Restriction map for pF1KB6136
 (Linear) (Six-base) MAPSORT of: KIBB6136.seq  Check: 8327  from: 1  to: 1917



KIBB6136.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     666    1332    1917 
   Size:        666     666     585

  Fragments arranged by size: 

                 666     666     585
 
Acc65I G'GTAC_C

Cuts at:      0     968    1126    1917 
   Size:        968     158     791

  Fragments arranged by size: 

                 968     791     158
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     458    1021    1349    1917 
   Size:        194     264     563     328     568

  Fragments arranged by size: 

                 568     563     328     264     194
 
AhdI GACnn_n'nnGTC

Cuts at:      0     453     945    1917 
   Size:        453     492     972

  Fragments arranged by size: 

                 972     492     453
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1122    1627    1917 
   Size:       1122     505     290

  Fragments arranged by size: 

                1122     505     290
 
ApaI G_GGCC'C

Cuts at:      0     357    1360    1644    1774    1917 
   Size:        357    1003     284     130     143

  Fragments arranged by size: 

                1003     357     284     143     130
 
ApoI r'AATT_y

Cuts at:      0     193     427     649    1917 
   Size:        193     234     222    1268

  Fragments arranged by size: 

                1268     234     222     193
 
AvaI C'yCGr_G

Cuts at:      0    1645    1705    1775    1917 
   Size:       1645      60      70     142

  Fragments arranged by size: 

                1645     142      70      60
 
BamHI G'GATC_C

Cuts at:      0    1092    1917 
   Size:       1092     825
 
BanI G'GyrC_C

Cuts at:      0      37     968    1031    1126    1585    1789    1917 
   Size:         37     931      63      95     459     204     128

  Fragments arranged by size: 

                 931     459     204     128      95      63      37
 
BanII G_rGCy'C

Cuts at:      0     132     318     357     714    1360    1421    1644    1652 
   Size:        132     186      39     357     646      61     223       8

Cuts at:   1652    1713    1774    1917 
   Size:         61      61     143

  Fragments arranged by size: 

                 646     357     223     186     143     132      61      61
                  61      39       8
 
BbeI G_GCGC'C

Cuts at:      0    1035    1589    1917 
   Size:       1035     554     328

  Fragments arranged by size: 

                1035     554     328
 
BbvCI CC'TCA_GC

Cuts at:      0     479     908    1007    1543    1917 
   Size:        479     429      99     536     374

  Fragments arranged by size: 

                 536     479     429     374      99
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1686    1720    1917 
   Size:       1686      34     197

  Fragments arranged by size: 

                1686     197      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1381    1917 
   Size:       1381     536
 
BclI T'GATC_A

Cuts at:      0     999    1917 
   Size:        999     918
 
BglI GCCn_nnn'nGGC

Cuts at:      0     142     475    1594    1861    1917 
   Size:        142     333    1119     267      56

  Fragments arranged by size: 

                1119     333     267     142      56
 
Bme1580I G_kGCm'C

Cuts at:      0      68     234     357    1360    1644    1774    1917 
   Size:         68     166     123    1003     284     130     143

  Fragments arranged by size: 

                1003     284     166     143     130     123      68
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     360    1363    1917 
   Size:        360    1003     554

  Fragments arranged by size: 

                1003     554     360
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     165    1917 
   Size:        165    1752
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     706    1207    1636    1759    1830    1917 
   Size:        395     311     501     429     123      71      87

  Fragments arranged by size: 

                 501     429     395     311     123      87      71
 
Bpu10I CC'TnA_GC

Cuts at:      0     479     713     908    1007    1097    1543    1917 
   Size:        479     234     195      99      90     446     374

  Fragments arranged by size: 

                 479     446     374     234     195      99      90
 
BsaBI GATnn'nnATC

Cuts at:      0     560    1917 
   Size:        560    1357
 
BsaHI Gr'CG_yC

Cuts at:      0    1032    1586    1917 
   Size:       1032     554     331

  Fragments arranged by size: 

                1032     554     331
 
BsaWI w'CCGG_w

Cuts at:      0     691    1667    1917 
   Size:        691     976     250

  Fragments arranged by size: 

                 976     691     250
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     368     398     496     526    1435    1465    1917 
   Size:        368      30      98      30     909      30     452

  Fragments arranged by size: 

                 909     452     368      98      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     517     586     901     994     997    1917 
   Size:        517      69     315      93       3     920

  Fragments arranged by size: 

                 920     517     315      93      69       3
 
BseYI C'CCAG_C

Cuts at:      0     473     516    1360    1917 
   Size:        473      43     844     557

