Restriction map for pF1KB6548
 (Linear) (Six-base) MAPSORT of: KIBB6548.seq  Check: 3117  from: 1  to: 1176



KIBB6548.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

Acc65I G'GTAC_C

Cuts at:      0     406    1176 
   Size:        406     770
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22     577     808    1079    1176 
   Size:         22     555     231     271      97

  Fragments arranged by size: 

                 555     271     231      97      22
 
AflII C'TTAA_G

Cuts at:      0     982    1176 
   Size:        982     194
 
AflIII A'CryG_T

Cuts at:      0     111     767    1176 
   Size:        111     656     409

  Fragments arranged by size: 

                 656     409     111
 
AlwNI CAG_nnn'CTG

Cuts at:      0     129     240     424     555     945    1176 
   Size:        129     111     184     131     390     231

  Fragments arranged by size: 

                 390     231     184     131     129     111
 
ApaI G_GGCC'C

Cuts at:      0     875    1054    1176 
   Size:        875     179     122

  Fragments arranged by size: 

                 875     179     122
 
AvaI C'yCGr_G

Cuts at:      0     175     729    1176 
   Size:        175     554     447

  Fragments arranged by size: 

                 554     447     175
 
BanI G'GyrC_C

Cuts at:      0     406    1176 
   Size:        406     770
 
BanII G_rGCy'C

Cuts at:      0     434     722     875     965    1054    1176 
   Size:        434     288     153      90      89     122

  Fragments arranged by size: 

                 434     288     153     122      90      89
 
BbvCI CC'TCA_GC

Cuts at:      0     572     886    1176 
   Size:        572     314     290

  Fragments arranged by size: 

                 572     314     290
 
BglI GCCn_nnn'nGGC

Cuts at:      0     373     560    1176 
   Size:        373     187     616

  Fragments arranged by size: 

                 616     373     187
 
BlpI GC'TnA_GC

Cuts at:      0     959    1007    1176 
   Size:        959      48     169

  Fragments arranged by size: 

                 959     169      48
 
Bme1580I G_kGCm'C

Cuts at:      0      74     875    1054    1176 
   Size:         74     801     179     122

  Fragments arranged by size: 

                 801     179     122      74
 
BmgBI CAC'GTC

Cuts at:      0     768    1176 
   Size:        768     408
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      12    1176 
   Size:         12    1164
 
Bpu10I CC'TnA_GC

Cuts at:      0     572     886    1176 
   Size:        572     314     290

  Fragments arranged by size: 

                 572     314     290
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     204    1176 
   Size:        204     972
 
BsaAI yAC'GTr

Cuts at:      0     114    1176 
   Size:        114    1062
 
BsaBI GATnn'nnATC

Cuts at:      0    1117    1176 
   Size:       1117      59
 
BsaHI Gr'CG_yC

Cuts at:      0     899    1176 
   Size:        899     277
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     754     784    1176 
   Size:        754      30     392

  Fragments arranged by size: 

                 754     392      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     238     336     526     601     604     607     712    1176 
   Size:        238      98     190      75       3       3     105     464

  Fragments arranged by size: 

                 464     238     190     105      98      75       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0      74     880     977    1069    1165    1176 
   Size:         74     806      97      92      96      11

  Fragments arranged by size: 

                 806      97      96      92      74      11
 
BsiEI CG_ry'CG

Cuts at:      0     854    1176 
   Size:        854     322
 
BsiHKAI G_wGCw'C

Cuts at:      0      20    1176 
   Size:         20    1156
 
BsmI GAATG_Cn'

Cuts at:      0      48    1176 
   Size:         48    1128
 
Bsp1286I G_dGCh'C

Cuts at:      0      20      74     434     722     875     965    1054    1176 
   Size:         20      54     360     288     153      90      89     122

  Fragments arranged by size: 

                 360     288     153     122      90      89      54      20
            
 
BsrFI r'CCGG_y

Cuts at:      0     849    1011    1176 
   Size:        849     162     165

  Fragments arranged by size: 

                 849     165     162
 
BstEII G'GTnAC_C

Cuts at:      0     135    1176 
   Size:        135    1041
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     828    1176 
   Size:        828     348
 
BstYI r'GATC_y

Cuts at:      0     125     341     412    1094    1176 
   Size:        125     216      71     682      82

  Fragments arranged by size: 

                 682     216     125      82      71
 
BtgI C'CryG_G

Cuts at:      0     776    1103    1176 
   Size:        776     327      73

  Fragments arranged by size: 

