Restriction map for pF1KB6619
 (Linear) (Six-base) MAPSORT of: KIBB6619.seq  Check: 6942  from: 1  to: 1236



KIBB6619.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1038    1236 
   Size:       1038     198
 
AatII G_ACGT'C

Cuts at:      0      92    1236 
   Size:         92    1144
 
AccI GT'mk_AC

Cuts at:      0     964    1236 
   Size:        964     272
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     175    1216    1236 
   Size:         91      84    1041      20

  Fragments arranged by size: 

                1041      91      84      20
 
AgeI A'CCGG_T

Cuts at:      0     455     967    1236 
   Size:        455     512     269

  Fragments arranged by size: 

                 512     455     269
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1150    1236 
   Size:       1150      86
 
AlwNI CAG_nnn'CTG

Cuts at:      0     287    1236 
   Size:        287     949
 
ApoI r'AATT_y

Cuts at:      0     445     946     994    1236 
   Size:        445     501      48     242

  Fragments arranged by size: 

                 501     445     242      48
 
AvaI C'yCGr_G

Cuts at:      0     667     833    1236 
   Size:        667     166     403

  Fragments arranged by size: 

                 667     403     166
 
BanI G'GyrC_C

Cuts at:      0     268     349     721     859    1236 
   Size:        268      81     372     138     377

  Fragments arranged by size: 

                 377     372     268     138      81
 
BanII G_rGCy'C

Cuts at:      0     389     749    1236 
   Size:        389     360     487

  Fragments arranged by size: 

                 487     389     360
 
BbeI G_GCGC'C

Cuts at:      0     353    1236 
   Size:        353     883
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     131     631    1236 
   Size:        131     500     605

  Fragments arranged by size: 

                 605     500     131
 
BglI GCCn_nnn'nGGC

Cuts at:      0     103    1236 
   Size:        103    1133
 
BlpI GC'TnA_GC

Cuts at:      0     388     953    1236 
   Size:        388     565     283

  Fragments arranged by size: 

                 565     388     283
 
Bme1580I G_kGCm'C

Cuts at:      0     271    1236 
   Size:        271     965
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     453     593     604    1198    1236 
   Size:        453     140      11     594      38

  Fragments arranged by size: 

                 594     453     140      38      11
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1045    1236 
   Size:       1045     191
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     619     841    1236 
   Size:        619     222     395

  Fragments arranged by size: 

                 619     395     222
 
BsaBI GATnn'nnATC

Cuts at:      0     508    1236 
   Size:        508     728
 
BsaHI Gr'CG_yC

Cuts at:      0      89     350    1236 
   Size:         89     261     886

  Fragments arranged by size: 

                 886     261      89
 
BsaWI w'CCGG_w

Cuts at:      0     377     455     967    1236 
   Size:        377      78     512     269

  Fragments arranged by size: 

                 512     377     269      78
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     721     751    1236 
   Size:        721      30     485

  Fragments arranged by size: 

                 721     485      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     223    1129    1236 
   Size:        223     906     107

  Fragments arranged by size: 

                 906     223     107
 
BseYI C'CCAG_C

Cuts at:      0     317     636     852    1236 
   Size:        317     319     216     384

  Fragments arranged by size: 

                 384     319     317     216
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1066    1156    1236 
   Size:       1066      90      80

  Fragments arranged by size: 

                1066      90      80
 
BsiEI CG_ry'CG

Cuts at:      0    1086    1236 
   Size:       1086     150
 
BsiHKAI G_wGCw'C

Cuts at:      0     389     749    1236 
   Size:        389     360     487

  Fragments arranged by size: 

                 487     389     360
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1216    1236 
   Size:       1216      20
 
Bsp1286I G_dGCh'C

Cuts at:      0     271     389     749    1236 
   Size:        271     118     360     487

  Fragments arranged by size: 

                 487     360     271     118
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1038    1236 
   Size:       1038     198
 
BsrDI GCAATG_nn'

Cuts at:      0      92     422     477    1236 
   Size:         92     330      55     759

  Fragments arranged by size: 

                 759     330      92      55
 
BsrFI r'CCGG_y

Cuts at:      0     222     455     967    1236 
   Size:        222     233     512     269

  Fragments arranged by size: 

                 512     269     233     222
 
BsrGI T'GTAC_A

Cuts at:      0    1073    1236 
   Size:       1073     163
 
BssSI C'ACGA_G

Cuts at:      0      51      61    1236 
   Size:         51      10    1175

  Fragments arranged by size: 

                1175      51      10
 
BstEII G'GTnAC_C

Cuts at:      0     702    1236 
   Size:        702     534
 
BstZ17I GTA'TAC

Cuts at:      0     965    1236 
   Size:        965     271
 
Bsu36I CC'TnA_GG

Cuts at:      0    1189    1236 
   Size:       1189      47
 
BtgI C'CryG_G

Cuts at:      0      17     345     532     902    1236 
   Size:         17     328     187     370     334

  Fragments arranged by size: 

                 370     334     328     187      17
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     276     996    1236 
   Size:        276     720     240

  Fragments arranged by size: 

                 720     276     240
 
BtsI GCAGTG_nn'

Cuts at:      0    1005    1226    1236 
   Size:       1005     221      10

  Fragments arranged by size: 

                1005     221      10
 
DraIII CAC_nnn'GTG

Cuts at:      0      53    1236 
   Size:         53    1183
 
EaeI y'GGCC_r

Cuts at:      0     104     114     220     224    1083    1236 
   Size:        104      10     106       4     859     153

