Restriction map for pF1KB5990
 (Linear) (Six-base) MAPSORT of: KIBB5990.seq  Check: 2304  from: 1  to: 1827



KIBB5990.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      11    1210    1827 
   Size:         11    1199     617

  Fragments arranged by size: 

                1199     617      11
 
AatII G_ACGT'C

Cuts at:      0     741    1827 
   Size:        741    1086
 
AccI GT'mk_AC

Cuts at:      0     379    1827 
   Size:        379    1448
 
AclI AA'CG_TT

Cuts at:      0     671    1434    1827 
   Size:        671     763     393

  Fragments arranged by size: 

                 763     671     393
 
AlwNI CAG_nnn'CTG

Cuts at:      0     759    1827 
   Size:        759    1068
 
ApoI r'AATT_y

Cuts at:      0     475    1827 
   Size:        475    1352
 
AvaI C'yCGr_G

Cuts at:      0    1322    1827 
   Size:       1322     505
 
AvrII C'CTAG_G

Cuts at:      0    1497    1827 
   Size:       1497     330
 
BamHI G'GATC_C

Cuts at:      0    1123    1827 
   Size:       1123     704
 
BanI G'GyrC_C

Cuts at:      0     223    1827 
   Size:        223    1604
 
BbeI G_GCGC'C

Cuts at:      0     227    1827 
   Size:        227    1600
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1353    1711    1827 
   Size:       1353     358     116

  Fragments arranged by size: 

                1353     358     116
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     751     785    1827 
   Size:        751      34    1042

  Fragments arranged by size: 

                1042     751      34
 
BlpI GC'TnA_GC

Cuts at:      0    1561    1827 
   Size:       1561     266
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427    1589    1827 
   Size:        427    1162     238

  Fragments arranged by size: 

                1162     427     238
 
Bpu10I CC'TnA_GC

Cuts at:      0     556    1827 
   Size:        556    1271
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      77     918    1607    1827 
   Size:         77     841     689     220

  Fragments arranged by size: 

                 841     689     220      77
 
BsaHI Gr'CG_yC

Cuts at:      0     224     738    1827 
   Size:        224     514    1089

  Fragments arranged by size: 

                1089     514     224
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1061    1091    1300    1330    1827 
   Size:       1061      30     209      30     497

  Fragments arranged by size: 

                1061     497     209      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     430    1406    1827 
   Size:        430     976     421

  Fragments arranged by size: 

                 976     430     421
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     999    1526    1827 
   Size:         57     942     527     301

  Fragments arranged by size: 

                 942     527     301      57
 
BsmI GAATG_Cn'

Cuts at:      0     515     850    1827 
   Size:        515     335     977

  Fragments arranged by size: 

                 977     515     335
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      11     126    1210    1827 
   Size:         11     115    1084     617

  Fragments arranged by size: 

                1084     617     115      11
 
BsrDI GCAATG_nn'

Cuts at:      0     414    1827 
   Size:        414    1413
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     798    1827 
   Size:        798    1029
 
BstYI r'GATC_y

Cuts at:      0     495    1123    1827 
   Size:        495     628     704

  Fragments arranged by size: 

                 704     628     495
 
BstZ17I GTA'TAC

Cuts at:      0     380    1827 
   Size:        380    1447
 
Bsu36I CC'TnA_GG

Cuts at:      0    1445    1827 
   Size:       1445     382
 
BtgI C'CryG_G

Cuts at:      0     928    1827 
   Size:        928     899
 
BtsI GCAGTG_nn'

Cuts at:      0     684     707     987    1185    1572    1655    1827 
   Size:        684      23     280     198     387      83     172

  Fragments arranged by size: 

                 684     387     280     198     172      83      23
 
DraIII CAC_nnn'GTG

Cuts at:      0    1810    1827 
   Size:       1810      17
 
EaeI y'GGCC_r

Cuts at:      0    1257    1613    1827 
   Size:       1257     356     214

  Fragments arranged by size: 

                1257     356     214
 
EarI CTCTTCn'nnn_

Cuts at:      0     395     425     605    1827 
   Size:        395      30     180    1222

  Fragments arranged by size: 

                1222     395     180      30
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427    1589    1827 
   Size:        427    1162     238

  Fragments arranged by size: 

