Restriction map for pF1KB5824
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BcnI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        Bsp1407I    BspEI       BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      CpoI        DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        KasI        KpnI        KspI        
MaeII       MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NciI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI          136  GTAGAC               GT^[AC][GT]AC
AflIII       1042  ACATGT               A^C[GA][CT]GT
Asp700I       400  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          827  CCCGAG               C^[CT]CG[GA]G
BamHI        1090  GGATCC               G^GATCC
BbsI           19  GAAGAC               GAAGAC(2/6)
BciVI         984  GGATAC               GTATCC(6/5)
BclI         1050  TGATCA               T^GATCA
BglII         164  AGATCT               A^GATCT
BmyI            7  GTGCCC               G[AGT]GC[ACT]^C
BpuAI          19  GAAGAC               GAAGAC(2/6)
BsaBI         165  GATCTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI         827  CCCGAG               C^[CT]CG[GA]G
Bsp1286I        7  GTGCCC               G[AGT]GC[ACT]^C
BspDI         700  ATCGAT               AT^CGAT
BspHI         189  TCATGA               T^CATGA
BspLU11I     1042  ACATGT               A^CATGT
BsrDI         598  GCAATG               GCAATG(2/0)
ClaI          700  ATCGAT               AT^CGAT
DraII         823  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI          619  CCATGG               C^C[GA][CT]GG
EcoO109I      823  AGGCCCC              [GA]G^G[ACGT]CC[CT]
FauI          962  CCCGC                CCCGC(4/6)
FbaI         1050  TGATCA               T^GATCA
HaeIII        824  GGCC                 GG^CC
HapII        1323  CCGG                 C^CGG
HincII       1315  GTTGAC               GT[CT]^[GA]AC
HindII       1315  GTTGAC               GT[CT]^[GA]AC
HpaII        1323  CCGG                 C^CGG
HphI         1336  GGTGA                GGTGA(8/7)
MamI          165  GATCTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI         1323  CCGG                 C^CGG
NcoI          619  CCATGG               C^CATGG
PshAI         637  GACAGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         639  CAGCTG               CAG^CTG
RcaI          189  TCATGA               T^CATGA
SfcI          258  CTGTAG               C^T[GA][CT]AG
SmlI          805  CTCAAG               C^T[CT][GA]AG
SphI          302  GCATGC               GCATG^C
TaqI          701  TCGA                 T^CGA
TthHB8I       701  TCGA                 T^CGA
XcmI          562  CCAGTTGATGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          400  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AciI          963  CCGC                 CCGC(-3/-1)
             1113  GCGG                
AvaII         575  GGTCC                G^G[AT]CC
              624  GGACC               
BanI           52  GGTGCC               G^G[CT][GA]CC
             1081  GGCACC              
BfaI          715  CTAG                 C^TAG
              920  CTAG                
BseRI         710  CTCCTC               GAGGAG(10/8)
              266  GAGGAG              
BsmFI          26  GGGAC                GGGAC(10/14)
              623  GGGAC               
BstYI         164  AGATCT               [GA]^GATC[CT]
             1090  GGATCC              
EarI          537  GAAGAG               CTCTTC(1/4)
              542  GAAGAG              
EcoT14I       619  CCATGG               C^C[AT][AT]GG
              688  CCTTGG              
Ksp632I       537  GAAGAG               CTCTTC(1/4)
              542  GAAGAG              
MaeI          715  CTAG                 C^TAG
              920  CTAG                
MflI          164  AGATCT               [GA]^GATC[CT]
             1090  GGATCC              
MslI          190  CATGAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              297  CATAGGCATG          
MspA1I        639  CAGCTG               C[AC]G^C[GT]G
             1111  CAGCGG              
MwoI          598  GCAATGCTCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              709  GCTCCTCTAGC         
NdeI          515  CATATG               CA^TATG
             1054  CATATG              
NspI          302  GCATGC               [GA]CATG^[CT]
             1042  ACATGT              
PleI          830  GAGTC                GAGTC(4/5)
              853  GAGTC               
SfaNI         316  GATGC                GCATC(5/9)
             1153  GATGC               
StyI          619  CCATGG               C^C[AT][AT]GG
              688  CCTTGG              
XhoII         164  AGATCT               [GA]^GATC[CT]
             1090  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
BbvI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HinfI       ItaI        MaeIII      
MboI        MboII       MnlI        MseI        MvaI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScaI        ScrFI       SspI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
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