Restriction map for pF1KB5848
 (Linear) (Six-base) MAPSORT of: KIBB5848.seq  Check: 4586  from: 1  to: 1458



KIBB5848.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AccI GT'mk_AC

Cuts at:      0     237    1244    1458 
   Size:        237    1007     214

  Fragments arranged by size: 

                1007     237     214
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640    1458 
   Size:        640     818
 
AfeI AGC'GCT

Cuts at:      0     109    1458 
   Size:        109    1349
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     599     631    1458 
   Size:        599      32     827

  Fragments arranged by size: 

                 827     599      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     576     897    1458 
   Size:        576     321     561

  Fragments arranged by size: 

                 576     561     321
 
ApoI r'AATT_y

Cuts at:      0     490     559     638     649     946    1169    1202    1458 
   Size:        490      69      79      11     297     223      33     256

  Fragments arranged by size: 

                 490     297     256     223      79      69      33      11
            
 
BanII G_rGCy'C

Cuts at:      0     121     517    1458 
   Size:        121     396     941

  Fragments arranged by size: 

                 941     396     121
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     628    1458 
   Size:        628     830
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1269    1303    1458 
   Size:       1269      34     155

  Fragments arranged by size: 

                1269     155      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1089    1458 
   Size:       1089     369
 
BclI T'GATC_A

Cuts at:      0     377     552     929    1458 
   Size:        377     175     377     529

  Fragments arranged by size: 

                 529     377     377     175
 
BfrBI ATG'CAT

Cuts at:      0     837    1458 
   Size:        837     621
 
BglI GCCn_nnn'nGGC

Cuts at:      0      46    1458 
   Size:         46    1412
 
BlpI GC'TnA_GC

Cuts at:      0     869    1458 
   Size:        869     589
 
BmtI G_CTAG'C

Cuts at:      0     877    1458 
   Size:        877     581
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598     919    1458 
   Size:        598     321     539

  Fragments arranged by size: 

                 598     539     321
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     475     519    1458 
   Size:        475      44     939

  Fragments arranged by size: 

                 939     475      44
 
BsaBI GATnn'nnATC

Cuts at:      0     428    1458 
   Size:        428    1030
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     390     420     557     587    1458 
   Size:        390      30     137      30     871

  Fragments arranged by size: 

                 871     390     137      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1168    1390    1458 
   Size:       1168     222      68

  Fragments arranged by size: 

                1168     222      68
 
BseYI C'CCAG_C

Cuts at:      0     170    1458 
   Size:        170    1288
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     616    1458 
   Size:        142     474     842

  Fragments arranged by size: 

                 842     474     142
 
BsmI GAATG_Cn'

Cuts at:      0     667    1458 
   Size:        667     791
 
Bsp1286I G_dGCh'C

Cuts at:      0     121     517    1458 
   Size:        121     396     941

  Fragments arranged by size: 

                 941     396     121
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     970    1458 
   Size:        970     488
 
BsrDI GCAATG_nn'

Cuts at:      0     533     781    1458 
   Size:        533     248     677

  Fragments arranged by size: 

                 677     533     248
 
BsrFI r'CCGG_y

Cuts at:      0      18    1458 
   Size:         18    1440
 
BsrGI T'GTAC_A

Cuts at:      0     888    1196    1458 
   Size:        888     308     262

  Fragments arranged by size: 

                 888     308     262
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     165    1458 
   Size:        165    1293
 
BstYI r'GATC_y

Cuts at:      0     597    1458 
   Size:        597     861
 
BstZ17I GTA'TAC

Cuts at:      0     238    1245    1458 
   Size:        238    1007     213

  Fragments arranged by size: 

                1007     238     213
 
Bsu36I CC'TnA_GG

Cuts at:      0     700    1458 
   Size:        700     758
 
BtsI GCAGTG_nn'

Cuts at:      0     235    1458 
   Size:        235    1223
 
EaeI y'GGCC_r

Cuts at:      0     952    1458 
   Size:        952     506
 
EarI CTCTTCn'nnn_

Cuts at:      0     268     431    1040    1458 
   Size:        268     163     609     418

  Fragments arranged by size: 

                 609     418     268     163
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598     640     919    1458 
   Size:        598      42     279     539

  Fragments arranged by size: 

