Restriction map for pF1KB5972
 (Linear) (Six-base) MAPSORT of: KIBB5972.seq  Check: 2213  from: 1  to: 1683



KIBB5972.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     359     990    1683 
   Size:        359     631     693

  Fragments arranged by size: 

                 693     631     359
 
AleI CACnn'nnGTG

Cuts at:      0    1217    1301    1683 
   Size:       1217      84     382

  Fragments arranged by size: 

                1217     382      84
 
AlwNI CAG_nnn'CTG

Cuts at:      0      72     380    1683 
   Size:         72     308    1303

  Fragments arranged by size: 

                1303     308      72
 
ApoI r'AATT_y

Cuts at:      0     214     471     930     956    1336    1683 
   Size:        214     257     459      26     380     347

  Fragments arranged by size: 

                 459     380     347     257     214      26
 
AseI AT'TA_AT

Cuts at:      0     241     245    1683 
   Size:        241       4    1438

  Fragments arranged by size: 

                1438     241       4
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     398     922    1383    1683 
   Size:        398     524     461     300

  Fragments arranged by size: 

                 524     461     398     300
 
BclI T'GATC_A

Cuts at:      0       2    1683 
   Size:          2    1681
 
BfrBI ATG'CAT

Cuts at:      0    1159    1683 
   Size:       1159     524
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133    1683 
   Size:        133    1550
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     157    1683 
   Size:         38     119    1526

  Fragments arranged by size: 

                1526     119      38
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     966    1683 
   Size:        966     717
 
BsaWI w'CCGG_w

Cuts at:      0    1354    1683 
   Size:       1354     329
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73     205    1683 
   Size:         73     132    1478

  Fragments arranged by size: 

                1478     132      73
 
BsmI GAATG_Cn'

Cuts at:      0     149    1156    1683 
   Size:        149    1007     527

  Fragments arranged by size: 

                1007     527     149
 
BspHI T'CATG_A

Cuts at:      0     819     903     987    1071    1683 
   Size:        819      84      84      84     612

  Fragments arranged by size: 

                 819     612      84      84      84
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     526    1683 
   Size:        526    1157
 
BsrDI GCAATG_nn'

Cuts at:      0    1529    1683 
   Size:       1529     154
 
BsrGI T'GTAC_A

Cuts at:      0      95     486    1617    1683 
   Size:         95     391    1131      66

  Fragments arranged by size: 

                1131     391      95      66
 
BtgI C'CryG_G

Cuts at:      0     196    1683 
   Size:        196    1487
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      43      78     432     467    1683 
   Size:         43      35     354      35    1216

  Fragments arranged by size: 

                1216     354      43      35      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     335     484    1683 
   Size:        335     149    1199

  Fragments arranged by size: 

                1199     335     149
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     359     990    1683 
   Size:        133     226     631     693

  Fragments arranged by size: 

                 693     631     226     133
 
EcoO109I rG'GnC_Cy

Cuts at:      0      89    1683 
   Size:         89    1594
 
EcoRI G'AATT_C

Cuts at:      0     471     930     956    1683 
   Size:        471     459      26     727

  Fragments arranged by size: 

                 727     471     459      26
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     608     640    1683 
   Size:        608      32    1043

  Fragments arranged by size: 

                1043     608      32
 
HincII GTy'rAC

Cuts at:      0    1019    1271    1683 
   Size:       1019     252     412

  Fragments arranged by size: 

                1019     412     252
 
HindIII A'AGCT_T

Cuts at:      0     458    1683 
   Size:        458    1225
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     158    1683 
   Size:        158    1525
 
MslI CAynn'nnrTG

Cuts at:      0     656     824     908     992    1065    1076    1217    1301 
   Size:        656     168      84      84      73      11     141      84

Cuts at:   1301    1683 
   Size:        382

  Fragments arranged by size: 

                 656     382     168     141      84      84      84      73
                  11
 
NcoI C'CATG_G

Cuts at:      0     196    1683 
   Size:        196    1487
 
NdeI CA'TA_TG

Cuts at:      0    1062    1683 
   Size:       1062     621
 
NsiI A_TGCA'T

Cuts at:      0    1161    1683 
   Size:       1161     522
 
NspI r_CATG'y

Cuts at:      0    1163    1683 
   Size:       1163     520
 
PacI TTA_AT'TAA

Cuts at:      0     245    1683 
   Size:        245    1438
 
PasI CC'CwG_GG

Cuts at:      0      12    1683 
   Size:         12    1671
 
PpuMI rG'GwC_Cy

Cuts at:      0      89    1683 
   Size:         89    1594
 
SfcI C'TryA_G

Cuts at:      0     120    1683 
   Size:        120    1563
 
SmlI C'TyrA_G

Cuts at:      0      53     172    1683 
   Size:         53     119    1511

  Fragments arranged by size: 

                1511     119      53
 
SspI AAT'ATT

Cuts at:      0     723    1683 
   Size:        723     960
 
StyI C'CwwG_G

Cuts at:      0     196    1683 
   Size:        196    1487
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     856    1683 
   Size:        856     827
 
TatI w'GTAC_w

Cuts at:      0      95     486    1617    1683 
   Size:         95     391    1131      66

  Fragments arranged by size: 

                1131     391      95      66
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     531     563    1683 
   Size:        531      32    1120

  Fragments arranged by size: 

                1120     531      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     167     215     261     329     376     460     638     919 
   Size:        167      48      46      68      47      84     178     281

Cuts at:    919    1003    1087    1255    1322    1423    1683 
   Size:         84      84     168      67     101     260

  Fragments arranged by size: 

                 281     260     178     168     167     101      84      84
                  84      68      67      48      47      46
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     ApoI     AseI     BbsI     BclI    BfrBI
     BpmI    BpuEI     BsaI    BsaWI    BseRI     BsmI    BspHI    BspMI
    BsrDI    BsrGI     BtgI    CspCI     EarI  Eco57MI EcoO109I    EcoRI
    Hin4I   HincII  HindIII     MmeI     MslI     NcoI     NdeI     NsiI
     NspI     PacI     PasI    PpuMI     SfcI     SmlI     SspI     StyI
    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI
     MscI   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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