  Fragments arranged by size: 

                 844     557     473      43
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     716    1105    1489    1917 
   Size:        716     389     384     428

  Fragments arranged by size: 

                 716     428     389     384
 
BsiEI CG_ry'CG

Cuts at:      0     546    1917 
   Size:        546    1371
 
BsiHKAI G_wGCw'C

Cuts at:      0     714    1421    1652    1733    1816    1917 
   Size:        714     707     231      81      83     101

  Fragments arranged by size: 

                 714     707     231     101      83      81
 
BsmI GAATG_Cn'

Cuts at:      0      34     216    1917 
   Size:         34     182    1701

  Fragments arranged by size: 

                1701     182      34
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     132     234     318     357     714    1360    1421 
   Size:         68      64     102      84      39     357     646      61

Cuts at:   1421    1644    1652    1713    1733    1774    1816    1917 
   Size:        223       8      61      20      41      42     101

  Fragments arranged by size: 

                 646     357     223     102     101      84      68      64
                  61      61      42      41      39      20       8
 
BspHI T'CATG_A

Cuts at:      0      50    1917 
   Size:         50    1867
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     666    1058    1332    1917 
   Size:        666     392     274     585

  Fragments arranged by size: 

                 666     585     392     274
 
BsrBI CCG'CTC

Cuts at:      0      48    1917 
   Size:         48    1869
 
BsrDI GCAATG_nn'

Cuts at:      0      11    1171    1917 
   Size:         11    1160     746

  Fragments arranged by size: 

                1160     746      11
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1627    1694    1917 
   Size:       1627      67     223

  Fragments arranged by size: 

                1627     223      67
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     159    1917 
   Size:        159    1758
 
BstYI r'GATC_y

Cuts at:      0     576    1092    1378    1878    1917 
   Size:        576     516     286     500      39

  Fragments arranged by size: 

                 576     516     500     286      39
 
Bsu36I CC'TnA_GG

Cuts at:      0     527     569    1917 
   Size:        527      42    1348

  Fragments arranged by size: 

                1348     527      42
 
BtgI C'CryG_G

Cuts at:      0    1520    1917 
   Size:       1520     397
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1865    1917 
   Size:       1865      52
 
BtsI GCAGTG_nn'

Cuts at:      0    1477    1917 
   Size:       1477     440
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1482    1517    1917 
   Size:       1482      35     400

  Fragments arranged by size: 

                1482     400      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     417    1917 
   Size:        417    1500
 
EaeI y'GGCC_r

Cuts at:      0     590     605    1595    1917 
   Size:        590      15     990     322

  Fragments arranged by size: 

                 990     590     322      15
 
EarI CTCTTCn'nnn_

Cuts at:      0    1426    1917 
   Size:       1426     491
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     286    1917 
   Size:        286    1631
 
EcoICRI GAG'CTC

Cuts at:      0     712    1419    1650    1917 
   Size:        712     707     231     267

  Fragments arranged by size: 

                 712     707     267     231
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     395     458     706    1021    1207    1349    1636 
   Size:        194     201      63     248     315     186     142     287

Cuts at:   1636    1759    1830    1917 
   Size:        123      71      87

  Fragments arranged by size: 

                 315     287     248     201     194     186     142     123
                  87      71      63
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      91    1039    1917 
   Size:         91     948     878

  Fragments arranged by size: 

                 948     878      91
 
EcoO109I rG'GnC_Cy

Cuts at:      0      84    1047    1357    1641    1743    1770    1771    1917 
   Size:         84     963     310     284     102      27       1     146

  Fragments arranged by size: 

                 963     310     284     146     102      84      27       1
            
 
EcoRI G'AATT_C

Cuts at:      0     193     427    1917 
   Size:        193     234    1490

  Fragments arranged by size: 

                1490     234     193
 
HaeII r_GCGC'y

Cuts at:      0    1035    1225    1589    1917 
   Size:       1035     190     364     328

  Fragments arranged by size: 

                1035     364     328     190
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     439     471     931     963    1435    1467    1917 
   Size:        439      32     460      32     472      32     450

  Fragments arranged by size: 

                 472     460     450     439      32      32      32
 
HincII GTy'rAC

Cuts at:      0     774    1917 
   Size:        774    1143
 
HindIII A'AGCT_T

Cuts at:      0    1109    1917 
   Size:       1109     808
 
KasI G'GCGC_C

Cuts at:      0    1031    1585    1917 
   Size:       1031     554     332

  Fragments arranged by size: 

