Restriction map for pF1KB5842
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwNI       AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaII       AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbsI        
BbvCI       BciVI       BcnI        BfrI        BglI        
BglII       BlnI        BpuAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsmFI       BspDI       BspEI       BspLU11I    BspMI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoT14I     
EcoT22I     FauI        FseI        FspI        HapII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NgoMIV      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        StyI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Alw44I        646  GTGCAC               G^TGCAC
ApaLI         646  GTGCAC               G^TGCAC
AvaI         1184  CCCGAG               C^[CT]CG[GA]G
BanI          442  GGTGCC               G^G[CT][GA]CC
BbrPI         649  CACGTG               CAC^GTG
BcgI         1107  GCAGGAACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         1295  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     1295  GCTTAGC              GC^T[ACGT]AGC
BsaAI         649  CACGTG               [CT]AC^GT[GA]
BsaI         1246  GAGACC               GGTCTC(1/5)
BsgI          769  GTGCAG               GTGCAG(16/14)
BsmAI        1246  GAGAC                GTCTC(1/5)
BsmI          377  GCATTC               GAATGC(1/-1)
BsoBI        1184  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      849  TGTACA               T^GTACA
BsrBI         438  GAGCGG               CCGCTC(-3/-3)
BsrGI         849  TGTACA               T^GTACA
CelII        1295  GCTTAGC              GC^T[ACGT]AGC
CfoI          954  GCGC                 GCG^C
DraI          225  TTTAAA               TTT^AAA
DraII          66  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        649  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI            8  CGGCCA               [CT]^GGCC[GA]
EcoO109I       66  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         864  GAATTC               G^AATTC
EcoRV         403  GATATC               GAT^ATC
HaeII         953  AGCGCC               [GA]GCGC^[CT]
HhaI          954  GCGC                 GCG^C
HinP1I        954  GCGC                 G^CGC
MaeII         650  ACGT                 A^CGT
MwoI         1148  GCTTATGCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI         1047  GCTAGC               G^CTAGC
PmaCI         649  CACGTG               CAC^GTG
PmlI          649  CACGTG               CAC^GTG
ScaI          932  AGTACT               AGT^ACT
SexAI         201  ACCTGGT              A^CC[AT]GGT
SfaNI         551  GCATC                GCATC(5/9)
SspBI         849  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwI          243  GGATC                GGATC(4/5)
              681  GGATC               
AspHI           3  GAGCAC               G[AT]GC[AT]^C
              646  GTGCAC              
BbvI          168  GCAGC                GCAGC(8/12)
             1353  GCAGC               
BclI          711  TGATCA               T^GATCA
             1310  TGATCA              
BpmI          987  CTCCAG               CTGGAG(16/14)
               92  CTGGAG              
BsiHKAI         3  GAGCAC               G[AT]GC[AT]^C
              646  GTGCAC              
BspHI         860  TCATGA               T^CATGA
             1131  TCATGA              
BsrDI         384  CATTGC               GCAATG(2/0)
              499  GCAATG              
BstXI         581  CCAAAGTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1257  CCATCGTATTGG        
BstYI         243  GGATCT               [GA]^GATC[CT]
              681  GGATCT              
FbaI          711  TGATCA               T^GATCA
             1310  TGATCA              
HinfI         251  GAATC                G^A[ACGT]TC
              674  GAATC               
MflI          243  GGATCT               [GA]^GATC[CT]
              681  GGATCT              
NdeI          941  CATATG               CA^TATG
             1316  CATATG              
RcaI          860  TCATGA               T^CATGA
             1131  TCATGA              
SfcI          532  CTACAG               C^T[GA][CT]AG
             1430  CTGTAG              
TaqI         1009  TCGA                 T^CGA
             1116  TCGA                
TfiI          251  GAATC                G^A[AT]TC
              674  GAATC               
TseI          168  GCAGC                G^C[AT]GC
             1353  GCAGC               
Tsp45I        268  GTGAC                ^GT[GC]AC
              756  GTCAC               
TthHB8I      1009  TCGA                 T^CGA
             1116  TCGA                
XhoII         243  GGATCT               [GA]^GATC[CT]
              681  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BfaI        BmyI        BsaJI       BseRI       BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EcoNI       EcoRII      Fnu4HI      FokI        HaeIII      
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MspA1I      MvaI        
NdeII       NlaIII      NlaIV       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       Tru9I       Tsp509I     
TspRI       
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