Restriction map for pF1KB6620
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BspHI       BsrBI       BsrDI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KasI        KspI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MunI        
MvnI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SfiI        
SfoI        SfuI        SnaBI       SpeI        SphI        
SspI        StuI        SwaI        TfiI        Tru9I       
Tth111I     XbaI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

Acc65I       1030  GGTACC               G^GTACC
AcsI          255  GAATTC               [GA]^AATT[CT]
AcyI          791  GGCGTC               G[GA]^CG[CT]C
AflIII        244  ACATGT               A^C[GA][CT]GT
AlwI          775  GGATC                GGATC(4/5)
ApoI          255  GAATTC               [GA]^AATT[CT]
Asp718I      1030  GGTACC               G^GTACC
BcgI          330  CGAGGAGATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         910  GTATCC               GTATCC(6/5)
BfaI         1234  CTAG                 C^TAG
BsaHI         791  GGCGTC               G[GA]^CG[CT]C
BsaI         1127  GAGACC               GGTCTC(1/5)
BsgI          345  CTGCAC               GTGCAG(16/14)
BsmI         1040  GCATTC               GAATGC(1/-1)
Bsp1407I      415  TGTACA               T^GTACA
BspLU11I      244  ACATGT               A^CATGT
BsrFI        1051  ACCGGC               [GA]^CCGG[CT]
BsrGI         415  TGTACA               T^GTACA
BstAPI        167  GCATCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1209  CCAGGAAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          545  GCGC                 GCG^C
Cfr10I       1051  ACCGGC               [GA]^CCGG[CT]
DdeI          204  CTCAG                C^T[ACGT]AG
DpnII         776  GATC                 ^GATC
DraIII        564  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1112  GACCACCGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1026  CCACGG               C^C[GA][CT]GG
EaeI          683  TGGCCG               [CT]^GGCC[GA]
EcoNI        1265  CCTCTAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         255  GAATTC               G^AATTC
HaeII         544  AGCGCC               [GA]GCGC^[CT]
HhaI          545  GCGC                 GCG^C
Hin1I         791  GGCGTC               G[GA]^CG[CT]C
HinP1I        545  GCGC                 G^CGC
HinfI         966  GAGTC                G^A[ACGT]TC
KpnI         1030  GGTACC               GGTAC^C
MaeI         1234  CTAG                 C^TAG
MboI          776  GATC                 ^GATC
NdeI          104  CATATG               CA^TATG
NdeII         776  GATC                 ^GATC
NspI          244  ACATGT               [GA]CATG^[CT]
PflMI         579  CCAAGGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          966  GAGTC                GAGTC(4/5)
PvuII         295  CAGCTG               CAG^CTG
SacI          463  GAGCTC               GAGCT^C
SapI          744  GAAGAGC              GCTCTTC(1/4)
Sau3AI        776  GATC                 ^GATC
SbfI          125  CCTGCAGG             CCTGCA^GG
ScaI         1101  AGTACT               AGT^ACT
SexAI         565  ACCAGGT              A^CC[AT]GGT
SgrAI        1050  CACCGGCG             C[GA]^CCGG[CT]G
Sse8387I      125  CCTGCAGG             CCTGCA^GG
SspBI         415  TGTACA               T^GTACA
Van91I        579  CCAAGGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwNI         602  CAGGCACTG            CAG[ACGT][ACGT][ACGT]^CTG
              874  CAGCCACTG           
ApaI          404  GGGCCC               GGGCC^C
             1275  GGGCCC              
BbsI          360  GAAGAC               GAAGAC(2/6)
              727  GTCTTC              
BglI          185  GCCCGCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              443  GCCCGCCTGGC         
BpuAI         360  GAAGAC               GAAGAC(2/6)
              727  GTCTTC              
BseRI         534  CTCCTC               GAGGAG(10/8)
              331  GAGGAG              
BspMI         124  ACCTGC               ACCTGC(4/8)
              625  ACCTGC              
BstF5I        222  CATCC                GGATG(2/0)
              850  CATCC               
FokI          222  CATCC                GGATG(9/13)
              850  CATCC               
HphI         1049  TCACC                GGTGA(8/7)
             1295  TCACC               
MslI          672  CATGCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1249  CACTGTGGTG          
PaeR7I        328  CTCGAG               C^TCGAG
              409  CTCGAG              
PstI          126  CTGCAG               CTGCA^G
              762  CTGCAG              
SmaI         1003  CCCGGG               CCC^GGG
             1189  CCCGGG              
TaqI          329  TCGA                 T^CGA
              410  TCGA                
Tsp45I        311  GTGAC                ^GT[GC]AC
             1048  GTCAC               
Tsp509I       256  AATT                 ^AATT
             1289  AATT                
TthHB8I       329  TCGA                 T^CGA
              410  TCGA                
XhoI          328  CTCGAG               C^TCGAG
              409  CTCGAG              
XmaCI        1003  CCCGGG               C^CCGGG
             1189  CCCGGG              
XmaI         1003  CCCGGG               C^CCGGG
             1189  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstNI       Cac8I       Cfr13I      
DraII       EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      HaeIII      HapII       HgaI        
HpaII       ItaI        Ksp632I     MaeIII      MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NlaIII      NlaIV       PpuMI       RsaI        
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TseI        TspRI       XcmI        
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