Restriction map for pF1KB5817
 (Circular) (Six-base) MAPSORT of: pF1KB5817.seq  Check: 3561  from: 1  to: 3749



pF1KB5817  3749 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AccI GT'mk_AC

Cuts at:    787     787 
   Size:       3749
 
Acc65I G'GTAC_C

Cuts at:    765     765 
   Size:       3749
 
AclI AA'CG_TT

Cuts at:   3589    3589 
   Size:       3749
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    232     613    1574    2006    2753    3021     232 
   Size:        381     961     432     747     268     960

  Fragments arranged by size: 

                 961     960     747     432     381     268
 
AflIII A'CryG_T

Cuts at:    521    1101    2205    3182     521 
   Size:        580    1104     977    1088

  Fragments arranged by size: 

                1104    1088     977     580
 
AgeI A'CCGG_T

Cuts at:   2137    2137 
   Size:       3749
 
AlwNI CAG_nnn'CTG

Cuts at:   1054    2621    1054 
   Size:       1567    2182
 
ApaLI G'TGCA_C

Cuts at:   2519    2519 
   Size:       3749
 
ApoI r'AATT_y

Cuts at:    461     753    3102    3358     461 
   Size:        292    2349     256     852

  Fragments arranged by size: 

                2349     852     292     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3749
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3749
 
AvaI C'yCGr_G

Cuts at:    769     769 
   Size:       3749
 
BamHI G'GATC_C

Cuts at:    630     774     630 
   Size:        144    3605
 
BanI G'GyrC_C

Cuts at:    765    1179    1427    1462     765 
   Size:        414     248      35    3052

  Fragments arranged by size: 

                3052     414     248      35
 
BanII G_rGCy'C

Cuts at:    741     763    1793     741 
   Size:         22    1030    2697

  Fragments arranged by size: 

                2697    1030      22
 
BbeI G_GCGC'C

Cuts at:   1183    1431    1183 
   Size:        248    3501
 
BbsI GAAGACnn'nnnn_

Cuts at:    437     437 
   Size:       3749
 
BciVI GTATCCnnnnn_n'

Cuts at:   1647    2408    3749    1647 
   Size:        761    1341    1647

  Fragments arranged by size: 

                1647    1341     761
 
BclI T'GATC_A

Cuts at:   1269    1269 
   Size:       3749
 
BglII A'GATC_T

Cuts at:   1264    3301    1264 
   Size:       2037    1712
 
BlpI GC'TnA_GC

Cuts at:    861     861 
   Size:       3749
 
Bme1580I G_kGCm'C

Cuts at:   1374    1467    2523    1374 
   Size:         93    1056    2600

  Fragments arranged by size: 

                2600    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    961    1042    1377    3521     961 
   Size:         81     335    2144    1189

  Fragments arranged by size: 

                2144    1189     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2116    2116 
   Size:       3749
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    391     727     742     391 
   Size:        336      15    3398

  Fragments arranged by size: 

                3398     336      15
 
Bpu10I CC'TnA_GC

Cuts at:    646     646 
   Size:       3749
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    925    1804    2296    2594     925 
   Size:        879     492     298    2080

  Fragments arranged by size: 

                2080     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3453    3453 
   Size:       3749
 
BsaAI yAC'GTr

Cuts at:    524    1102     524 
   Size:        578    3171
 
BsaBI GATnn'nnATC

Cuts at:      6    1263    3300       6 
   Size:       1257    2037     455

  Fragments arranged by size: 

                2037    1257     455
 
BsaHI Gr'CG_yC

Cuts at:   1180    1428    2130    3626    1180 
   Size:        248     702    1496    1303

  Fragments arranged by size: 

                1496    1303     702     248
 
BsaWI w'CCGG_w

Cuts at:    938    1459    2137    2411    2558    2969     938 
   Size:        521     678     274     147     411    1718

  Fragments arranged by size: 

                1718     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    364     394     364 
   Size:         30    3719
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    508     508 
   Size:       3749
 
BseYI C'CCAG_C

Cuts at:    318    1174    1932    2509    3005     318 
   Size:        856     758     577     496    1062

