Restriction map for pF1KB5967
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        AlwI        
AocI        Aor51HI     AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BamHI       BbeI        BbsI        BbvCI       BcgI        
BciVI       BfrI        BglII       BlnI        BpuAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiWI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssSI       Bst1107I    BstAPI      BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HaeII       Hin1I       
HincII      HindII      HpaI        KasI        KpnI        
Ksp632I     KspI        MamI        MfeI        MflI        
MluI        MroI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        XbaI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AccII          90  CGCG                 CG^CG
AflIII       1196  ACACGT               A^C[GA][CT]GT
AspI         1107  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        1197  CACGTG               CAC^GTG
BclI          221  TGATCA               T^GATCA
BcnI          431  CCGGG                CC^[GC]GG
BglI          427  GCCACCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          379  GCTGAGC              GC^T[ACGT]AGC
BpmI           43  CTGGAG               CTGGAG(16/14)
Bpu1102I      379  GCTGAGC              GC^T[ACGT]AGC
BsmBI         456  CGTCTC               CGTCTC(1/5)
BsmI          746  GAATGC               GAATGC(1/-1)
BssHII         89  GCGCGC               G^CGCGC
BstBI          75  TTCGAA               TT^CGAA
BstUI          90  CGCG                 CG^CG
CelII         379  GCTGAGC              GC^T[ACGT]AGC
EcoNI        1097  CCTCCTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          221  TGATCA               T^GATCA
HindIII      1127  AAGCTT               A^AGCTT
MvnI           90  CGCG                 CG^CG
NciI          431  CCGGG                CC^[GC]GG
NspV           75  TTCGAA               TT^CGAA
PaeR7I        833  CTCGAG               C^TCGAG
PflFI        1107  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1197  CACGTG               CAC^GTG
PmlI         1197  CACGTG               CAC^GTG
PstI         1426  CTGCAG               CTGCA^G
SacI          836  GAGCTC               GAGCT^C
SfuI           75  TTCGAA               TT^CGAA
SphI         1208  GCATGC               GCATG^C
Tth111I      1107  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          833  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Alw44I        409  GTGCAC               G^TGCAC
             1513  GTGCAC              
AlwNI        1084  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1221  CAGCAGCTG           
ApaI           98  GGGCCC               GGGCC^C
             1297  GGGCCC              
ApaLI         409  GTGCAC               G^TGCAC
             1513  GTGCAC              
BalI         1201  TGGCCA               TGG^CCA
             1325  TGGCCA              
BsaAI         406  TACGTG               [CT]AC^GT[GA]
             1197  CACGTG              
BspMI          62  ACCTGC               ACCTGC(4/8)
              321  ACCTGC              
BsrBI        1062  CCGCTC               CCGCTC(-3/-3)
             1280  CCGCTC              
HapII         431  CCGG                 C^CGG
              893  CCGG                
HgaI            7  GACGC                GACGC(5/10)
               52  GACGC               
HpaII         431  CCGG                 C^CGG
              893  CCGG                
MluNI        1201  TGGCCA               TGG^CCA
             1325  TGGCCA              
MscI         1201  TGGCCA               TGG^CCA
             1325  TGGCCA              
MseI          281  TTAA                 T^TAA
              673  TTAA                
MslI          213  CACGGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1236  CACCACCGTG          
MspI          431  CCGG                 C^CGG
              893  CCGG                
NcoI         1250  CCATGG               C^CATGG
             1332  CCATGG              
NspI         1133  GCATGT               [GA]CATG^[CT]
             1208  GCATGC              
PflMI         201  CCACGAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1389  CCAGCCTGTGG         
PpuMI         862  GGGTCCC              [GA]G^G[AT]CC[CT]
              905  AGGACCT             
SmlI          472  CTCAAG               C^T[CT][GA]AG
              833  CTCGAG              
TaqI           76  TCGA                 T^CGA
              834  TCGA                
Tru9I         281  TTAA                 T^TAA
              673  TTAA                
TthHB8I        76  TCGA                 T^CGA
              834  TCGA                
Van91I        201  CCACGAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1389  CCAGCCTGTGG         
XcmI          482  CCAACAACCACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              873  CCAGTGTGAGAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BfaI        BmyI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HhaI        HinP1I      HinfI       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfcI        StyI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       
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