  Fragments arranged by size: 

                 859     153     106     104      10       4
 
EagI C'GGCC_G

Cuts at:      0    1083    1236 
   Size:       1083     153
 
EarI CTCTTCn'nnn_

Cuts at:      0     754    1236 
   Size:        754     482
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     991    1236 
   Size:        991     245
 
EcoICRI GAG'CTC

Cuts at:      0     387     747    1236 
   Size:        387     360     489

  Fragments arranged by size: 

                 489     387     360
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     175     453     593     604    1198    1216    1236 
   Size:         91      84     278     140      11     594      18      20

  Fragments arranged by size: 

                 594     278     140      91      84      20      18      11
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     284     633     690    1236 
   Size:        284     349      57     546

  Fragments arranged by size: 

                 546     349     284      57
 
EcoRI G'AATT_C

Cuts at:      0     445    1236 
   Size:        445     791
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     144    1236 
   Size:        144    1092
 
FseI GG_CCGG'CC

Cuts at:      0     226    1236 
   Size:        226    1010
 
FspI TGC'GCA

Cuts at:      0     919    1236 
   Size:        919     317
 
HaeII r_GCGC'y

Cuts at:      0     353     607    1236 
   Size:        353     254     629

  Fragments arranged by size: 

                 629     353     254
 
HindIII A'AGCT_T

Cuts at:      0     157    1236 
   Size:        157    1079
 
KasI G'GCGC_C

Cuts at:      0     349    1236 
   Size:        349     887
 
MscI TGG'CCA

Cuts at:      0     106     116    1236 
   Size:        106      10    1120

  Fragments arranged by size: 

                1120     106      10
 
MslI CAynn'nnrTG

Cuts at:      0      61     823    1132    1236 
   Size:         61     762     309     104

  Fragments arranged by size: 

                 762     309     104      61
 
MspA1I CmG'CkG

Cuts at:      0     321     392    1236 
   Size:        321      71     844

  Fragments arranged by size: 

                 844     321      71
 
NaeI GCC'GGC

Cuts at:      0     224    1236 
   Size:        224    1012
 
NarI GG'CG_CC

Cuts at:      0     350    1236 
   Size:        350     886
 
NcoI C'CATG_G

Cuts at:      0     902    1236 
   Size:        902     334
 
NgoMIV G'CCGG_C

Cuts at:      0     222    1236 
   Size:        222    1014
 
PfoI T'CCnGG_A

Cuts at:      0     581    1236 
   Size:        581     655
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     661     693    1236 
   Size:        661      32     543

  Fragments arranged by size: 

                 661     543      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     633     690    1236 
   Size:        633      57     546

  Fragments arranged by size: 

                 633     546      57
 
PshAI GACnn'nnGTC

Cuts at:      0     709    1236 
   Size:        709     527
 
PvuII CAG'CTG

Cuts at:      0     392    1236 
   Size:        392     844
 
SacI G_AGCT'C

Cuts at:      0     389     749    1236 
   Size:        389     360     487

  Fragments arranged by size: 

                 487     389     360
 
SanDI GG'GwC_CC

Cuts at:      0     690    1236 
   Size:        690     546
 
SapI GCTCTTCn'nnn_

Cuts at:      0     754    1236 
   Size:        754     482
 
SfcI C'TryA_G

Cuts at:      0      30    1236 
   Size:         30    1206
 
SfoI GGC'GCC

Cuts at:      0     351    1236 
   Size:        351     885
 
SmlI C'TyrA_G

Cuts at:      0     667    1060    1236 
   Size:        667     393     176

  Fragments arranged by size: 

                 667     393     176
 
StuI AGG'CCT

Cuts at:      0     895     935    1236 
   Size:        895      40     301

  Fragments arranged by size: 

                 895     301      40
 
StyI C'CwwG_G

Cuts at:      0     740     902    1236 
   Size:        740     162     334

  Fragments arranged by size: 

                 740     334     162
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     742    1236 
   Size:        742     494
 
TatI w'GTAC_w

Cuts at:      0    1073    1236 
   Size:       1073     163
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      37      69     721     753    1236 
   Size:         37      32     652      32     483

  Fragments arranged by size: 

                 652     483      37      32      32
 
XhoI C'TCGA_G

Cuts at:      0     667    1236 
   Size:        667     569
 
XmnI GAAnn'nnTTC

Cuts at:      0     814    1236 
   Size:        814     422
 
ZraI GAC'GTC

Cuts at:      0      90    1236 
   Size:         90    1146
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AgeI     AlfI    AlwNI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI     BglI     BlpI Bme1580I
     BpmI    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrDI
    BsrFI    BsrGI    BssSI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FseI     FspI    HaeII  HindIII     KasI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     PfoI
     PpiI    PpuMI    PshAI    PvuII     SacI    SanDI     SapI     SfcI
     SfoI     SmlI     StuI     StyI    TaqII     TatI     TstI     XhoI
     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AhdI     AleI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BsaAI    BsiWI     BsmI    BspEI    BspHI
    BsrBI   BssHII   BstAPI    BstBI    BstXI    BstYI     ClaI    CspCI
     DraI     DrdI    EcoNI    EcoRV    FspAI    Hin4I   HincII     HpaI
     KpnI     MfeI     MluI     MmeI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII
     SalI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI
     XcmI     XmaI
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