                 598     539     279      42
 
EcoRI G'AATT_C

Cuts at:      0     490    1458 
   Size:        490     968
 
EcoRV GAT'ATC

Cuts at:      0    1067    1458 
   Size:       1067     391
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1069    1458 
   Size:       1069     389
 
HaeII r_GCGC'y

Cuts at:      0      38     111    1458 
   Size:         38      73    1347

  Fragments arranged by size: 

                1347      73      38
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     656     688    1458 
   Size:        656      32     770

  Fragments arranged by size: 

                 770     656      32
 
HindIII A'AGCT_T

Cuts at:      0    1282    1458 
   Size:       1282     176
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1249    1458 
   Size:       1249     209
 
MscI TGG'CCA

Cuts at:      0     954    1458 
   Size:        954     504
 
MslI CAynn'nnrTG

Cuts at:      0     728    1458 
   Size:        728     730
 
MspA1I CmG'CkG

Cuts at:      0     165    1458 
   Size:        165    1293
 
NaeI GCC'GGC

Cuts at:      0      20    1458 
   Size:         20    1438
 
NgoMIV G'CCGG_C

Cuts at:      0      18    1458 
   Size:         18    1440
 
NheI G'CTAG_C

Cuts at:      0     873    1458 
   Size:        873     585
 
NsiI A_TGCA'T

Cuts at:      0     839    1458 
   Size:        839     619
 
NspI r_CATG'y

Cuts at:      0    1428    1458 
   Size:       1428      30
 
PasI CC'CwG_GG

Cuts at:      0     114    1458 
   Size:        114    1344
 
PfoI T'CCnGG_A

Cuts at:      0     575     896    1458 
   Size:        575     321     562

  Fragments arranged by size: 

                 575     562     321
 
PstI C_TGCA'G

Cuts at:      0     967    1458 
   Size:        967     491
 
RsrII CG'GwC_CG

Cuts at:      0      84    1458 
   Size:         84    1374
 
SapI GCTCTTCn'nnn_

Cuts at:      0     268    1040    1458 
   Size:        268     772     418

  Fragments arranged by size: 

                 772     418     268
 
SfcI C'TryA_G

Cuts at:      0     963    1458 
   Size:        963     495
 
SgrAI Cr'CCGG_yG

Cuts at:      0      18    1458 
   Size:         18    1440
 
SmlI C'TyrA_G

Cuts at:      0     454     498    1458 
   Size:        454      44     960

  Fragments arranged by size: 

                 960     454      44
 
SspI AAT'ATT

Cuts at:      0     755    1458 
   Size:        755     703
 
StuI AGG'CCT

Cuts at:      0     460    1458 
   Size:        460     998
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      72    1458 
   Size:         72    1386
 
TatI w'GTAC_w

Cuts at:      0     888    1115    1196    1458 
   Size:        888     227      81     262

  Fragments arranged by size: 

                 888     262     227      81
 
XbaI T'CTAG_A

Cuts at:      0    1398    1458 
   Size:       1398      60
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1236    1458 
   Size:       1236     222
 
XmnI GAAnn'nnTTC

Cuts at:      0    1136    1458 
   Size:       1136     322
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     AloI    AlwNI     ApoI    BanII     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI     BmtI     BpmI
    BpuEI    BsaBI    BsaXI    BseRI    BseYI     BsgI     BsmI Bsp1286I
    BspMI    BsrDI    BsrFI    BsrGI   BstAPI    BstYI  BstZ17I   Bsu36I
     BtsI     EaeI     EarI  Eco57MI    EcoRI    EcoRV     FalI    HaeII
    Hin4I  HindIII     MmeI     MscI     MslI   MspA1I     NaeI   NgoMIV
     NheI     NsiI     NspI     PasI     PfoI     PstI    RsrII     SapI
     SfcI    SgrAI     SmlI     SspI     StuI    TaqII     TatI     XbaI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI    BglII Bme1580I
    BmgBI     BmrI     BplI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrBI   BssHII
    BssSI    BstBI   BstEII    BstXI     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI
     MluI     NarI     NcoI     NdeI     NotI     NruI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI     SmaI    SnaBI
     SpeI     SphI     SrfI     StyI     SwaI     TstI  Tth111I     XhoI
     XmaI     ZraI
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