  Fragments arranged by size: 

                1062     856     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    652     652 
   Size:       3749
 
BsiEI CG_ry'CG

Cuts at:    104    1291    1337    2545     104 
   Size:       1187      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1187      46
 
BsiHKAI G_wGCw'C

Cuts at:    741     763    1541    1731    2523     741 
   Size:         22     778     190     792    1967

  Fragments arranged by size: 

                1967     792     778     190      22
 
Bsp1286I G_dGCh'C

Cuts at:    741     763    1374    1467    1541    1731    1793    2523     741 
   Size:         22     611      93      74     190      62     730    1967

  Fragments arranged by size: 

                1967     730     611     190      93      74      62      22
            
 
BspEI T'CCGG_A

Cuts at:   2969    2969 
   Size:       3749
 
BspHI T'CATG_A

Cuts at:   2925    2925 
   Size:       3749
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    226     799    1013    1315    1696     226 
   Size:        573     214     302     381    2279

  Fragments arranged by size: 

                2279     573     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2041    2095    3579    3744      50 
   Size:       1991      54    1484     165      55

  Fragments arranged by size: 

                1991    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1661    1661 
   Size:       3749
 
BsrFI r'CCGG_y

Cuts at:   1747    1928    2137    1747 
   Size:        181     209    3359

  Fragments arranged by size: 

                3359     209     181
 
BsrGI T'GTAC_A

Cuts at:    572     572 
   Size:       3749
 
BssSI C'ACGA_G

Cuts at:   2020    2378    2020 
   Size:        358    3391
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1126    1126 
   Size:       3749
 
BstBI TT'CG_AA

Cuts at:   2110    2110 
   Size:       3749
 
BstYI r'GATC_y

Cuts at:      1     630     774    1241    1264    1599    1845    2857    2943 
   Size:        629     144     467      23     335     246    1012      86

Cuts at:   2943    2955    3301       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     629     467     449     346     335     246     144
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3749
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1699    1880    2043    1699 
   Size:        181     163    3405

  Fragments arranged by size: 

                3405     181     163
 
BtsI GCAGTG_nn'

Cuts at:    245     992     245 
   Size:        747    3002
 
DraI TTT'AAA

Cuts at:    748     748 
   Size:       3749
 
DrdI GACnn_nn'nnGTC

Cuts at:   1455    2313    1455 
   Size:        858    2891
 
EaeI y'GGCC_r

Cuts at:    605    1334    1508    1899    1926    3271     605 
   Size:        729     174     391      27    1345    1083

  Fragments arranged by size: 

                1345    1083     729     391     174      27
 
EagI C'GGCC_G

Cuts at:   1334    1334 
   Size:       3749
 
EarI CTCTTCn'nnn_

Cuts at:   1772    1982    1772 
   Size:        210    3539
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1957    2267    2413    3559    1957 
   Size:        310     146    1146    2147

  Fragments arranged by size: 

                2147    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    739     761     739 
   Size:         22    3727
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    232     391     613     727     742    1574    2006    2753    3021 
   Size:        159     222     114      15     832     432     747     268

Cuts at:   3021     232 
   Size:        960

  Fragments arranged by size: 

                 960     832     747     432     268     222     159     114
                  15
 
EcoO109I rG'GnC_Cy

Cuts at:    888     888 
   Size:       3749
 
EcoRI G'AATT_C

Cuts at:    753     753 
   Size:       3749
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1768    1768 
   Size:       3749
 
FspI TGC'GCA

Cuts at:   1530    1530 
   Size:       3749
 
HaeII r_GCGC'y

Cuts at:   1183    1431    2453    3432    1183 
   Size:        248    1022     979    1500

  Fragments arranged by size: 

                1500    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1591    1621    1623    1653    1591 
   Size:         30       2      30    3687

  Fragments arranged by size: 

                3687      30      30       2
 
HincII GTy'rAC

Cuts at:    413     788     413 
   Size:        375    3374
 
KasI G'GCGC_C

Cuts at:   1179    1427    1179 
   Size:        248    3501
 
KpnI G_GTAC'C

Cuts at:    769     769 
   Size:       3749
 
MluI A'CGCG_T

Cuts at:   3182    3182 
   Size:       3749
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2420    2604    2420 
   Size:        184    3565
 
