Restriction map for pF1KB6107
 (Linear) (Six-base) MAPSORT of: KIBB6107.seq  Check: 6232  from: 1  to: 1740



KIBB6107.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AccI GT'mk_AC

Cuts at:      0    1337    1740 
   Size:       1337     403
 
Acc65I G'GTAC_C

Cuts at:      0     617    1740 
   Size:        617    1123
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499    1505    1740 
   Size:        499    1006     235

  Fragments arranged by size: 

                1006     499     235
 
AgeI A'CCGG_T

Cuts at:      0     641     707    1740 
   Size:        641      66    1033

  Fragments arranged by size: 

                1033     641      66
 
AhdI GACnn_n'nnGTC

Cuts at:      0     108    1740 
   Size:        108    1632
 
AleI CACnn'nnGTG

Cuts at:      0     913    1740 
   Size:        913     827
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1075    1107    1740 
   Size:       1075      32     633

  Fragments arranged by size: 

                1075     633      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     414    1710    1740 
   Size:        414    1296      30

  Fragments arranged by size: 

                1296     414      30
 
ApaI G_GGCC'C

Cuts at:      0      75     608     748    1740 
   Size:         75     533     140     992

  Fragments arranged by size: 

                 992     533     140      75
 
ApoI r'AATT_y

Cuts at:      0    1144    1286    1740 
   Size:       1144     142     454

  Fragments arranged by size: 

                1144     454     142
 
AvaI C'yCGr_G

Cuts at:      0      29     169     538     547    1528    1740 
   Size:         29     140     369       9     981     212

  Fragments arranged by size: 

                 981     369     212     140      29       9
 
AvrII C'CTAG_G

Cuts at:      0     306    1740 
   Size:        306    1434
 
BamHI G'GATC_C

Cuts at:      0      18    1740 
   Size:         18    1722
 
BanI G'GyrC_C

Cuts at:      0      43     487     617    1740 
   Size:         43     444     130    1123

  Fragments arranged by size: 

                1123     444     130      43
 
BanII G_rGCy'C

Cuts at:      0      15      75     290     548     608     692     748     884 
   Size:         15      60     215     258      60      84      56     136

Cuts at:    884    1740 
   Size:        856

  Fragments arranged by size: 

                 856     258     215     136      84      60      60      56
                  15
 
BbeI G_GCGC'C

Cuts at:      0     491    1740 
   Size:        491    1249
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     319    1740 
   Size:        319    1421
 
BbvCI CC'TCA_GC

Cuts at:      0    1230    1740 
   Size:       1230     510
 
BlpI GC'TnA_GC

Cuts at:      0    1475    1740 
   Size:       1475     265
 
Bme1580I G_kGCm'C

Cuts at:      0      75     608     748    1740 
   Size:         75     533     140     992

  Fragments arranged by size: 

                 992     533     140      75
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     249     669     885     907    1208    1740 
   Size:        249     420     216      22     301     532

  Fragments arranged by size: 

                 532     420     301     249     216      22
 
BmtI G_CTAG'C

Cuts at:      0    1262    1740 
   Size:       1262     478
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     573    1740 
   Size:        573    1167
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1257    1380    1740 
   Size:       1257     123     360

  Fragments arranged by size: 

                1257     360     123
 
Bpu10I CC'TnA_GC

Cuts at:      0    1230    1311    1740 
   Size:       1230      81     429

  Fragments arranged by size: 

                1230     429      81
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1588    1740 
   Size:       1588     152
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     698    1740 
   Size:        698    1042
 
BsaBI GATnn'nnATC

Cuts at:      0     340    1740 
   Size:        340    1400
 
BsaHI Gr'CG_yC

Cuts at:      0     488    1740 
   Size:        488    1252
 
BsaWI w'CCGG_w

Cuts at:      0     641     707    1740 
   Size:        641      66    1033

  Fragments arranged by size: 

                1033     641      66
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     115     145     277     307     535     565     920     950 
   Size:        115      30     132      30     228      30     355      30

Cuts at:    950    1075    1105    1740 
   Size:        125      30     635

  Fragments arranged by size: 

                 635     355     228     132     125     115      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     298     556     862     928    1083    1740 
   Size:        298     258     306      66     155     657

  Fragments arranged by size: 

                 657     306     298     258     155      66
 
BseYI C'CCAG_C

Cuts at:      0    1636    1740 
   Size:       1636     104
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     654     666    1529    1652    1740 
   Size:        654      12     863     123      88

  Fragments arranged by size: 

