Restriction map for pF1KB5990
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

Acc65I      AccII       AccIII      AflII       AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        BanII       BbrPI       
BbvCI       BciVI       BclI        BfrI        BglI        
BglII       BmyI        BsaAI       BsaBI       BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       BstAPI      BstBI       BstEII      BstPI       
BstUI       Cfr10I      ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRV       EcoT22I     
FbaI        FseI        HgaI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SspBI       SspI        
StuI        SwaI        Tsp45I      Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII         737  GACGTC               GACGT^C
AccI          378  GTATAC               GT^[AC][GT]AC
AcsI          475  GAATTC               [GA]^AATT[CT]
AlwNI         754  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1444  CCTCAGG              CC^T[ACGT]AGG
ApoI          475  GAATTC               [GA]^AATT[CT]
Asp700I       103  GAAGGGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1322  CTCGGG               C^[CT]CG[GA]G
AviII          17  TGCGCA               TGC^GCA
AvrII        1497  CCTAGG               C^CTAGG
BamHI        1123  GGATCC               G^GATCC
BanI          223  GGCGCC               G^G[CT][GA]CC
BbeI          223  GGCGCC               GGCGC^C
BcgI          762  GCAGCATGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI         1497  CCTAGG               C^CTAGG
BlpI         1560  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1560  GCTGAGC              GC^T[ACGT]AGC
BsoBI        1322  CTCGGG               C^[CT]CG[GA]G
BsrDI         407  GCAATG               GCAATG(2/0)
Bst1107I      378  GTATAC               GTA^TAC
BstXI         791  CCAAGTTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       378  GTATAC               GTA^TAC
Bsu36I       1444  CCTCAGG              CC^T[ACGT]AGG
CelII        1560  GCTGAGC              GC^T[ACGT]AGC
DraII        1480  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII       1805  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          928  CCACGG               C^C[GA][CT]GG
Eco81I       1444  CCTCAGG              CC^T[ACGT]AGG
EcoO109I     1480  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRI         475  GAATTC               G^AATTC
FspI           17  TGCGCA               TGC^GCA
HaeII         223  GGCGCC               [GA]GCGC^[CT]
HphI          575  TCACC                GGTGA(8/7)
KasI          223  GGCGCC               G^GCGCC
MslI          536  CACATTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          223  GGCGCC               GG^CGCC
PpuMI        1480  GGGACCC              [GA]G^G[AT]CC[CT]
SbfI         1202  CCTGCAGG             CCTGCA^GG
SfoI          223  GGCGCC               GGC^GCC
SphI          125  GCATGC               GCATG^C
Sse8387I     1202  CCTGCAGG             CCTGCA^GG
TfiI         1813  GAATC                G^A[AT]TC
XmnI          103  GAAGGGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AclI          670  AACGTT               AA^CGTT
             1433  AACGTT              
AcyI          223  GGCGCC               G[GA]^CG[CT]C
              737  GACGTC              
AvaII         913  GGACC                G^G[AT]CC
             1481  GGACC               
BalI         1257  TGGCCA               TGG^CCA
             1613  TGGCCA              
BbsI         1346  GAAGAC               GAAGAC(2/6)
             1704  GAAGAC              
BcnI          192  CCGGG                CC^[GC]GG
             1484  CCCGG               
BpmI          442  CTCCAG               CTGGAG(16/14)
             1604  CTCCAG              
BpuAI        1346  GAAGAC               GAAGAC(2/6)
             1704  GAAGAC              
BsaHI         223  GGCGCC               G[GA]^CG[CT]C
              737  GACGTC              
BseRI         439  CTCCTC               GAGGAG(10/8)
             1415  CTCCTC              
BsmI          844  GAATGC               GAATGC(1/-1)
              515  GCATTC              
BstYI         495  AGATCC               [GA]^GATC[CT]
             1123  GGATCC              
CfoI           18  GCGC                 GCG^C
              224  GCGC                
EaeI         1257  TGGCCA               [CT]^GGCC[GA]
             1613  TGGCCA              
FauI          122  CCCGC                CCCGC(4/6)
             1754  GCGGG               
HapII         192  CCGG                 C^CGG
             1485  CCGG                
HhaI           18  GCGC                 GCG^C
              224  GCGC                
Hin1I         223  GGCGCC               G[GA]^CG[CT]C
              737  GACGTC              
HinP1I         18  GCGC                 G^CGC
              224  GCGC                
HpaII         192  CCGG                 C^CGG
             1485  CCGG                
MaeIII         61  GTAAC                ^GT[ACGT]AC
             1436  GTTAC               
MflI          495  AGATCC               [GA]^GATC[CT]
             1123  GGATCC              
MluNI        1257  TGGCCA               TGG^CCA
             1613  TGGCCA              
MscI         1257  TGGCCA               TGG^CCA
             1613  TGGCCA              
MspI          192  CCGG                 C^CGG
             1485  CCGG                
NciI          192  CCGGG                CC^[GC]GG
             1484  CCCGG               
NspI          125  GCATGC               [GA]CATG^[CT]
              765  GCATGT              
Psp1406I      670  AACGTT               AA^CGTT
             1433  AACGTT              
PstI          760  CTGCAG               CTGCA^G
             1203  CTGCAG              
PvuII         428  CAGCTG               CAG^CTG
              445  CAGCTG              
ScaI          954  AGTACT               AGT^ACT
             1227  AGTACT              
XhoII         495  AGATCC               [GA]^GATC[CT]
             1123  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BfaI        BsaI        BsaJI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BspMI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EarI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HinfI       ItaI        
Ksp632I     MaeI        MaeII       MboI        MboII       
MnlI        MseI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     Van91I      
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