Restriction map for pF1KB6656
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      AlwI        Aor51HI     ApaLI       
AscI        AseI        AsnI        AspEI       AviII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BbvCI       BciVI       BclI        BfrI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BseAI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BsrFI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstYI       
BstZ17I     Cfr10I      ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HincII      HindII      HindIII     HpaI        KasI        
Ksp632I     KspI        MamI        MfeI        MflI        
MluI        MroI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII        1180  GACGTC               GACGT^C
Acc65I        220  GGTACC               G^GTACC
AccII         494  CGCG                 CG^CG
AflIII       1103  ACGTGT               A^C[GA][CT]GT
AocI         1063  CCTGAGG              CC^T[ACGT]AGG
ApaI          563  GGGCCC               GGGCC^C
Asp700I      1080  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       220  GGTACC               G^GTACC
AspI         1180  GACGTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1190  CTCGGG               C^[CT]CG[GA]G
BcnI          337  CCCGG                CC^[GC]GG
BpmI          913  CTGGAG               CTGGAG(16/14)
BsaWI          28  TCCGGT               [AT]^CCGG[AT]
BsmFI        1186  GTCCC                GGGAC(10/14)
BsoBI        1190  CTCGGG               C^[CT]CG[GA]G
BsrBI         277  GAGCGG               CCGCTC(-3/-3)
BsrDI         953  GCAATG               GCAATG(2/0)
BssSI        1336  CACGAG               CACGAG(-5/-1)
BstAPI         75  GCAGGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         494  CGCG                 CG^CG
BstXI        1045  CCAGTCAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1063  CCTGAGG              CC^T[ACGT]AGG
CfoI          495  GCGC                 GCG^C
DraIII        348  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       1063  CCTGAGG              CC^T[ACGT]AGG
EcoRI        1166  GAATTC               G^AATTC
HhaI          495  GCGC                 GCG^C
HinP1I        495  GCGC                 G^CGC
KpnI          220  GGTACC               GGTAC^C
MslI          108  CACCATTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          494  CGCG                 CG^CG
NciI          337  CCCGG                CC^[GC]GG
NcoI          902  CCATGG               C^CATGG
PflFI        1180  GACGTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          485  GACTC                GAGTC(4/5)
PshAI         994  GACTTTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          592  CTGCAG               CTGCA^G
PvuII         451  CAGCTG               CAG^CTG
SexAI         740  ACCTGGT              A^CC[AT]GGT
TfiI         1118  GAATC                G^A[AT]TC
Tth111I      1180  GACGTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         1080  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AcsI         1084  AAATTC               [GA]^AATT[CT]
             1166  GAATTC              
ApoI         1084  AAATTC               [GA]^AATT[CT]
             1166  GAATTC              
AvaII          78  GGACC                G^G[AT]CC
              129  GGACC               
BanI          220  GGTACC               G^G[CT][GA]CC
             1268  GGCACC              
BanII         563  GGGCCC               G[GA]GC[CT]^C
              781  GGGCTC              
BcgI          678  CGACGCCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              920  GCAACAATTTCG        
BfaI          839  CTAG                 C^TAG
              886  CTAG                
BlpI          274  GCTGAGC              GC^T[ACGT]AGC
              411  GCTCAGC             
Bpu1102I      274  GCTGAGC              GC^T[ACGT]AGC
              411  GCTCAGC             
BsaI          938  GAGACC               GGTCTC(1/5)
             1000  GGTCTC              
BsgI         1342  CTGCAC               GTGCAG(16/14)
              745  GTGCAG              
BsmBI          98  GAGACG               CGTCTC(1/5)
              620  GAGACG              
BspHI         716  TCATGA               T^CATGA
             1253  TCATGA              
BspMI          80  ACCTGC               ACCTGC(4/8)
              830  ACCTGC              
BsrI          137  ACTGG                ACTGG(1/-1)
             1045  CCAGT               
CelII         274  GCTGAGC              GC^T[ACGT]AGC
              411  GCTCAGC             
DpnII         258  GATC                 ^GATC
              762  GATC                
HinfI         485  GACTC                G^A[ACGT]TC
             1118  GAATC               
MaeI          839  CTAG                 C^TAG
              886  CTAG                
MaeIII        121  GTCAC                ^GT[ACGT]AC
              793  GTCAC               
MboI          258  GATC                 ^GATC
              762  GATC                
MboII        1080  GAAGA                GAAGA(8/7)
             1257  GAAGA               
MseI          503  TTAA                 T^TAA
              671  TTAA                
MspA1I        451  CAGCTG               C[AC]G^C[GT]G
              555  CAGCGG              
NdeII         258  GATC                 ^GATC
              762  GATC                
PpuMI          77  AGGACCT              [GA]G^G[AT]CC[CT]
              128  AGGACCC             
RcaI          716  TCATGA               T^CATGA
             1253  TCATGA              
Sau3AI        258  GATC                 ^GATC
              762  GATC                
SfcI          592  CTGCAG               C^T[GA][CT]AG
             1300  CTACAG              
SmlI          874  CTCAAG               C^T[CT][GA]AG
             1318  CTTGAG              
StuI          303  AGGCCT               AGG^CCT
              692  AGGCCT              
TaqI          419  TCGA                 T^CGA
              936  TCGA                
Tru9I         503  TTAA                 T^TAA
              671  TTAA                
Tsp45I        121  GTCAC                ^GT[GC]AC
              793  GTCAC               
TthHB8I       419  TCGA                 T^CGA
              936  TCGA                
XcmI          149  CCAAAAACAAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              902  CCATGGAATACCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwNI       
AspHI       BalI        BbvI        BglI        BmyI        
BsaHI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DraII       
DsaI        EaeI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        Hin1I       HpaII       HphI        ItaI        
MaeII       MluNI       MnlI        MscI        MspI        
MvaI        MwoI        NlaIII      NlaIV       RsaI        
Sau96I      ScrFI       SfaNI       StyI        TseI        
Tsp509I     TspRI       
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