C'GGCC_G

Cuts at:      0     123    1200 
   Size:        123    1077
 
EarI CTCTTCn'nnn_

Cuts at:      0     620    1200 
   Size:        620     580
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     135    1200 
   Size:        135    1065
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     429     619     640     661     821     845     850 
   Size:        322     107     190      21      21     160      24       5

Cuts at:    850     892    1138    1200 
   Size:         42     246      62

  Fragments arranged by size: 

                 322     246     190     160     107      62      42      24
                  21      21       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     286    1150    1200 
   Size:        286     864      50

  Fragments arranged by size: 

                 864     286      50
 
EcoO109I rG'GnC_Cy

Cuts at:      0     176     358     365     405    1194    1200 
   Size:        176     182       7      40     789       6

  Fragments arranged by size: 

                 789     182     176      40       7       6
 
FspI TGC'GCA

Cuts at:      0     526     559    1200 
   Size:        526      33     641

  Fragments arranged by size: 

                 641     526      33
 
HaeII r_GCGC'y

Cuts at:      0    1021    1200 
   Size:       1021     179
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     680     712     916     948    1093    1125    1200 
   Size:        680      32     204      32     145      32      75

  Fragments arranged by size: 

                 680     204     145      75      32      32      32
 
KpnI G_GTAC'C

Cuts at:      0     348    1200 
   Size:        348     852
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     239    1200 
   Size:        239     961
 
MscI TGG'CCA

Cuts at:      0     586     851    1200 
   Size:        586     265     349

  Fragments arranged by size: 

                 586     349     265
 
MslI CAynn'nnrTG

Cuts at:      0     532    1200 
   Size:        532     668
 
MspA1I CmG'CkG

Cuts at:      0     128     939    1002    1028    1044    1092    1200 
   Size:        128     811      63      26      16      48     108

  Fragments arranged by size: 

                 811     128     108      63      48      26      16
 
NotI GC'GGCC_GC

Cuts at:      0     123    1200 
   Size:        123    1077
 
NspI r_CATG'y

Cuts at:      0     756    1200 
   Size:        756     444
 
PfoI T'CCnGG_A

Cuts at:      0     401     445    1200 
   Size:        401      44     755

  Fragments arranged by size: 

                 755     401      44
 
PpuMI rG'GwC_Cy

Cuts at:      0     405    1194    1200 
   Size:        405     789       6

  Fragments arranged by size: 

                 789     405       6
 
PspOMI G'GGCC_C

Cuts at:      0     364    1200 
   Size:        364     836
 
PspXI vC'TCGA_Gb

Cuts at:      0    1150    1200 
   Size:       1150      50
 
PstI C_TGCA'G

Cuts at:      0     461     486     932    1200 
   Size:        461      25     446     268

  Fragments arranged by size: 

                 461     446     268      25
 
PvuII CAG'CTG

Cuts at:      0     939    1002    1044    1200 
   Size:        939      63      42     156

  Fragments arranged by size: 

                 939     156      63      42
 
SacII CC_GC'GG

Cuts at:      0     129    1200 
   Size:        129    1071
 
SapI GCTCTTCn'nnn_

Cuts at:      0     620    1200 
   Size:        620     580
 
SfcI C'TryA_G

Cuts at:      0       9     378     457     482     928    1200 
   Size:          9     369      79      25     446     272

  Fragments arranged by size: 

                 446     369     272      79      25       9
 
SmlI C'TyrA_G

Cuts at:      0     916    1150    1200 
   Size:        916     234      50

  Fragments arranged by size: 

                 916     234      50
 
StuI AGG'CCT

Cuts at:      0      50     617    1200 
   Size:         50     567     583

  Fragments arranged by size: 

                 583     567      50
 
StyI C'CwwG_G

Cuts at:      0     956    1190    1200 
   Size:        956     234      10

  Fragments arranged by size: 

                 956     234      10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     607    1200 
   Size:        607     593
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     100     132    1200 
   Size:        100      32    1068

  Fragments arranged by size: 

                1068     100      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     210    1200 
   Size:        210     990
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     594    1200 
   Size:        594     606
 
XhoI C'TCGA_G

Cuts at:      0    1150    1200 
   Size:       1150      50
 

 Enzymes that do cut:

   Acc65I     AcuI     AhdI     AlfI    AlwNI     ApaI     AvaI     BanI
    BanII     BbsI    BbvCI     BcgI    BciVI     BclI     BglI     BlpI
 Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI     BsmI    BsmBI Bsp1286I    BspMI
    BsrBI   BssHII    BstYI     BtgI    BtgZI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I     KpnI
     MmeI     MscI     MslI   MspA1I     NotI     NspI     PfoI    PpuMI
   PspOMI    PspXI     PstI    PvuII    SacII     SapI     SfcI     SmlI
     StuI     StyI    TaqII     TstI  Tth111I     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BfrBI    BglII     BmrI     BmtI     BplI
     BsaI    BsaAI     BsgI  BsiHKAI    BsiWI    BspEI    BspHI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI
    EcoRI    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI
     KasI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI     PsrI     PvuI    RsrII     SacI
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI     XbaI     XmaI     XmnI     ZraI
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