Restriction map for pF1KB6615
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiWI       BsmAI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       Bst1107I    
BstBI       BstXI       BstYI       BstZ17I     Bsu36I      
ClaI        CpoI        DpnII       DraI        DraIII      
DrdI        Eam1105I    Eco47III    Eco81I      EcoRI       
EcoRV       EcoT22I     FbaI        FspI        Hin1I       
HincII      HindII      HindIII     HinfI       HpaI        
KasI        KpnI        MamI        MboI        MfeI        
MflI        MluI        MroI        MseI        MunI        
NarI        NcoI        NdeI        NdeII       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SalI        
SapI        Sau3AI      ScaI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        TaqI        TfiI        
Tru9I       Tsp509I     Tth111I     TthHB8I     XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AspHI         664  GTGCTC               G[AT]GC[AT]^C
BbsI          901  GTCTTC               GAAGAC(2/6)
BlpI          966  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      966  GCTGAGC              GC^T[ACGT]AGC
BpuAI         901  GTCTTC               GAAGAC(2/6)
BseRI         795  CTCCTC               GAGGAG(10/8)
BsiEI         386  CGGCCG               CG[GA][CT]^CG
BsiHKAI       664  GTGCTC               G[AT]GC[AT]^C
BspMI         414  GCAGGT               ACCTGC(4/8)
BssHII        455  GCGCGC               G^CGCGC
BssSI         483  CTCGTG               CACGAG(-5/-1)
BstEII       1002  GGTCACC              G^GT[ACGT]ACC
BstPI        1002  GGTCACC              G^GT[ACGT]ACC
CelII         966  GCTGAGC              GC^T[ACGT]AGC
DdeI          967  CTGAG                C^T[ACGT]AG
EagI          386  CGGCCG               C^GGCCG
EarI          710  CTCTTC               CTCTTC(1/4)
EclXI         386  CGGCCG               C^GGCCG
Eco52I        386  CGGCCG               C^GGCCG
EcoNI          36  CCTGGTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1002  GGTCACC              G^GT[ACGT]ACC
FseI           24  GGCCGGCC             GGCCGG^CC
HaeII         465  GGCGCT               [GA]GCGC^[CT]
Ksp632I       710  CTCTTC               CTCTTC(1/4)
KspI          530  CCGCGG               CCGC^GG
MaeII         623  ACGT                 A^CGT
MslI         1020  CACTGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PpuMI         808  GGGACCT              [GA]G^G[AT]CC[CT]
SacII         530  CCGCGG               CCGC^GG
SbfI         1059  CCTGCAGG             CCTGCA^GG
SexAI          35  ACCTGGT              A^CC[AT]GGT
SfaNI        1029  GATGC                GCATC(5/9)
Sse8387I     1059  CCTGCAGG             CCTGCA^GG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AlwNI          43  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              373  CAGCTGCTG           
ApaI          167  GGGCCC               GGGCC^C
             1055  GGGCCC              
AvaI          742  CCCGAG               C^[CT]CG[GA]G
              871  CTCGGG              
BanI          331  GGTGCC               G^G[CT][GA]CC
              691  GGTGCC              
BsoBI         742  CCCGAG               C^[CT]CG[GA]G
              871  CTCGGG              
BstAPI         42  GCAGGGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              609  GCACCTTCTGC         
EcoT14I       128  CCAAGG               C^C[AT][AT]GG
              896  CCTTGG              
MboII         711  TCTTC                GAAGA(8/7)
              902  TCTTC               
NheI          444  GCTAGC               G^CTAGC
              627  GCTAGC              
NlaIII        339  CATG                 CATG^
              930  CATG                
PflMI         515  CCACCTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              821  CCAAGCACTGG         
PvuII         373  CAGCTG               CAG^CTG
             1042  CAGCTG              
SfiI         1047  GGCCCCCAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1056  GGCCCTGCAGGCC       
SmlI          154  CTCAAG               C^T[CT][GA]AG
              948  CTTGAG              
StuI          996  AGGCCT               AGG^CCT
             1134  AGGCCT              
StyI          128  CCAAGG               C^C[AT][AT]GG
              896  CCTTGG              
Van91I        515  CCACCTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              821  CCAAGCACTGG         
XcmI          338  CCATGCAGGATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              515  CCACCTACTGGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AvaII       
BalI        BanII       BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaJI       BsgI        
BsiYI       BslI        BsmFI       Bsp1286I    BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DraII       
DsaI        EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HpaII       HphI        ItaI        
MaeI        MaeIII      MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NgoMIV      NlaIV       PstI        
RsaI        Sau96I      ScrFI       SfcI        TseI        
Tsp45I      TspRI       
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