                 863     654     123      88      12
 
BsiHKAI G_wGCw'C

Cuts at:      0      15     548     884    1243    1740 
   Size:         15     533     336     359     497

  Fragments arranged by size: 

                 533     497     359     336      15
 
Bsp1286I G_dGCh'C

Cuts at:      0      15      75     290     548     608     692     748     884 
   Size:         15      60     215     258      60      84      56     136

Cuts at:    884    1243    1740 
   Size:        359     497

  Fragments arranged by size: 

                 497     359     258     215     136      84      60      60
                  56      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     629    1740 
   Size:        629    1111
 
BsrBI CCG'CTC

Cuts at:      0    1533    1740 
   Size:       1533     207
 
BsrDI GCAATG_nn'

Cuts at:      0     589     640    1740 
   Size:        589      51    1100

  Fragments arranged by size: 

                1100     589      51
 
BsrFI r'CCGG_y

Cuts at:      0     143     268     641     707     965    1740 
   Size:        143     125     373      66     258     775

  Fragments arranged by size: 

                 775     373     258     143     125      66
 
BssHII G'CGCG_C

Cuts at:      0     473    1740 
   Size:        473    1267
 
BssSI C'ACGA_G

Cuts at:      0     211     661    1740 
   Size:        211     450    1079

  Fragments arranged by size: 

                1079     450     211
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     302     677    1740 
   Size:        302     375    1063

  Fragments arranged by size: 

                1063     375     302
 
BstBI TT'CG_AA

Cuts at:      0     221    1740 
   Size:        221    1519
 
BstEII G'GTnAC_C

Cuts at:      0     364    1740 
   Size:        364    1376
 
BstYI r'GATC_y

Cuts at:      0      18     335    1740 
   Size:         18     317    1405

  Fragments arranged by size: 

                1405     317      18
 
Bsu36I CC'TnA_GG

Cuts at:      0    1691    1740 
   Size:       1691      49
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     477     550     789    1740 
   Size:        477      73     239     951

  Fragments arranged by size: 

                 951     477     239      73
 
BtsI GCAGTG_nn'

Cuts at:      0     382     405     658    1512    1740 
   Size:        382      23     253     854     228

  Fragments arranged by size: 

                 854     382     253     228      23
 
DraI TTT'AAA

Cuts at:      0    1298    1740 
   Size:       1298     442
 
DraIII CAC_nnn'GTG

Cuts at:      0     677    1740 
   Size:        677    1063
 
EaeI y'GGCC_r

Cuts at:      0    1534    1740 
   Size:       1534     206
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1000    1740 
   Size:       1000     740
 
EcoICRI GAG'CTC

Cuts at:      0      13     546     882    1740 
   Size:         13     533     336     858

  Fragments arranged by size: 

                 858     533     336      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499    1257    1380    1505    1740 
   Size:        499     758     123     125     235

  Fragments arranged by size: 

                 758     499     235     125     123
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      79     250    1061    1740 
   Size:         79     171     811     679

  Fragments arranged by size: 

                 811     679     171      79
 
EcoO109I rG'GnC_Cy

Cuts at:      0      71      72     243     744    1740 
   Size:         71       1     171     501     996

  Fragments arranged by size: 

                 996     501     171      71       1
 
EcoRI G'AATT_C

Cuts at:      0    1144    1740 
   Size:       1144     596
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     111     852    1023    1740 
   Size:        111     741     171     717

  Fragments arranged by size: 

                 741     717     171     111
 
FspI TGC'GCA

Cuts at:      0    1018    1740 
   Size:       1018     722
 
HaeII r_GCGC'y

Cuts at:      0     491    1091    1740 
   Size:        491     600     649

  Fragments arranged by size: 

                 649     600     491
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      94     126     428     460     920     952    1213    1245 
   Size:         94      32     302      32     460      32     261      32

Cuts at:   1245    1324    1356    1573    1605    1740 
   Size:         79      32     217      32     135

  Fragments arranged by size: 

                 460     302     261     217     135      94      79      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     867    1338    1740 
   Size:        867     471     402

  Fragments arranged by size: 

                 867     471     402
 
KasI G'GCGC_C

Cuts at:      0     487    1740 
   Size:        487    1253
 
KpnI G_GTAC'C

Cuts at:      0     621    1740 
   Size:        621    1119
 
MslI CAynn'nnrTG

Cuts at:      0     269     913    1740 
   Size:        269     644     827

  Fragments arranged by size: 

                 827     644     269
 
MspA1I CmG'CkG

Cuts at:      0     327    1474    1636    1740 
   Size:        327    1147     162     104

