Restriction map for pF1KB6615
 (Linear) (Six-base) MAPSORT of: KIBB6615.seq  Check: 155  from: 1  to: 1236



KIBB6615.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     405    1236 
   Size:        405     831
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     427    1236 
   Size:        427     809
 
AlwNI CAG_nnn'CTG

Cuts at:      0      48     378    1236 
   Size:         48     330     858

  Fragments arranged by size: 

                 858     330      48
 
ApaI G_GGCC'C

Cuts at:      0     171    1059    1236 
   Size:        171     888     177

  Fragments arranged by size: 

                 888     177     171
 
AvaI C'yCGr_G

Cuts at:      0     742     871    1236 
   Size:        742     129     365

  Fragments arranged by size: 

                 742     365     129
 
BanI G'GyrC_C

Cuts at:      0     331     691    1236 
   Size:        331     360     545

  Fragments arranged by size: 

                 545     360     331
 
BanII G_rGCy'C

Cuts at:      0      62     171     193    1059    1236 
   Size:         62     109      22     866     177

  Fragments arranged by size: 

                 866     177     109      62      22
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     894    1236 
   Size:        894     342
 
BglI GCCn_nnn'nGGC

Cuts at:      0      57     301     875    1054    1063    1236 
   Size:         57     244     574     179       9     173

  Fragments arranged by size: 

                 574     244     179     173      57       9
 
BlpI GC'TnA_GC

Cuts at:      0     967    1236 
   Size:        967     269
 
Bme1580I G_kGCm'C

Cuts at:      0     171    1059    1123    1236 
   Size:        171     888      64     113

  Fragments arranged by size: 

                 888     171     113      64
 
BmgBI CAC'GTC

Cuts at:      0     624    1236 
   Size:        624     612
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     403    1031    1236 
   Size:        403     628     205

  Fragments arranged by size: 

                 628     403     205
 
BmtI G_CTAG'C

Cuts at:      0     448     631    1236 
   Size:        448     183     605

  Fragments arranged by size: 

                 605     448     183
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     290     400    1114    1236 
   Size:         46     244     110     714     122

  Fragments arranged by size: 

                 714     244     122     110      46
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     969    1236 
   Size:        139     830     267

  Fragments arranged by size: 

                 830     267     139
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     986    1016    1236 
   Size:        986      30     220

  Fragments arranged by size: 

                 986     220      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     786    1236 
   Size:        786     450
 
BseYI C'CCAG_C

Cuts at:      0     432     448     678    1101    1116    1222    1236 
   Size:        432      16     230     423      15     106      14

  Fragments arranged by size: 

                 432     423     230     106      16      15      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     391     433     533    1236 
   Size:         61     330      42     100     703

  Fragments arranged by size: 

                 703     330     100      61      42
 
BsiEI CG_ry'CG

Cuts at:      0     389    1236 
   Size:        389     847
 
BsiHKAI G_wGCw'C

Cuts at:      0     668    1236 
   Size:        668     568
 
Bsp1286I G_dGCh'C

Cuts at:      0      62     171     193     668    1059    1123    1236 
   Size:         62     109      22     475     391      64     113

  Fragments arranged by size: 

                 475     391     113     109      64      62      22
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     405    1236 
   Size:        405     831
 
BsrFI r'CCGG_y

Cuts at:      0      25      94     384    1236 
   Size:         25      69     290     852

  Fragments arranged by size: 

                 852     290      69      25
 
BssHII G'CGCG_C

Cuts at:      0     455    1236 
   Size:        455     781
 
BssSI C'ACGA_G

Cuts at:      0     483    1236 
   Size:        483     753
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      48     615    1236 
   Size:         48     567     621

  Fragments arranged by size: 

                 621     567      48
 
BstEII G'GTnAC_C

Cuts at:      0    1002    1236 
   Size:       1002     234
 
BtgI C'CryG_G

Cuts at:      0     145     530     726    1236 
   Size:        145     385     196     510

  Fragments arranged by size: 

                 510     385     196     145
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     771    1236 
   Size:        771     465
 
EaeI y'GGCC_r

Cuts at:      0      23     125     276     386     487     527     818    1085 
   Size:         23     102     151     110     101      40     291     267

Cuts at:   1085    1127    1236 
   Size:         42     109

  Fragments arranged by size: 

                 291     267     151     110     109     102     101      42
                  40      23
 
EagI C'GGCC_G

Cuts at:      0     386    1236 
   Size:        386     850
 
EarI CTCTTCn'nnn_

Cuts at:      0     716    1236 
   Size:        716     520
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     856    1236 
   Size:        856     380
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     290     400    1114    1236 
   Size:         46     244     110     714     122

