Restriction map for pF1KB5967
 (Linear) (Six-base) MAPSORT of: KIBB5967.seq  Check: 9598  from: 1  to: 1554



KIBB5967.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1429    1554 
   Size:       1429     125
 
AflIII A'CryG_T

Cuts at:      0    1196    1554 
   Size:       1196     358
 
AleI CACnn'nnGTG

Cuts at:      0    1240    1554 
   Size:       1240     314
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1089    1226    1554 
   Size:       1089     137     328

  Fragments arranged by size: 

                1089     328     137
 
ApaI G_GGCC'C

Cuts at:      0     102    1301    1554 
   Size:        102    1199     253

  Fragments arranged by size: 

                1199     253     102
 
ApaLI G'TGCA_C

Cuts at:      0     409    1513    1554 
   Size:        409    1104      41

  Fragments arranged by size: 

                1104     409      41
 
ApoI r'AATT_y

Cuts at:      0     196     343     519     956    1554 
   Size:        196     147     176     437     598

  Fragments arranged by size: 

                 598     437     196     176     147
 
AvaI C'yCGr_G

Cuts at:      0      82      94     833    1293    1554 
   Size:         82      12     739     460     261

  Fragments arranged by size: 

                 739     460     261      82      12
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     951     984    1554 
   Size:        951      33     570

  Fragments arranged by size: 

                 951     570      33
 
BanI G'GyrC_C

Cuts at:      0    1093    1438    1530    1554 
   Size:       1093     345      92      24

  Fragments arranged by size: 

                1093     345      92      24
 
BanII G_rGCy'C

Cuts at:      0     102     386     840    1301    1554 
   Size:        102     284     454     461     253

  Fragments arranged by size: 

                 461     454     284     253     102
 
BclI T'GATC_A

Cuts at:      0     221    1554 
   Size:        221    1333
 
BglI GCCn_nnn'nGGC

Cuts at:      0     433    1554 
   Size:        433    1121
 
BlpI GC'TnA_GC

Cuts at:      0     380    1554 
   Size:        380    1174
 
Bme1580I G_kGCm'C

Cuts at:      0     102     214     413    1172    1301    1517    1554 
   Size:        102     112     199     759     129     216      37

  Fragments arranged by size: 

                 759     216     199     129     112     102      37
 
BmgBI CAC'GTC

Cuts at:      0     612    1554 
   Size:        612     942
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     248    1034    1554 
   Size:        248     786     520

  Fragments arranged by size: 

                 786     520     248
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64    1554 
   Size:         64    1490
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     457    1554 
   Size:        457    1097
 
BsaAI yAC'GTr

Cuts at:      0     408    1199    1554 
   Size:        408     791     355

  Fragments arranged by size: 

                 791     408     355
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     403     433    1321    1351    1360    1390    1554 
   Size:        403      30     888      30       9      30     164

  Fragments arranged by size: 

                 888     403     164      30      30      30       9
 
BseYI C'CCAG_C

Cuts at:      0     103     140    1156    1171    1347    1554 
   Size:        103      37    1016      15     176     207

  Fragments arranged by size: 

                1016     207     176     103      37      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     588     715    1212    1264    1554 
   Size:        588     127     497      52     290

  Fragments arranged by size: 

                 588     497     290     127      52
 
BsiHKAI G_wGCw'C

Cuts at:      0     413     784     840    1517    1554 
   Size:        413     371      56     677      37

  Fragments arranged by size: 

                 677     413     371      56      37
 
BsmI GAATG_Cn'

Cuts at:      0     752    1554 
   Size:        752     802
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     462    1554 
   Size:        462    1092
 
Bsp1286I G_dGCh'C

Cuts at:      0     102     214     386     413     784     840    1172    1301 
   Size:        102     112     172      27     371      56     332     129

Cuts at:   1301    1517    1554 
   Size:        216      37

  Fragments arranged by size: 

                 371     332     216     172     129     112     102      56
                  37      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      71     330    1554 
   Size:         71     259    1224

  Fragments arranged by size: 

                1224     259      71
 
BsrBI CCG'CTC

Cuts at:      0    1064    1282    1554 
   Size:       1064     218     272

  Fragments arranged by size: 

                1064     272     218
 
BssHII G'CGCG_C

Cuts at:      0      89    1554 
   Size:         89    1465
 
BstBI TT'CG_AA

Cuts at:      0      76    1554 
   Size:         76    1478
 
BtgI C'CryG_G

Cuts at:      0     767    1250    1332    1379    1554 
   Size:        767     483      82      47     175

  Fragments arranged by size: 

                 767     483     175      82      47
 
BtsI GCAGTG_nn'

Cuts at:      0     644     720     934    1411    1554 
   Size:        644      76     214     477     143

  Fragments arranged by size: 

                 644     477     214     143      76
 
DraIII CAC_nnn'GTG

Cuts at:      0     207     555    1042    1512    1554 
   Size:        207     348     487     470      42

  Fragments arranged by size: 

