Restriction map for pF1KB5848
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbvCI       BfrI        BglII       
BlnI        BsaAI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmFI       BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BssHII      BssSI       
BstBI       BstEII      BstPI       BstUI       BstXI       
ClaI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoT14I     FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MroI        MunI        MvnI        NarI        
NcoI        NdeI        NlaIV       NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SmaI        SnaBI       SpeI        SphI        Sse8387I    
StyI        SwaI        Tth111I     Van91I      XhoI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   62 enzymes) #
###################################

AocI          699  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       107  AGCGCT               AGC^GCT
Asp700I      1132  GAATATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII          84  GGTCC                G^G[AT]CC
BalI          952  TGGCCA               TGG^CCA
BbsI          621  GAAGAC               GAAGAC(2/6)
BcgI         1280  GCAAGCTTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1078  GTATCC               GTATCC(6/5)
BcnI           74  CCCGG                CC^[GC]GG
BglI           40  GCCATCTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          868  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      868  GCTCAGC              GC^T[ACGT]AGC
BpuAI         621  GAAGAC               GAAGAC(2/6)
BsaBI         424  GATTTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI          667  GCATTC               GAATGC(1/-1)
BspMI         961  ACCTGC               ACCTGC(4/8)
BsrFI          18  GCCGGC               [GA]^CCGG[CT]
BstAPI        159  GCACCCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        441  GGATG                GGATG(2/0)
BstYI         597  AGATCC               [GA]^GATC[CT]
Bsu36I        699  CCTCAGG              CC^T[ACGT]AGG
CelII         868  GCTCAGC              GC^T[ACGT]AGC
Cfr10I         18  GCCGGC               [GA]^CCGG[CT]
Cfr13I         84  GGTCC                G^G[ACGT]CC
CpoI           83  CGGTCCG              CG^G[AT]CCG
EaeI          952  TGGCCA               [CT]^GGCC[GA]
Eco47III      107  AGCGCT               AGC^GCT
Eco81I        699  CCTCAGG              CC^T[ACGT]AGG
EcoRI         490  GAATTC               G^AATTC
EcoRV        1065  GATATC               GAT^ATC
EcoT22I       835  ATGCAT               ATGCA^T
FokI          441  GGATG                GGATG(9/13)
HindIII      1282  AAGCTT               A^AGCTT
MaeIII        611  GTGAC                ^GT[ACGT]AC
MamI          424  GATTTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          597  AGATCC               [GA]^GATC[CT]
MluNI         952  TGGCCA               TGG^CCA
MscI          952  TGGCCA               TGG^CCA
MslI          724  CATTTCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        163  CCGCTG               C[AC]G^C[GT]G
NaeI           18  GCCGGC               GCC^GGC
NciI           74  CCCGG                CC^[GC]GG
NgoMIV         18  GCCGGC               G^CCGGC
NheI          873  GCTAGC               G^CTAGC
NsiI          835  ATGCAT               ATGCA^T
NspI         1424  ACATGC               [GA]CATG^[CT]
PleI          256  GACTC                GAGTC(4/5)
PstI          963  CTGCAG               CTGCA^G
RsrII          83  CGGTCCG              CG^G[AT]CCG
Sau96I         84  GGTCC                G^G[ACGT]CC
SfaNI         181  GATGC                GCATC(5/9)
SfcI          963  CTGCAG               C^T[GA][CT]AG
SgrAI          17  CGCCGGCG             C[GA]^CCGG[CT]G
SspI          753  AATATT               AAT^ATT
StuI          458  AGGCCT               AGG^CCT
TaqI         1289  TCGA                 T^CGA
Tsp45I        611  GTGAC                ^GT[GC]AC
TthHB8I      1289  TCGA                 T^CGA
XbaI         1398  TCTAGA               T^CTAGA
XcmI         1229  CCATTGTAGCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         597  AGATCC               [GA]^GATC[CT]
XmnI         1132  GAATATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI          236  GTATAC               GT^[AC][GT]AC
             1243  GTATAC              
AlwI          430  GATCC                GGATC(4/5)
              598  GATCC               
AlwNI         571  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              892  CAGTTCCTG           
BanII         117  GGGCTC               G[GA]GC[CT]^C
              513  GAGCCC              
BmyI          117  GGGCTC               G[AGT]GC[ACT]^C
              513  GAGCCC              
BpmI          577  CTGGAG               CTGGAG(16/14)
              898  CTGGAG              
BseRI        1153  GAGGAG               GAGGAG(10/8)
             1375  GAGGAG              
BsgI          157  CTGCAC               GTGCAG(16/14)
              631  CTGCAC              
BsiYI          16  CCGCCGGCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               75  CCGGAAATCGG         
BslI           16  CCGCCGGCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               75  CCGGAAATCGG         
Bsp1286I      117  GGGCTC               G[AGT]GC[ACT]^C
              513  GAGCCC              
Bsp1407I      888  TGTACA               T^GTACA
             1196  TGTACA              
BsrDI         534  CATTGC               GCAATG(2/0)
              782  CATTGC              
BsrGI         888  TGTACA               T^GTACA
             1196  TGTACA              
Bst1107I      236  GTATAC               GTA^TAC
             1243  GTATAC              
BstZ17I       236  GTATAC               GTA^TAC
             1243  GTATAC              
FauI          162  CCCGC                CCCGC(4/6)
               22  GCGGG               
HaeII          34  AGCGCC               [GA]GCGC^[CT]
              107  AGCGCT              
HapII          19  CCGG                 C^CGG
               75  CCGG                
HpaII          19  CCGG                 C^CGG
               75  CCGG                
HphI          978  GGTGA                GGTGA(8/7)
               71  TCACC               
MaeII         609  ACGT                 A^CGT
             1350  ACGT                
MspI           19  CCGG                 C^CGG
               75  CCGG                
SapI          273  GAAGAGC              GCTCTTC(1/4)
             1045  GAAGAGC             
SmlI          454  CTTGAG               C^T[CT][GA]AG
              498  CTTGAG              
SspBI         888  TGTACA               T^GTACA
             1196  TGTACA              
TfiI          349  GAATC                G^A[AT]TC
             1221  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BbvI        BclI        BfaI        BsaJI       BsrI        
BssKI       BstNI       Cac8I       CfoI        DdeI        
DpnII       EarI        EcoRII      FbaI        Fnu4HI      
HaeIII      HhaI        HinP1I      HinfI       ItaI        
Ksp632I     MaeI        MboI        MboII       MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
RsaI        Sau3AI      ScrFI       Tru9I       TseI        
Tsp509I     TspRI       
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