  Fragments arranged by size: 

                 714     244     122     110      46
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      40    1236 
   Size:         40    1196
 
EcoO109I rG'GnC_Cy

Cuts at:      0      45     168     200     809     876    1055    1056    1236 
   Size:         45     123      32     609      67     179       1     180

  Fragments arranged by size: 

                 609     180     179     123      67      45      32       1
            
 
FseI GG_CCGG'CC

Cuts at:      0      29    1236 
   Size:         29    1207
 
HaeII r_GCGC'y

Cuts at:      0     469    1236 
   Size:        469     767
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     960     986     992    1018    1236 
   Size:        960      26       6      26     218

  Fragments arranged by size: 

                 960     218      26      26       6
 
MscI TGG'CCA

Cuts at:      0     127     278     820    1087    1236 
   Size:        127     151     542     267     149

  Fragments arranged by size: 

                 542     267     151     149     127
 
MslI CAynn'nnrTG

Cuts at:      0    1024    1236 
   Size:       1024     212
 
MspA1I CmG'CkG

Cuts at:      0      21     174     298     375     532    1044    1236 
   Size:         21     153     124      77     157     512     192

  Fragments arranged by size: 

                 512     192     157     153     124      77      21
 
NaeI GCC'GGC

Cuts at:      0      27      96     386    1236 
   Size:         27      69     290     850

  Fragments arranged by size: 

                 850     290      69      27
 
NgoMIV G'CCGG_C

Cuts at:      0      25      94     384    1236 
   Size:         25      69     290     852

  Fragments arranged by size: 

                 852     290      69      25
 
NheI G'CTAG_C

Cuts at:      0     444     627    1236 
   Size:        444     183     609

  Fragments arranged by size: 

                 609     444     183
 
PasI CC'CwG_GG

Cuts at:      0     801     918    1052    1236 
   Size:        801     117     134     184

  Fragments arranged by size: 

                 801     184     134     117
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     521     827    1236 
   Size:        521     306     409

  Fragments arranged by size: 

                 521     409     306
 
PfoI T'CCnGG_A

Cuts at:      0     267    1236 
   Size:        267     969
 
PpuMI rG'GwC_Cy

Cuts at:      0     809    1236 
   Size:        809     427
 
PspOMI G'GGCC_C

Cuts at:      0     167    1055    1236 
   Size:        167     888     181

  Fragments arranged by size: 

                 888     181     167
 
PstI C_TGCA'G

Cuts at:      0     983    1039    1064    1236 
   Size:        983      56      25     172

  Fragments arranged by size: 

                 983     172      56      25
 
PvuII CAG'CTG

Cuts at:      0     375    1044    1236 
   Size:        375     669     192

  Fragments arranged by size: 

                 669     375     192
 
SacII CC_GC'GG

Cuts at:      0     533    1236 
   Size:        533     703
 
SbfI CC_TGCA'GG

Cuts at:      0    1064    1236 
   Size:       1064     172
 
SexAI A'CCwGG_T

Cuts at:      0      35    1236 
   Size:         35    1201
 
SfcI C'TryA_G

Cuts at:      0     979    1035    1060    1074    1236 
   Size:        979      56      25      14     162

  Fragments arranged by size: 

                 979     162      56      25      14
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1054    1063    1236 
   Size:       1054       9     173

  Fragments arranged by size: 

                1054     173       9
 
SmlI C'TyrA_G

Cuts at:      0     154     948    1236 
   Size:        154     794     288

  Fragments arranged by size: 

                 794     288     154
 
StuI AGG'CCT

Cuts at:      0     998    1136    1236 
   Size:        998     138     100

  Fragments arranged by size: 

                 998     138     100
 
StyI C'CwwG_G

Cuts at:      0     128     896    1236 
   Size:        128     768     340

  Fragments arranged by size: 

                 768     340     128
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     195     319     670     757     793    1236 
   Size:        195     124     351      87      36     443

  Fragments arranged by size: 

                 443     351     195     124      87      36
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     345     522    1236 
   Size:        345     177     714

  Fragments arranged by size: 

                 714     345     177
 

 Enzymes that do cut:

     AarI     AlfI    AlwNI     ApaI     AvaI     BanI    BanII     BbsI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI    BpuEI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI
    BsrFI   BssHII    BssSI   BstAPI   BstEII     BtgI    BtgZI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FseI    HaeII
    Hin4I     MscI     MslI   MspA1I     NaeI   NgoMIV     NheI     PasI
    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII    SacII     SbfI
    SexAI     SfcI     SfiI     SmlI     StuI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrGI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoRI    EcoRV
     FalI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NarI     NcoI     NdeI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI
     SapI     ScaI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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