                1031     554     332
 
KpnI G_GTAC'C

Cuts at:      0     972    1130    1917 
   Size:        972     158     787

  Fragments arranged by size: 

                 972     787     158
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     863    1778    1917 
   Size:        863     915     139

  Fragments arranged by size: 

                 915     863     139
 
MscI TGG'CCA

Cuts at:      0     607    1597    1917 
   Size:        607     990     320

  Fragments arranged by size: 

                 990     607     320
 
MspA1I CmG'CkG

Cuts at:      0     373     473    1081    1432    1537    1694    1917 
   Size:        373     100     608     351     105     157     223

  Fragments arranged by size: 

                 608     373     351     223     157     105     100
 
NarI GG'CG_CC

Cuts at:      0    1032    1586    1917 
   Size:       1032     554     331

  Fragments arranged by size: 

                1032     554     331
 
NspI r_CATG'y

Cuts at:      0    1755    1917 
   Size:       1755     162
 
PpuMI rG'GwC_Cy

Cuts at:      0    1047    1917 
   Size:       1047     870
 
PsiI TTA'TAA

Cuts at:      0     404    1917 
   Size:        404    1513
 
PspOMI G'GGCC_C

Cuts at:      0     353    1356    1640    1770    1917 
   Size:        353    1003     284     130     147

  Fragments arranged by size: 

                1003     353     284     147     130
 
PspXI vC'TCGA_Gb

Cuts at:      0    1645    1917 
   Size:       1645     272
 
PstI C_TGCA'G

Cuts at:      0     203     584    1256    1917 
   Size:        203     381     672     661

  Fragments arranged by size: 

                 672     661     381     203
 
PvuI CG_AT'CG

Cuts at:      0     546    1917 
   Size:        546    1371
 
PvuII CAG'CTG

Cuts at:      0     373    1081    1432    1537    1694    1917 
   Size:        373     708     351     105     157     223

  Fragments arranged by size: 

                 708     373     351     223     157     105
 
SacI G_AGCT'C

Cuts at:      0     714    1421    1652    1917 
   Size:        714     707     231     265

  Fragments arranged by size: 

                 714     707     265     231
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1426    1917 
   Size:       1426     491
 
SbfI CC_TGCA'GG

Cuts at:      0     203    1917 
   Size:        203    1714
 
SfcI C'TryA_G

Cuts at:      0     199     580     839    1252    1917 
   Size:        199     381     259     413     665

  Fragments arranged by size: 

                 665     413     381     259     199
 
SfoI GGC'GCC

Cuts at:      0    1033    1587    1917 
   Size:       1033     554     330

  Fragments arranged by size: 

                1033     554     330
 
SmaI CCC'GGG

Cuts at:      0    1777    1917 
   Size:       1777     140
 
SmlI C'TyrA_G

Cuts at:      0    1645    1917 
   Size:       1645     272
 
SspI AAT'ATT

Cuts at:      0     487    1917 
   Size:        487    1430
 
StuI AGG'CCT

Cuts at:      0     108    1264    1917 
   Size:        108    1156     653

  Fragments arranged by size: 

                1156     653     108
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      88    1147    1917 
   Size:         88    1059     770

  Fragments arranged by size: 

                1059     770      88
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     368     400     496     528    1917 
   Size:        368      32      96      32    1389

  Fragments arranged by size: 

                1389     368      96      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     316     600    1917 
   Size:        316     284    1317

  Fragments arranged by size: 

                1317     316     284
 
XhoI C'TCGA_G

Cuts at:      0    1645    1917 
   Size:       1645     272
 
XmaI C'CCGG_G

Cuts at:      0    1775    1917 
   Size:       1775     142
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AhdI    AlwNI     ApaI     ApoI     AvaI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
     BglI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI   BstAPI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DrdI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MmeI     MscI   MspA1I     NarI
     NspI    PpuMI     PsiI   PspOMI    PspXI     PstI     PvuI    PvuII
     SacI     SapI     SbfI     SfcI     SfoI     SmaI     SmlI     SspI
     StuI    TaqII     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
     BbsI    BfrBI    BglII     BlpI    BmgBI     BmtI    BpuEI     BsaI
    BsaAI    BsiWI    BsmBI    BspEI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I     ClaI     DraI   DraIII     EagI    EcoRV
     FalI     FseI     FspI    FspAI     HpaI     MfeI     MluI     MslI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsrI    RsrII    SacII     SalI    SanDI     ScaI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     StyI
     SwaI     TatI  Tth111I     XbaI     XmnI     ZraI
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