Restriction map for
pF1KB5974
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
BstBI BstEII BstNI BstPI CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRII EcoRV EcoT14I
FseI FspI HaeII HgaI Hin1I
HindIII KasI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvaI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1179 GTATAC GT^[AC][GT]AC
AfaI 146 GTAC GT^AC
Alw44I 1628 GTGCAC G^TGCAC
AlwNI 1592 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 463 CCTGAGG CC^T[ACGT]AGG
ApaLI 1628 GTGCAC G^TGCAC
AspHI 1628 GTGCAC G[AT]GC[AT]^C
BamHI 324 GGATCC G^GATCC
BbsI 1442 GTCTTC GAAGAC(2/6)
BclI 1029 TGATCA T^GATCA
BcnI 1016 CCGGG CC^[GC]GG
BglII 298 AGATCT A^GATCT
BpuAI 1442 GTCTTC GAAGAC(2/6)
BsaJI 1412 CCACGG C^C[ACGT][ACGT]GG
BsiEI 128 CGGTCG CG[GA][CT]^CG
BsiHKAI 1628 GTGCAC G[AT]GC[AT]^C
BsiYI 544 CCAGTTGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 544 CCAGTTGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrDI 1205 GCAATG GCAATG(2/0)
BsrFI 61 ACCGGC [GA]^CCGG[CT]
BssKI 1016 CCGGG ^CC[ACGT]GG
Bst1107I 1179 GTATAC GTA^TAC
BstF5I 1397 CATCC GGATG(2/0)
BstXI 398 CCAAAGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1179 GTATAC GTA^TAC
Bsu36I 463 CCTGAGG CC^T[ACGT]AGG
Cfr10I 61 ACCGGC [GA]^CCGG[CT]
DraII 433 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 187 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1412 CCACGG C^C[GA][CT]GG
EaeI 5 CGGCCA [CT]^GGCC[GA]
Eco81I 463 CCTGAGG CC^T[ACGT]AGG
EcoO109I 433 GGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 1029 TGATCA T^GATCA
FokI 1397 CATCC GGATG(9/13)
HpaI 118 GTTAAC GTT^AAC
MaeII 116 ACGT A^CGT
NciI 1016 CCGGG CC^[GC]GG
NheI 1198 GCTAGC G^CTAGC
NspI 1406 GCATGC [GA]CATG^[CT]
PleI 909 GAGTC GAGTC(4/5)
PpuMI 433 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 146 GTAC GT^AC
ScrFI 1016 CCGGG CC^[ACGT]GG
SmlI 560 CTCAAG C^T[CT][GA]AG
SphI 1406 GCATGC GCATG^C
Tsp45I 785 GTCAC ^GT[GC]AC
XcmI 804 CCAAACTACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 12 CGCG CG^CG
103 CGCG
AcsI 235 GAATTC [GA]^AATT[CT]
699 GAATTC
AlwI 325 GATCC GGATC(4/5)
324 GGATC
ApoI 235 GAATTC [GA]^AATT[CT]
699 GAATTC
BmyI 525 GGGCAC G[AGT]GC[ACT]^C
1628 GTGCAC
Bsp1286I 525 GGGCAC G[AGT]GC[ACT]^C
1628 GTGCAC
BstAPI 712 GCAGATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
982 GCAGCACTTGC
BstUI 12 CGCG CG^CG
103 CGCG
BstYI 298 AGATCT [GA]^GATC[CT]
324 GGATCC
CfoI 11 GCGC GCG^C
102 GCGC
EcoRI 235 GAATTC G^AATTC
699 GAATTC
EcoT22I 419 ATGCAT ATGCA^T
731 ATGCAT
HhaI 11 GCGC GCG^C
102 GCGC
HinP1I 11 GCGC G^CGC
102 GCGC
HincII 118 GTTAAC GT[CT]^[GA]AC
642 GTCAAC
HindII 118 GTTAAC GT[CT]^[GA]AC
642 GTCAAC
HphI 192 GGTGA GGTGA(8/7)
1027 GGTGA
MaeIII 785 GTCAC ^GT[ACGT]AC
1570 GTAAC
MflI 298 AGATCT [GA]^GATC[CT]
324 GGATCC
MslI 250 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1401 CATGAGCATG
MvnI 12 CGCG CG^CG
103 CGCG
NsiI 419 ATGCAT ATGCA^T
731 ATGCAT
PstI 968 CTGCAG CTGCA^G
1157 CTGCAG
PvuII 926 CAGCTG CAG^CTG
1592 CAGCTG
TaqI 89 TCGA T^CGA
754 TCGA
TfiI 496 GATTC G^A[AT]TC
1418 GATTC
TthHB8I 89 TCGA T^CGA
754 TCGA
XhoII 298 AGATCT [GA]^GATC[CT]
324 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AvaII BbvI BfaI
BpmI BseRI BsgI BsmFI BsrI
Cac8I Cfr13I DdeI DpnII FauI
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MaeI MboI MboII MnlI
MseI MspA1I MspI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I SfaNI
SfcI Tru9I TseI Tsp509I TspRI