                 776     327      73
 
EaeI y'GGCC_r

Cuts at:      0     161     653     851    1176 
   Size:        161     492     198     325

  Fragments arranged by size: 

                 492     325     198     161
 
EagI C'GGCC_G

Cuts at:      0     851    1176 
   Size:        851     325
 
EarI CTCTTCn'nnn_

Cuts at:      0     485     527     920    1176 
   Size:        485      42     393     256

  Fragments arranged by size: 

                 485     393     256      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     530    1176 
   Size:        530     646
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      12      22     577     808    1079    1176 
   Size:         12      10     555     231     271      97

  Fragments arranged by size: 

                 555     271     231      97      12      10
 
EcoO109I rG'GnC_Cy

Cuts at:      0     288     753     871    1176 
   Size:        288     465     118     305

  Fragments arranged by size: 

                 465     305     288     118
 
HaeII r_GCGC'y

Cuts at:      0     601    1176 
   Size:        601     575
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     754     757     786     789    1176 
   Size:        754       3      29       3     387

  Fragments arranged by size: 

                 754     387      29       3       3
 
KpnI G_GTAC'C

Cuts at:      0     410    1176 
   Size:        410     766
 
MspA1I CmG'CkG

Cuts at:      0    1033    1176 
   Size:       1033     143
 
NaeI GCC'GGC

Cuts at:      0     851    1176 
   Size:        851     325
 
NcoI C'CATG_G

Cuts at:      0     776    1103    1176 
   Size:        776     327      73

  Fragments arranged by size: 

                 776     327      73
 
NgoMIV G'CCGG_C

Cuts at:      0     849    1176 
   Size:        849     327
 
PasI CC'CwG_GG

Cuts at:      0     418     828    1176 
   Size:        418     410     348

  Fragments arranged by size: 

                 418     410     348
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     129     195    1104    1176 
   Size:        129      66     909      72

  Fragments arranged by size: 

                 909     129      72      66
 
PfoI T'CCnGG_A

Cuts at:      0     314     455    1133    1176 
   Size:        314     141     678      43

  Fragments arranged by size: 

                 678     314     141      43
 
PmlI CAC'GTG

Cuts at:      0     114    1176 
   Size:        114    1062
 
PpuMI rG'GwC_Cy

Cuts at:      0     288     753    1176 
   Size:        288     465     423

  Fragments arranged by size: 

                 465     423     288
 
PspOMI G'GGCC_C

Cuts at:      0     871    1050    1176 
   Size:        871     179     126

  Fragments arranged by size: 

                 871     179     126
 
PstI C_TGCA'G

Cuts at:      0     429    1176 
   Size:        429     747
 
PvuII CAG'CTG

Cuts at:      0    1033    1176 
   Size:       1033     143
 
SfcI C'TryA_G

Cuts at:      0     425    1176 
   Size:        425     751
 
SmaI CCC'GGG

Cuts at:      0     731    1176 
   Size:        731     445
 
SmlI C'TyrA_G

Cuts at:      0     982    1176 
   Size:        982     194
 
SrfI GCCC'GGGC

Cuts at:      0     731    1176 
   Size:        731     445
 
StyI C'CwwG_G

Cuts at:      0     359     581     776     905    1103    1176 
   Size:        359     222     195     129     198      73

  Fragments arranged by size: 

                 359     222     198     195     129      73
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     820     894    1176 
   Size:        820      74     282

  Fragments arranged by size: 

                 820     282      74
 
TatI w'GTAC_w

Cuts at:      0     209    1127    1176 
   Size:        209     918      49

  Fragments arranged by size: 

                 918     209      49
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     996    1110    1176 
   Size:        996     114      66

  Fragments arranged by size: 

                 996     114      66
 
XmaI C'CCGG_G

Cuts at:      0     729    1176 
   Size:        729     447
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII   AflIII    AlwNI     ApaI     AvaI     BanI
    BanII    BbvCI     BglI     BlpI Bme1580I    BmgBI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BsrFI   BstEII    BstXI    BstYI     BtgI
     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I    HaeII    Hin4I
     KpnI   MspA1I     NaeI     NcoI   NgoMIV     PasI    PflMI     PfoI
     PmlI    PpuMI   PspOMI     PstI    PvuII     SfcI     SmaI     SmlI
     SrfI     StyI    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI
    BglII     BmrI     BmtI     BplI    BpuEI    BsaWI     BsgI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     MfeI     MluI     MmeI     MscI     MslI     NarI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SspI     StuI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com