Restriction map for pF1KB5972
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspDI       BspEI       BspLU11I    BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       FauI        FseI        FspI        HaeII       
HaeIII      HgaI        HhaI        Hin1I       HinP1I      
HpaI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NgoMIV      NheI        NotI        NruI        NspV        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PstI        
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AlwI           16  GGATC                GGATC(4/5)
BbvI           66  GCAGC                GCAGC(8/12)
BclI            2  TGATCA               T^GATCA
BpmI          112  CTGGAG               CTGGAG(16/14)
BsaI          972  GAGACC               GGTCTC(1/5)
BsaWI        1354  TCCGGT               [AT]^CCGG[AT]
BspMI         517  ACCTGC               ACCTGC(4/8)
BsrDI        1530  CATTGC               GCAATG(2/0)
BsrI          251  CCAGT                ACTGG(1/-1)
BssKI          12  CCAGG                ^CC[ACGT]GG
BstNI          12  CCAGG                CC^[AT]GG
DraII          88  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI          196  CCATGG               C^C[GA][CT]GG
EcoO109I       88  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRII         12  CCAGG                ^CC[AT]GG
EcoT14I       196  CCATGG               C^C[AT][AT]GG
EcoT22I      1157  ATGCAT               ATGCA^T
FbaI            2  TGATCA               T^GATCA
Fnu4HI         66  GCAGC                GC^[ACGT]GC
HapII        1355  CCGG                 C^CGG
HindIII       458  AAGCTT               A^AGCTT
HpaII        1355  CCGG                 C^CGG
ItaI           66  GCAGC                GC^[ACGT]GC
MspI         1355  CCGG                 C^CGG
MvaI           12  CCAGG                CC^[AT]GG
NcoI          196  CCATGG               C^CATGG
NdeI         1061  CATATG               CA^TATG
NsiI         1157  ATGCAT               ATGCA^T
NspI         1159  GCATGT               [GA]CATG^[CT]
PacI          241  TTAATTAA             TTAAT^TAA
PleI          228  GAGTC                GAGTC(4/5)
PpuMI          88  AGGACCC              [GA]G^G[AT]CC[CT]
ScrFI          12  CCAGG                CC^[ACGT]GG
SfcI          120  CTACAG               C^T[GA][CT]AG
SspI          721  AATATT               AAT^ATT
StyI          196  CCATGG               C^C[AT][AT]GG
TseI           66  GCAGC                G^C[AT]GC
Tsp45I         37  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AluI          376  AGCT                 AG^CT
              459  AGCT                
AlwNI          67  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
              375  CAGCTTCTG           
AseI          240  ATTAAT               AT^TAAT
              244  ATTAAT              
AsnI          240  ATTAAT               AT^TAAT
              244  ATTAAT              
AvaII          75  GGACC                G^G[AT]CC
               89  GGACC               
BseRI          58  GAGGAG               GAGGAG(10/8)
              190  GAGGAG              
BsiYI          92  CCCTGTACAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               93  CCTGTACAGGG         
BslI           92  CCCTGTACAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               93  CCTGTACAGGG         
BsmAI         972  GAGAC                GTCTC(1/5)
              133  GTCTC               
BsmI         1150  GAATGC               GAATGC(1/-1)
              149  GCATTC              
Cfr13I         75  GGACC                G^G[ACGT]CC
               89  GGACC               
DpnII           3  GATC                 ^GATC
               17  GATC                
EarI          478  CTCTTC               CTCTTC(1/4)
              340  GAAGAG              
HincII       1017  GTCAAC               GT[CT]^[GA]AC
             1269  GTCAAC              
HindII       1017  GTCAAC               GT[CT]^[GA]AC
             1269  GTCAAC              
Ksp632I       478  CTCTTC               CTCTTC(1/4)
              340  GAAGAG              
MaeIII         37  GTGAC                ^GT[ACGT]AC
              500  GTTAC               
MboI            3  GATC                 ^GATC
               17  GATC                
NdeII           3  GATC                 ^GATC
               17  GATC                
PshBI         240  ATTAAT               AT^TAAT
              244  ATTAAT              
Sau3AI          3  GATC                 ^GATC
               17  GATC                
Sau96I         75  GGACC                G^G[ACGT]CC
               89  GGACC               
SmlI           53  CTCAAG               C^T[CT][GA]AG
              172  CTCAAG              
TfiI          168  GATTC                G^A[AT]TC
              634  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AcsI        AfaI        ApoI        Asp700I     BbsI        
BpuAI       BsaJI       Bsp1407I    BspHI       BsrGI       
BstF5I      DdeI        EcoRI       FokI        HinfI       
HphI        MboII       MnlI        MseI        MslI        
NlaIII      NlaIV       RcaI        RsaI        SfaNI       
SspBI       Tru9I       Tsp509I     TspRI       XmnI        
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