                 487     470     348     207      42
 
EaeI y'GGCC_r

Cuts at:      0       2     396    1201    1325    1554 
   Size:          2     394     805     124     229

  Fragments arranged by size: 

                 805     394     229     124       2
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     258    1554 
   Size:        258    1296
 
EcoICRI GAG'CTC

Cuts at:      0     838    1554 
   Size:        838     716
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64    1429    1554 
   Size:         64    1365     125

  Fragments arranged by size: 

                1365     125      64
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1101    1554 
   Size:       1101     453
 
EcoO109I rG'GnC_Cy

Cuts at:      0      98      99     863     906    1086    1297    1298    1554 
   Size:         98       1     764      43     180     211       1     256

  Fragments arranged by size: 

                 764     256     211     180      98      43       1       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1229    1554 
   Size:       1229     325
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     536     568    1554 
   Size:        536      32     986

  Fragments arranged by size: 

                 986     536      32
 
HindIII A'AGCT_T

Cuts at:      0    1127    1554 
   Size:       1127     427
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     830    1554 
   Size:        830     724
 
MscI TGG'CCA

Cuts at:      0    1203    1327    1554 
   Size:       1203     124     227

  Fragments arranged by size: 

                1203     227     124
 
MslI CAynn'nnrTG

Cuts at:      0     217    1240    1554 
   Size:        217    1023     314

  Fragments arranged by size: 

                1023     314     217
 
MspA1I CmG'CkG

Cuts at:      0    1122    1190    1226    1347    1554 
   Size:       1122      68      36     121     207

  Fragments arranged by size: 

                1122     207     121      68      36
 
NcoI C'CATG_G

Cuts at:      0    1250    1332    1554 
   Size:       1250      82     222

  Fragments arranged by size: 

                1250     222      82
 
NspI r_CATG'y

Cuts at:      0    1137    1212    1554 
   Size:       1137      75     342

  Fragments arranged by size: 

                1137     342      75
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     207    1395    1554 
   Size:        207    1188     159

  Fragments arranged by size: 

                1188     207     159
 
PmlI CAC'GTG

Cuts at:      0    1199    1554 
   Size:       1199     355
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1345    1377    1554 
   Size:       1345      32     177

  Fragments arranged by size: 

                1345     177      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     863     906    1554 
   Size:        863      43     648

  Fragments arranged by size: 

                 863     648      43
 
PspOMI G'GGCC_C

Cuts at:      0      98    1297    1554 
   Size:         98    1199     257

  Fragments arranged by size: 

                1199     257      98
 
PstI C_TGCA'G

Cuts at:      0    1430    1554 
   Size:       1430     124
 
PvuII CAG'CTG

Cuts at:      0    1190    1226    1347    1554 
   Size:       1190      36     121     207

  Fragments arranged by size: 

                1190     207     121      36
 
SacI G_AGCT'C

Cuts at:      0     840    1554 
   Size:        840     714
 
SanDI GG'GwC_CC

Cuts at:      0     863    1554 
   Size:        863     691
 
SfcI C'TryA_G

Cuts at:      0    1101    1218    1426    1554 
   Size:       1101     117     208     128

  Fragments arranged by size: 

                1101     208     128     117
 
SmlI C'TyrA_G

Cuts at:      0     472     833    1554 
   Size:        472     361     721

  Fragments arranged by size: 

                 721     472     361
 
SphI G_CATG'C

Cuts at:      0    1212    1554 
   Size:       1212     342
 
StyI C'CwwG_G

Cuts at:      0     230    1250    1332    1480    1554 
   Size:        230    1020      82     148      74

  Fragments arranged by size: 

                1020     230     148      82      74
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     154     428     936    1053    1081    1554 
   Size:        154     274     508     117      28     473

  Fragments arranged by size: 

                 508     473     274     154     117      28
 
TatI w'GTAC_w

Cuts at:      0     296    1554 
   Size:        296    1258
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     149     181     403     435     990    1022    1360    1392 
   Size:        149      32     222      32     555      32     338      32

Cuts at:   1392    1554 
   Size:        162

  Fragments arranged by size: 

                 555     338     222     162     149      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1110    1554 
   Size:       1110     444
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     489     880    1554 
   Size:        489     391     674

  Fragments arranged by size: 

                 674     489     391
 
XhoI C'TCGA_G

Cuts at:      0     833    1554 
   Size:        833     721
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    AlwNI     ApaI    ApaLI     ApoI     AvaI
     BaeI     BanI    BanII     BclI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BpmI    BpuEI    BsaAI    BsaXI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspMI    BsrBI   BssHII    BstBI     BtgI
     BtsI   DraIII     EaeI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI    Hin4I  HindIII     MmeI     MscI     MslI   MspA1I     NcoI
     NspI    PflMI     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII
     SacI    SanDI     SfcI     SmlI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     AscI     AseI    AsiSI    AvrII    BamHI
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI    BglII     BmtI
     BplI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BseRI    BsiEI
    BsiWI    BspEI    BspHI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI     DrdI     EagI     EarI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PfoI     PmeI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     XbaI     XmaI     XmnI     ZraI
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