MscI TGG'CCA

Cuts at:    607    1510     607 
   Size:        903    2846
 
MslI CAynn'nnrTG

Cuts at:    542    1738    1865     542 
   Size:       1196     127    2426

  Fragments arranged by size: 

                2426    1196     127
 
MspA1I CmG'CkG

Cuts at:    860    1174    1534    2547    2792     860 
   Size:        314     360    1013     245    1817

  Fragments arranged by size: 

                1817    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1930    1930 
   Size:       3749
 
NarI GG'CG_CC

Cuts at:   1180    1428    1180 
   Size:        248    3501
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3749
 
NgoMIV G'CCGG_C

Cuts at:   1928    1928 
   Size:       3749
 
NspI r_CATG'y

Cuts at:    717     802    1302    2209     717 
   Size:         85     500     907    2257

  Fragments arranged by size: 

                2257     907     500      85
 
PciI A'CATG_T

Cuts at:   2205    2205 
   Size:       3749
 
PmeI GTTT'AAAC

Cuts at:    748     748 
   Size:       3749
 
PmlI CAC'GTG

Cuts at:    524     524 
   Size:       3749
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2888    2920    2888 
   Size:         32    3717
 
PstI C_TGCA'G

Cuts at:    395     796    1481     395 
   Size:        401     685    2663

  Fragments arranged by size: 

                2663     685     401
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3749
 
PvuII CAG'CTG

Cuts at:   1174    1534    1174 
   Size:        360    3389
 
RsrII CG'GwC_CG

Cuts at:   1944    1944 
   Size:       3749
 
SacI G_AGCT'C

Cuts at:    741     763     741 
   Size:         22    3727
 
SalI G'TCGA_C

Cuts at:    786     786 
   Size:       3749
 
SapI GCTCTTCn'nnn_

Cuts at:   1772    1982    1772 
   Size:        210    3539
 
SbfI CC_TGCA'GG

Cuts at:    796     796 
   Size:       3749
 
ScaI AGT'ACT

Cuts at:   3266    3266 
   Size:       3749
 
SfcI C'TryA_G

Cuts at:     33     391     792    1477    2470    2661      33 
   Size:        358     401     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     401     358     191
 
SfoI GGC'GCC

Cuts at:   1181    1429    1181 
   Size:        248    3501
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3749
 
SmaI CCC'GGG

Cuts at:    771     771 
   Size:       3749
 
SmlI C'TyrA_G

Cuts at:    904    1819    2311    2573     904 
   Size:        915     492     262    2080

  Fragments arranged by size: 

                2080     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3749
 
SphI G_CATG'C

Cuts at:    717     802    1302     717 
   Size:         85     500    3164

  Fragments arranged by size: 

                3164     500      85
 
SspI AAT'ATT

Cuts at:    258     258 
   Size:       3749
 
StyI C'CwwG_G

Cuts at:    107     401     455     608     883    1237     107 
   Size:        294      54     153     275     354    2619

  Fragments arranged by size: 

                2619     354     294     275     153      54
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1332    1465    1924    2133    1332 
   Size:        133     459     209    2948

  Fragments arranged by size: 

                2948     459     209     133
 
TatI w'GTAC_w

Cuts at:    572    3264     572 
   Size:       2692    1057
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    364     396     615     647    3266    3298     364 
   Size:         32     219      32    2619      32     815

  Fragments arranged by size: 

                2619     815     219      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1546    1546 
   Size:       3749
 
XbaI T'CTAG_A

Cuts at:    780     780 
   Size:       3749
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1244    1244 
   Size:       3749
 
XmaI C'CCGG_G

Cuts at:    769     769 
   Size:       3749
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI     PmeI     PmlI     PpiI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     SspI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI    BsiWI     BsmI    BsmBI   BssHII   BstEII    BstXI
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     StuI     SwaI     XhoI     XmnI     ZraI
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