Restriction map for pF1KB6548
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BbeI        BbsI        
BcgI        BciVI       BclI        BglII       BlnI        
BpuAI       BsaWI       BseAI       BsgI        BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstUI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      DrdI        Eam1105I    Eco47III    
Eco81I      EcoNI       EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KasI        KspI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MunI        MvnI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tsp509I     Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I        406  GGTACC               G^GTACC
AcyI          898  GACGCC               G[GA]^CG[CT]C
AflII         982  CTTAAG               C^TTAAG
Asp718I       406  GGTACC               G^GTACC
AspHI          16  GTGCTC               G[AT]GC[AT]^C
BanI          406  GGTACC               G^G[CT][GA]CC
BbrPI         112  CACGTG               CAC^GTG
BfaI          394  CTAG                 C^TAG
BfrI          982  CTTAAG               C^TTAAG
BpmI           27  CTCCAG               CTGGAG(16/14)
BsaAI         112  CACGTG               [CT]AC^GT[GA]
BsaBI        1113  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         898  GACGCC               G[GA]^CG[CT]C
BsaI          198  GGTCTC               GGTCTC(1/5)
BsiEI         851  CGGCCG               CG[GA][CT]^CG
BsiHKAI        16  GTGCTC               G[AT]GC[AT]^C
BsmI           42  GAATGC               GAATGC(1/-1)
BstEII        135  GGTGACC              G^GT[ACGT]ACC
BstPI         135  GGTGACC              G^GT[ACGT]ACC
BstXI         821  CCATCCCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI          851  CGGCCG               C^GGCCG
EclXI         851  CGGCCG               C^GGCCG
Eco52I        851  CGGCCG               C^GGCCG
EcoO65I       135  GGTGACC              G^GT[ACGT]ACC
HaeII         597  GGCGCT               [GA]GCGC^[CT]
HgaI          898  GACGC                GACGC(5/10)
Hin1I         898  GACGCC               G[GA]^CG[CT]C
KpnI          406  GGTACC               GGTAC^C
MaeI          394  CTAG                 C^TAG
MamI         1113  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          983  TTAA                 T^TAA
MspA1I       1031  CAGCTG               C[AC]G^C[GT]G
NaeI          849  GCCGGC               GCC^GGC
NgoMIV        849  GCCGGC               G^CCGGC
PmaCI         112  CACGTG               CAC^GTG
PmlI          112  CACGTG               CAC^GTG
PstI          425  CTGCAG               CTGCA^G
PvuII        1031  CAGCTG               CAG^CTG
SfcI          425  CTGCAG               C^T[GA][CT]AG
SmaI          729  CCCGGG               CCC^GGG
SmlI          982  CTTAAG               C^T[CT][GA]AG
Tru9I         983  TTAA                 T^TAA
XmaCI         729  CCCGGG               C^CCGGG
XmaI          729  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflIII        111  ACACGT               A^C[GA][CT]GT
              767  ACGTGT              
ApaI          871  GGGCCC               GGGCC^C
             1050  GGGCCC              
AvaI          175  CCCGAG               C^[CT]CG[GA]G
              729  CCCGGG              
BbvCI         885  CCTCAGC              CCTCAGC(-5/-2)
              571  GCTGAGG             
BglI          367  GCCGAGTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              554  GCCTGAAGGGC         
BlpI          958  GCTGAGC              GC^T[ACGT]AGC
             1006  GCTAAGC             
Bpu1102I      958  GCTGAGC              GC^T[ACGT]AGC
             1006  GCTAAGC             
BsmFI         896  GGGAC                GGGAC(10/14)
                6  GTCCC               
BsoBI         175  CCCGAG               C^[CT]CG[GA]G
              729  CCCGGG              
BsrFI         849  GCCGGC               [GA]^CCGG[CT]
             1011  GCCGGT              
CelII         958  GCTGAGC              GC^T[ACGT]AGC
             1006  GCTAAGC             
Cfr10I        849  GCCGGC               [GA]^CCGG[CT]
             1011  GCCGGT              
DsaI          776  CCATGG               C^C[GA][CT]GG
             1103  CCATGG              
FauI          815  GCGGG                CCCGC(4/6)
             1048  GCGGG               
MaeII         113  ACGT                 A^CGT
              767  ACGT                
NcoI          776  CCATGG               C^CATGG
             1103  CCATGG              
NlaIII        777  CATG                 CATG^
             1104  CATG                
PleI          835  GACTC                GAGTC(4/5)
              304  GAGTC               
PpuMI         287  AGGTCCT              [GA]G^G[AT]CC[CT]
              752  AGGACCC             
SfaNI         364  GATGC                GCATC(5/9)
              568  GATGC               
TaqI           59  TCGA                 T^CGA
              500  TCGA                
TspRI          51  CACTG                CA[GC]TG[ACGT][ACGT]^
              998  CAGTG               
TthHB8I        59  TCGA                 T^CGA
              500  TCGA                
XcmI          989  CCAAGCCACCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1103  CCATGGACGAGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BanII       BbvI        BcnI        BmyI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MflI        MnlI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIV       PflMI       
RsaI        Sau3AI      Sau96I      ScrFI       StyI        
TfiI        TseI        Tsp45I      Van91I      XhoII       
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