Restriction map for pF1KB5908
 (Linear) (Six-base) MAPSORT of: KIBB5908.seq  Check: 8430  from: 1  to: 1560



KIBB5908.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     321    1560 
   Size:        321    1239
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     905    1273    1560 
   Size:        115     790     368     287

  Fragments arranged by size: 

                 790     368     287     115
 
AflII C'TTAA_G

Cuts at:      0     349    1560 
   Size:        349    1211
 
AgeI A'CCGG_T

Cuts at:      0     510    1560 
   Size:        510    1050
 
AleI CACnn'nnGTG

Cuts at:      0     331    1560 
   Size:        331    1229
 
AlwNI CAG_nnn'CTG

Cuts at:      0     237     883    1560 
   Size:        237     646     677

  Fragments arranged by size: 

                 677     646     237
 
AvaI C'yCGr_G

Cuts at:      0     771    1178    1560 
   Size:        771     407     382

  Fragments arranged by size: 

                 771     407     382
 
BamHI G'GATC_C

Cuts at:      0     735    1414    1560 
   Size:        735     679     146

  Fragments arranged by size: 

                 735     679     146
 
BanI G'GyrC_C

Cuts at:      0     333     513    1339    1560 
   Size:        333     180     826     221

  Fragments arranged by size: 

                 826     333     221     180
 
BanII G_rGCy'C

Cuts at:      0      62     875    1185    1560 
   Size:         62     813     310     375

  Fragments arranged by size: 

                 813     375     310      62
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1433    1560 
   Size:       1433     127
 
Bme1580I G_kGCm'C

Cuts at:      0     272     308     628    1560 
   Size:        272      36     320     932

  Fragments arranged by size: 

                 932     320     272      36
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     243    1379    1560 
   Size:        243    1136     181

  Fragments arranged by size: 

                1136     243     181
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     135    1144    1208    1560 
   Size:        135    1009      64     352

  Fragments arranged by size: 

                1009     352     135      64
 
Bpu10I CC'TnA_GC

Cuts at:      0     466    1560 
   Size:        466    1094
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1285    1560 
   Size:       1285     275
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1219    1560 
   Size:       1219     341
 
BsaWI w'CCGG_w

Cuts at:      0     510    1560 
   Size:        510    1050
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1437    1467    1512    1542    1560 
   Size:       1437      30      45      30      18

  Fragments arranged by size: 

                1437      45      30      30      18
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58     138    1536    1560 
   Size:         58      80    1398      24

  Fragments arranged by size: 

                1398      80      58      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376    1560 
   Size:        376    1184
 
BsiHKAI G_wGCw'C

Cuts at:      0     875     897    1130    1185    1553    1560 
   Size:        875      22     233      55     368       7

  Fragments arranged by size: 

                 875     368     233      55      22       7
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1537    1560 
   Size:       1537      23
 
Bsp1286I G_dGCh'C

Cuts at:      0      62     272     308     628     875     897    1130    1185 
   Size:         62     210      36     320     247      22     233      55

Cuts at:   1185    1553    1560 
   Size:        368       7

  Fragments arranged by size: 

                 368     320     247     233     210      62      55      36
                  22       7
 
BspHI T'CATG_A

Cuts at:      0      51    1560 
   Size:         51    1509
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     321     402    1560 
   Size:        321      81    1158

  Fragments arranged by size: 

                1158     321      81
 
BsrFI r'CCGG_y

Cuts at:      0     510    1136    1560 
   Size:        510     626     424

  Fragments arranged by size: 

                 626     510     424
 
BsrGI T'GTAC_A

Cuts at:      0    1312    1560 
   Size:       1312     248
 
BssSI C'ACGA_G

Cuts at:      0     647    1560 
   Size:        647     913
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     189     237    1146    1560 
   Size:        189      48     909     414

  Fragments arranged by size: 

                 909     414     189      48
 
BstBI TT'CG_AA

Cuts at:      0      32    1560 
   Size:         32    1528
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1410    1560 
   Size:       1410     150
 
BstYI r'GATC_y

Cuts at:      0     735    1414    1560 
   Size:        735     679     146

  Fragments arranged by size: 

                 735     679     146
 
BtgI C'CryG_G

Cuts at:      0     542    1052    1208    1560 
   Size:        542     510     156     352

  Fragments arranged by size: 

                 542     510     352     156
 
ClaI AT'CG_AT

Cuts at:      0     680    1560 
   Size:        680     880
 
EaeI y'GGCC_r

Cuts at:      0     899    1560 
   Size:        899     661
 
EarI CTCTTCn'nnn_

Cuts at:      0      50      91     593    1535    1560 
   Size:         50      41     502     942      25