                1162     427     238
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1481    1827 
   Size:       1481     346
 
EcoRI G'AATT_C

Cuts at:      0     475    1827 
   Size:        475    1352
 
FspI TGC'GCA

Cuts at:      0      19    1827 
   Size:         19    1808
 
HaeII r_GCGC'y

Cuts at:      0     227    1827 
   Size:        227    1600
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     637     669    1061    1093    1111    1143    1255    1284 
   Size:        637      32     392      32      18      32     112      29

Cuts at:   1284    1287    1316    1396    1401    1428    1433    1827 
   Size:          3      29      80       5      27       5     394

  Fragments arranged by size: 

                 637     394     392     112      80      32      32      32
                  29      29      27      18       5       5       3
 
KasI G'GCGC_C

Cuts at:      0     223    1827 
   Size:        223    1604
 
MscI TGG'CCA

Cuts at:      0    1259    1615    1827 
   Size:       1259     356     212

  Fragments arranged by size: 

                1259     356     212
 
MslI CAynn'nnrTG

Cuts at:      0     540    1827 
   Size:        540    1287
 
MspA1I CmG'CkG

Cuts at:      0      71     430     447    1827 
   Size:         71     359      17    1380

  Fragments arranged by size: 

                1380     359      71      17
 
NarI GG'CG_CC

Cuts at:      0     224    1827 
   Size:        224    1603
 
NspI r_CATG'y

Cuts at:      0     129     769    1827 
   Size:        129     640    1058

  Fragments arranged by size: 

                1058     640     129
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1031    1598    1739    1827 
   Size:       1031     567     141      88

  Fragments arranged by size: 

                1031     567     141      88
 
PpuMI rG'GwC_Cy

Cuts at:      0    1481    1827 
   Size:       1481     346
 
PstI C_TGCA'G

Cuts at:      0     764    1207    1827 
   Size:        764     443     620

  Fragments arranged by size: 

                 764     620     443
 
PvuII CAG'CTG

Cuts at:      0     430     447    1827 
   Size:        430      17    1380

  Fragments arranged by size: 

                1380     430      17
 
SanDI GG'GwC_CC

Cuts at:      0    1481    1827 
   Size:       1481     346
 
SbfI CC_TGCA'GG

Cuts at:      0    1207    1827 
   Size:       1207     620
 
ScaI AGT'ACT

Cuts at:      0     956    1229    1827 
   Size:        956     273     598

  Fragments arranged by size: 

                 956     598     273
 
SfcI C'TryA_G

Cuts at:      0     264     760    1203    1391    1466    1532    1553    1827 
   Size:        264     496     443     188      75      66      21     274

  Fragments arranged by size: 

                 496     443     274     264     188      75      66      21
            
 
SfoI GGC'GCC

Cuts at:      0     225    1827 
   Size:        225    1602
 
SphI G_CATG'C

Cuts at:      0     129    1827 
   Size:        129    1698
 
StyI C'CwwG_G

Cuts at:      0      78     161     916    1497    1827 
   Size:         78      83     755     581     330

  Fragments arranged by size: 

                 755     581     330      83      78
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     592    1243    1827 
   Size:        592     651     584

  Fragments arranged by size: 

                 651     592     584
 
TatI w'GTAC_w

Cuts at:      0     954    1227    1827 
   Size:        954     273     600

  Fragments arranged by size: 

                 954     600     273
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1300    1332    1827 
   Size:       1300      32     495

  Fragments arranged by size: 

                1300     495      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     107    1827 
   Size:        107    1720
 
ZraI GAC'GTC

Cuts at:      0     739    1827 
   Size:        739    1088
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI    AlwNI     ApoI     AvaI    AvrII
    BamHI     BanI     BbeI     BbsI     BcgI     BlpI     BpmI   Bpu10I
     BsaI    BsaHI    BsaXI    BseRI     BsgI     BsmI    BspMI    BsrDI
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI   DraIII     EaeI
     EarI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I     KasI
     MscI     MslI   MspA1I     NarI     NspI    PflMI    PpuMI     PstI
    PvuII    SanDI     SbfI     ScaI     SfcI     SfoI     SphI     StyI
    TaqII     TatI     TstI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AcuI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     BaeI
    BanII    BbvCI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaWI
    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI     SapI    SexAI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI
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