  Fragments arranged by size: 

                1147     327     162     104
 
NarI GG'CG_CC

Cuts at:      0     488    1740 
   Size:        488    1252
 
NheI G'CTAG_C

Cuts at:      0    1258    1740 
   Size:       1258     482
 
NspI r_CATG'y

Cuts at:      0     268    1449    1740 
   Size:        268    1181     291

  Fragments arranged by size: 

                1181     291     268
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     239    1254    1671    1740 
   Size:        239    1015     417      69

  Fragments arranged by size: 

                1015     417     239      69
 
PfoI T'CCnGG_A

Cuts at:      0     276    1740 
   Size:        276    1464
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1075    1107    1740 
   Size:       1075      32     633

  Fragments arranged by size: 

                1075     633      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     243    1740 
   Size:        243    1497
 
PsiI TTA'TAA

Cuts at:      0     959    1740 
   Size:        959     781
 
PspOMI G'GGCC_C

Cuts at:      0      71     604     744    1740 
   Size:         71     533     140     996

  Fragments arranged by size: 

                 996     533     140      71
 
PspXI vC'TCGA_Gb

Cuts at:      0     547    1740 
   Size:        547    1193
 
PstI C_TGCA'G

Cuts at:      0     509     890    1740 
   Size:        509     381     850

  Fragments arranged by size: 

                 850     509     381
 
PvuII CAG'CTG

Cuts at:      0    1474    1636    1740 
   Size:       1474     162     104

  Fragments arranged by size: 

                1474     162     104
 
SacI G_AGCT'C

Cuts at:      0      15     548     884    1740 
   Size:         15     533     336     856

  Fragments arranged by size: 

                 856     533     336      15
 
SalI G'TCGA_C

Cuts at:      0    1336    1740 
   Size:       1336     404
 
ScaI AGT'ACT

Cuts at:      0     835    1740 
   Size:        835     905
 
SexAI A'CCwGG_T

Cuts at:      0    1664    1709    1740 
   Size:       1664      45      31

  Fragments arranged by size: 

                1664      45      31
 
SfcI C'TryA_G

Cuts at:      0     505     886    1740 
   Size:        505     381     854

  Fragments arranged by size: 

                 854     505     381
 
SfoI GGC'GCC

Cuts at:      0     489    1740 
   Size:        489    1251
 
SmlI C'TyrA_G

Cuts at:      0     547    1603    1740 
   Size:        547    1056     137

  Fragments arranged by size: 

                1056     547     137
 
SphI G_CATG'C

Cuts at:      0     268    1740 
   Size:        268    1472
 
StuI AGG'CCT

Cuts at:      0     667    1740 
   Size:        667    1073
 
StyI C'CwwG_G

Cuts at:      0     306     424    1436    1740 
   Size:        306     118    1012     304

  Fragments arranged by size: 

                1012     306     304     118
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     688     768    1047    1740 
   Size:        688      80     279     693

  Fragments arranged by size: 

                 693     688     279      80
 
TatI w'GTAC_w

Cuts at:      0     161     752     833    1740 
   Size:        161     591      81     907

  Fragments arranged by size: 

                 907     591     161      81
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     277     309     535     567    1740 
   Size:        277      32     226      32    1173

  Fragments arranged by size: 

                1173     277     226      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1251    1404    1740 
   Size:       1251     153     336

  Fragments arranged by size: 

                1251     336     153
 
XhoI C'TCGA_G

Cuts at:      0     547    1740 
   Size:        547    1193
 
XmnI GAAnn'nnTTC

Cuts at:      0      94    1740 
   Size:         94    1646
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AgeI     AhdI     AleI     AloI    AlwNI
     ApaI     ApoI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I    BtgZI
     BtsI     DraI   DraIII     EaeI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MslI   MspA1I     NarI     NheI     NspI    PflMI     PfoI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PstI    PvuII     SacI
     SalI     ScaI    SexAI     SfcI     SfoI     SmlI     SphI     StuI
     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AlfI    ApaLI
     AscI     AseI    AsiSI     BaeI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII    BmgBI    BsaAI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrGI    BstXI  BstZ17I     BtgI     ClaI    CspCI
     DrdI     EagI     EarI    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     MluI     MmeI     MscI     NaeI     NcoI     NdeI   NgoMIV
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI
    PshAI     PsrI     PvuI    RsrII    SacII    SanDI     SapI     SbfI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     SwaI  Tth111I     XbaI     XmaI     ZraI
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