  Fragments arranged by size: 

                 942     502      50      41      25
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      99    1560 
   Size:         99    1461
 
EcoICRI GAG'CTC

Cuts at:      0     873    1183    1560 
   Size:        873     310     377

  Fragments arranged by size: 

                 873     377     310
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     135     905    1144    1208    1273    1560 
   Size:        115      20     770     239      64      65     287

  Fragments arranged by size: 

                 770     287     239     115      65      64      20
 
EcoO109I rG'GnC_Cy

Cuts at:      0     125    1560 
   Size:        125    1435
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1303    1560 
   Size:       1303     257
 
HincII GTy'rAC

Cuts at:      0      88    1560 
   Size:         88    1472
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     590    1560 
   Size:        590     970
 
MscI TGG'CCA

Cuts at:      0     901    1560 
   Size:        901     659
 
MslI CAynn'nnrTG

Cuts at:      0     331     547    1408    1435    1560 
   Size:        331     216     861      27     125

  Fragments arranged by size: 

                 861     331     216     125      27
 
MspA1I CmG'CkG

Cuts at:      0      93    1560 
   Size:         93    1467
 
NcoI C'CATG_G

Cuts at:      0     542    1560 
   Size:        542    1018
 
NspI r_CATG'y

Cuts at:      0     454    1020    1560 
   Size:        454     566     540

  Fragments arranged by size: 

                 566     540     454
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      90     122    1560 
   Size:         90      32    1438

  Fragments arranged by size: 

                1438      90      32
 
PsiI TTA'TAA

Cuts at:      0     590     710    1560 
   Size:        590     120     850

  Fragments arranged by size: 

                 850     590     120
 
PspXI vC'TCGA_Gb

Cuts at:      0    1178    1560 
   Size:       1178     382
 
PstI C_TGCA'G

Cuts at:      0     242    1276    1560 
   Size:        242    1034     284

  Fragments arranged by size: 

                1034     284     242
 
SacI G_AGCT'C

Cuts at:      0     875    1185    1560 
   Size:        875     310     375

  Fragments arranged by size: 

                 875     375     310
 
SapI GCTCTTCn'nnn_

Cuts at:      0      50      91     593    1560 
   Size:         50      41     502     967

  Fragments arranged by size: 

                 967     502      50      41
 
SfcI C'TryA_G

Cuts at:      0     238    1272    1392    1560 
   Size:        238    1034     120     168

  Fragments arranged by size: 

                1034     238     168     120
 
SgrAI Cr'CCGG_yG

Cuts at:      0     510    1560 
   Size:        510    1050
 
SmlI C'TyrA_G

Cuts at:      0     349    1178    1264    1560 
   Size:        349     829      86     296

  Fragments arranged by size: 

                 829     349     296      86
 
StuI AGG'CCT

Cuts at:      0     821    1560 
   Size:        821     739
 
StyI C'CwwG_G

Cuts at:      0     542     801    1560 
   Size:        542     259     759

  Fragments arranged by size: 

                 759     542     259
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     546    1560 
   Size:        546    1014
 
TatI w'GTAC_w

Cuts at:      0    1312    1560 
   Size:       1312     248
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     300     332    1437    1469    1560 
   Size:        300      32    1105      32      91

  Fragments arranged by size: 

                1105     300      91      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     540    1019    1560 
   Size:        540     479     541

  Fragments arranged by size: 

                 541     540     479
 
XhoI C'TCGA_G

Cuts at:      0    1178    1560 
   Size:       1178     382
 
XmnI GAAnn'nnTTC

Cuts at:      0    1259    1560 
   Size:       1259     301
 

 Enzymes that do cut:

     AarI     AcuI    AflII     AgeI     AleI    AlwNI     AvaI    BamHI
     BanI    BanII     BbsI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspHI    BspMI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI
    BstYI     BtgI     ClaI     EaeI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I     FalI   HincII     MmeI     MscI     MslI   MspA1I     NcoI
     NspI     PpiI     PsiI    PspXI     PstI     SacI     SapI     SfcI
    SgrAI     SmlI     StuI     StyI    TaqII     TatI     TstI     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AhdI     AlfI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI    BsaAI    BsaBI    BsaHI
    BseYI    BsiEI    BsiWI     BsmI    BspEI    BsrBI    BsrDI   BssHII
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI    CspCI     DraI   DraIII
     DrdI     EagI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI
    PshAI   PspOMI     PsrI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI
     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com