Restriction map for pF1KB6619
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AflIII      
AhdI        Alw44I      AlwI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvrII       BamHI       BbrPI       
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BsaAI       BseAI       
BsiWI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BssHII      BstAPI      BstBI       
BstXI       BstYI       ClaI        CpoI        DraI        
DrdI        Eam1105I    Eco47III    EcoNI       EcoRV       
EcoT22I     FbaI        HgaI        HincII      HindII      
HpaI        KpnI        KspI        MaeI        MfeI        
MflI        MluI        MroI        MseI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PflFI       PflMI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tru9I       
Tth111I     Van91I      XbaI        XcmI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII          88  GACGTC               GACGT^C
AccI          963  GTATAC               GT^[AC][GT]AC
AccII         771  CGCG                 CG^CG
AlwNI         282  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1188  CCTCAGG              CC^T[ACGT]AGG
Asp700I       810  GAACCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         917  TGCGCA               TGC^GCA
BbeI          349  GGCGCC               GGCGC^C
BbvI          392  GCTGC                GCAGC(8/12)
BglI           97  GCCTATCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI         504  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        1083  CGGCCG               CG[GA][CT]^CG
BsmBI        1210  CGTCTC               CGTCTC(1/5)
Bsp1407I     1073  TGTACA               T^GTACA
BspMI        1047  GCAGGT               ACCTGC(4/8)
BsrGI        1073  TGTACA               T^GTACA
Bst1107I      963  GTATAC               GTA^TAC
BstEII        702  GGTGACC              G^GT[ACGT]ACC
BstPI         702  GGTGACC              G^GT[ACGT]ACC
BstUI         771  CGCG                 CG^CG
BstZ17I       963  GTATAC               GTA^TAC
Bsu36I       1188  CCTCAGG              CC^T[ACGT]AGG
DraIII         48  CACCTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         1083  CGGCCG               C^GGCCG
EarI          748  CTCTTC               CTCTTC(1/4)
EclXI        1083  CGGCCG               C^GGCCG
Eco52I       1083  CGGCCG               C^GGCCG
Eco81I       1188  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       702  GGTGACC              G^GT[ACGT]ACC
EcoRI         445  GAATTC               G^AATTC
FseI          221  GGCCGGCC             GGCCGG^CC
FspI          917  TGCGCA               TGC^GCA
HindIII       157  AAGCTT               A^AGCTT
KasI          349  GGCGCC               G^GCGCC
Ksp632I       748  CTCTTC               CTCTTC(1/4)
MamI          504  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI          771  CGCG                 CG^CG
NaeI          222  GCCGGC               GCC^GGC
NarI          349  GGCGCC               GG^CGCC
NcoI          902  CCATGG               C^CATGG
NgoMIV        222  GCCGGC               G^CCGGC
PaeR7I        667  CTCGAG               C^TCGAG
PleI         1090  GAGTC                GAGTC(4/5)
PshAI         705  GACCAACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         390  CAGCTG               CAG^CTG
SapI          747  GCTCTTC              GCTCTTC(1/4)
SfcI           30  CTACAG               C^T[GA][CT]AG
SfoI          349  GGCGCC               GGC^GCC
SspBI        1073  TGTACA               T^GTACA
TseI          392  GCTGC                G^C[AT]GC
XhoI          667  CTCGAG               C^TCGAG
XmnI          810  GAACCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AcyI           88  GACGTC               G[GA]^CG[CT]C
              349  GGCGCC              
AgeI          455  ACCGGT               A^CCGGT
              967  ACCGGT              
AspHI         385  GAGCTC               G[AT]GC[AT]^C
              745  GAGCTC              
AvaI          667  CTCGAG               C^[CT]CG[GA]G
              833  CCCGAG              
BalI          104  TGGCCA               TGG^CCA
              114  TGGCCA              
BanII         385  GAGCTC               G[GA]GC[CT]^C
              745  GAGCTC              
BbsI          124  GAAGAC               GAAGAC(2/6)
              624  GAAGAC              
BlpI          387  GCTCAGC              GC^T[ACGT]AGC
              952  GCTGAGC             
Bpu1102I      387  GCTCAGC              GC^T[ACGT]AGC
              952  GCTGAGC             
BpuAI         124  GAAGAC               GAAGAC(2/6)
              624  GAAGAC              
BsaHI          88  GACGTC               G[GA]^CG[CT]C
              349  GGCGCC              
BsaI          847  GAGACC               GGTCTC(1/5)
              613  GGTCTC              
BseRI         208  GAGGAG               GAGGAG(10/8)
             1114  GAGGAG              
BsgI         1045  GTGCAG               GTGCAG(16/14)
             1135  GTGCAG              
BsiHKAI       385  GAGCTC               G[AT]GC[AT]^C
              745  GAGCTC              
BsoBI         667  CTCGAG               C^[CT]CG[GA]G
              833  CCCGAG              
BsrI          402  ACTGG                ACTGG(1/-1)
              231  CCAGT               
BssSI          51  CTCGTG               CACGAG(-5/-1)
               61  CTCGTG              
CelII         387  GCTCAGC              GC^T[ACGT]AGC
              952  GCTGAGC             
DpnII         370  GATC                 ^GATC
              672  GATC                
EcoT14I       740  CCTTGG               C^C[AT][AT]GG
              902  CCATGG              
HaeII         349  GGCGCC               [GA]GCGC^[CT]
              603  AGCGCC              
Hin1I          88  GACGTC               G[GA]^CG[CT]C
              349  GGCGCC              
MboI          370  GATC                 ^GATC
              672  GATC                
MluNI         104  TGGCCA               TGG^CCA
              114  TGGCCA              
MscI          104  TGGCCA               TGG^CCA
              114  TGGCCA              
MspA1I        319  CAGCGG               C[AC]G^C[GT]G
              390  CAGCTG              
NdeII         370  GATC                 ^GATC
              672  GATC                
PinAI         455  ACCGGT               A^CCGGT
              967  ACCGGT              
PpuMI         632  AGGACCC              [GA]G^G[AT]CC[CT]
              689  GGGTCCC             
SacI          385  GAGCTC               GAGCT^C
              745  GAGCTC              
Sau3AI        370  GATC                 ^GATC
              672  GATC                
SmlI          667  CTCGAG               C^T[CT][GA]AG
             1060  CTCAAG              
StuI          893  AGGCCT               AGG^CCT
              933  AGGCCT              
StyI          740  CCTTGG               C^C[AT][AT]GG
              902  CCATGG              
TfiI          504  GATTC                G^A[AT]TC
              578  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaII       BanI        BcnI        BmyI        BpmI        
BsaJI       BsaWI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrDI       BsrFI       BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DraII       DsaI        EaeI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboII       MnlI        MslI        MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       RsaI        
Sau96I      ScrFI       SfaNI       TaqI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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