Restriction map for pF1KB5836
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbsI        BcgI        BclI        BcnI        BglI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoRV       EcoT22I     FbaI        
FseI        FspI        HapII       HgaI        HindIII     
HpaII       KasI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MspI        MunI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII          15  GACGTC               GACGT^C
AcyI           15  GACGTC               G[GA]^CG[CT]C
AflII         291  CTTAAG               C^TTAAG
AluI           42  AGCT                 AG^CT
AlwI          184  GATCC                GGATC(4/5)
AseI          280  ATTAAT               AT^TAAT
AsnI          280  ATTAAT               AT^TAAT
AvaII         541  GGTCC                G^G[AT]CC
BalI          932  TGGCCA               TGG^CCA
BanII        1055  GGGCTC               G[GA]GC[CT]^C
BbvCI          43  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1210  GGATAC               GTATCC(6/5)
BfrI          291  CTTAAG               C^TTAAG
BglII         816  AGATCT               A^GATCT
BmyI         1055  GGGCTC               G[AGT]GC[ACT]^C
BpmI          134  CTGGAG               CTGGAG(16/14)
BsaHI          15  GACGTC               G[GA]^CG[CT]C
BsmAI        1026  GAGAC                GTCTC(1/5)
Bsp1286I     1055  GGGCTC               G[AGT]GC[ACT]^C
BsrBI         569  GAGCGG               CCGCTC(-3/-3)
BstEII        651  GGTGACC              G^GT[ACGT]ACC
BstPI         651  GGTGACC              G^GT[ACGT]ACC
BstXI         961  CCAGAGAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I        541  GGTCC                G^G[ACGT]CC
DraI          528  TTTAAA               TTT^AAA
DsaI          140  CCATGG               C^C[GA][CT]GG
EcoNI         775  CCTGTGGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       651  GGTGACC              G^GT[ACGT]ACC
FauI          571  GCGGG                CCCGC(4/6)
HaeII        1231  GGCGCT               [GA]GCGC^[CT]
Hin1I          15  GACGTC               G[GA]^CG[CT]C
HincII       1398  GTTAAC               GT[CT]^[GA]AC
HindII       1398  GTTAAC               GT[CT]^[GA]AC
HpaI         1398  GTTAAC               GTT^AAC
MaeII          16  ACGT                 A^CGT
MluNI         932  TGGCCA               TGG^CCA
MscI          932  TGGCCA               TGG^CCA
MspA1I         41  CAGCTG               C[AC]G^C[GT]G
NcoI          140  CCATGG               C^CATGG
PacI          364  TTAATTAA             TTAAT^TAA
PshAI        1306  GACCAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         280  ATTAAT               AT^TAAT
PstI           87  CTGCAG               CTGCA^G
PvuII          41  CAGCTG               CAG^CTG
Sau96I        541  GGTCC                G^G[ACGT]CC
ScaI         1358  AGTACT               AGT^ACT
SfcI           87  CTGCAG               C^T[GA][CT]AG
SspI         1046  AATATT               AAT^ATT
StuI         1062  AGGCCT               AGG^CCT
TaqI         1314  TCGA                 T^CGA
TthHB8I      1314  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccII          12  CGCG                 CG^CG
               27  CGCG                
AfaI         1154  GTAC                 GT^AC
             1359  GTAC                
BbvI         1329  GCAGC                GCAGC(8/12)
              850  GCTGC               
BsmFI         426  GTCCC                GGGAC(10/14)
              958  GTCCC               
BsrI          854  CCAGT                ACTGG(1/-1)
             1320  CCAGT               
BssKI          31  CCTGG                ^CC[ACGT]GG
             1246  CCTGG               
BstNI          31  CCTGG                CC^[AT]GG
             1246  CCTGG               
BstUI          12  CGCG                 CG^CG
               27  CGCG                
BstYI         183  AGATCC               [GA]^GATC[CT]
              816  AGATCT              
CfoI           28  GCGC                 GCG^C
             1232  GCGC                
EaeI          101  TGGCCG               [CT]^GGCC[GA]
              932  TGGCCA              
EarI          346  GAAGAG               CTCTTC(1/4)
             1192  GAAGAG              
EcoRI         399  GAATTC               G^AATTC
             1078  GAATTC              
EcoRII         31  CCTGG                ^CC[AT]GG
             1246  CCTGG               
HhaI           28  GCGC                 GCG^C
             1232  GCGC                
HinP1I         28  GCGC                 G^CGC
             1232  GCGC                
Ksp632I       346  GAAGAG               CTCTTC(1/4)
             1192  GAAGAG              
MflI          183  AGATCC               [GA]^GATC[CT]
              816  AGATCT              
MvaI           31  CCTGG                CC^[AT]GG
             1246  CCTGG               
MvnI           12  CGCG                 CG^CG
               27  CGCG                
MwoI            4  GCGACCTCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               21  GCCGCACGCGC         
NlaIII        141  CATG                 CATG^
             1198  CATG                
NlaIV         136  GGAGCC               GG[ACGT]^[ACGT]CC
              971  GGAACC              
RsaI         1154  GTAC                 GT^AC
             1359  GTAC                
SapI          346  GAAGAGC              GCTCTTC(1/4)
             1192  GAAGAGC             
ScrFI          31  CCTGG                CC^[ACGT]GG
             1246  CCTGG               
SmlI          240  CTCAAG               C^T[CT][GA]AG
              291  CTTAAG              
TseI          850  GCTGC                G^C[AT]GC
             1329  GCAGC               
Tsp45I        652  GTGAC                ^GT[GC]AC
             1007  GTCAC               
TspRI         698  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1073  CAGTG               
XhoII         183  AGATCC               [GA]^GATC[CT]
              816  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AcsI        ApoI        Asp700I     BfaI        
BsaJI       BsiYI       BslI        BstF5I      Cac8I       
DdeI        DpnII       EcoT14I     Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MslI        NdeII       Sau3AI      SfaNI       StyI        
TfiI        Tru9I       Tsp509I     XmnI        
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