Restriction map for pF1KB7942
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AvaII       AviII       AvrII       BamHI       
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BseRI       BsiHKAI     
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr13I      ClaI        CpoI        DpnII       
DraI        DraII       DraIII      DrdI        Eam1105I    
Eco47III    Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MamI        MboI        MfeI        MflI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NdeI        NdeII       NgoMIV      NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        Sau3AI      
Sau96I      ScaI        SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
SspBI       SspI        StuI        SwaI        TfiI        
Tsp509I     TspRI       Van91I      XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AflIII        622  ACATGT               A^C[GA][CT]GT
AspI          582  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           63  CCCGGG               C^[CT]CG[GA]G
BalI          659  TGGCCA               TGG^CCA
BanI          720  GGTGCC               G^G[CT][GA]CC
BbsI          373  GAAGAC               GAAGAC(2/6)
BmyI           16  GGGCAC               G[AGT]GC[ACT]^C
BpuAI         373  GAAGAC               GAAGAC(2/6)
BsaI          555  GAGACC               GGTCTC(1/5)
BsiEI          48  CGGCCG               CG[GA][CT]^CG
BsoBI          63  CCCGGG               C^[CT]CG[GA]G
Bsp1286I       16  GGGCAC               G[AGT]GC[ACT]^C
BspLU11I      622  ACATGT               A^CATGT
BsrDI         669  CATTGC               GCAATG(2/0)
BsrFI          36  ACCGGC               [GA]^CCGG[CT]
BstF5I        159  GGATG                GGATG(2/0)
Cfr10I         36  ACCGGC               [GA]^CCGG[CT]
DsaI          602  CCATGG               C^C[GA][CT]GG
EagI           48  CGGCCG               C^GGCCG
EclXI          48  CGGCCG               C^GGCCG
Eco52I         48  CGGCCG               C^GGCCG
EcoNI         396  CCTCACCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI          159  GGATG                GGATG(9/13)
HgaI          586  GCGTC                GACGC(5/10)
HincII        730  GTCAAC               GT[CT]^[GA]AC
HindII        730  GTCAAC               GT[CT]^[GA]AC
MluNI         659  TGGCCA               TGG^CCA
MscI          659  TGGCCA               TGG^CCA
MseI          494  TTAA                 T^TAA
MslI          651  CATCAACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          602  CCATGG               C^CATGG
NheI          468  GCTAGC               G^CTAGC
NotI           47  GCGGCCGC             GC^GGCCGC
NspI          622  ACATGT               [GA]CATG^[CT]
PflFI         582  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SapI          434  GAAGAGC              GCTCTTC(1/4)
SbfI           81  CCTGCAGG             CCTGCA^GG
SexAI         716  ACCAGGT              A^CC[AT]GGT
SmaI           63  CCCGGG               CCC^GGG
Sse8387I       81  CCTGCAGG             CCTGCA^GG
TaqI           74  TCGA                 T^CGA
Tru9I         494  TTAA                 T^TAA
Tth111I       582  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I        74  TCGA                 T^CGA
XmaCI          63  CCCGGG               C^CCGGG
XmaI           63  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI          538  GTAC                 GT^AC
              594  GTAC                
BfaI          118  CTAG                 C^TAG
              469  CTAG                
BsgI          641  CTGCAC               GTGCAG(16/14)
              412  GTGCAG              
BsmBI         587  CGTCTC               CGTCTC(1/5)
              509  GAGACG              
BspMI         113  ACCTGC               ACCTGC(4/8)
              414  GCAGGT              
BsrBI          28  GAGCGG               CCGCTC(-3/-3)
              340  GAGCGG              
CfoI           40  GCGC                 GCG^C
              599  GCGC                
EarI          270  GAAGAG               CTCTTC(1/4)
              434  GAAGAG              
EcoT14I       602  CCATGG               C^C[AT][AT]GG
              692  CCAAGG              
FauI          228  CCCGC                CCCGC(4/6)
              383  CCCGC               
HaeII          39  GGCGCT               [GA]GCGC^[CT]
              598  AGCGCC              
HhaI           40  GCGC                 GCG^C
              599  GCGC                
HinP1I         40  GCGC                 G^CGC
              599  GCGC                
HinfI         196  GAGTC                G^A[ACGT]TC
              206  GACTC               
Ksp632I       270  GAAGAG               CTCTTC(1/4)
              434  GAAGAG              
MaeI          118  CTAG                 C^TAG
              469  CTAG                
MaeIII        110  GTCAC                ^GT[ACGT]AC
              514  GTGAC               
PleI          206  GACTC                GAGTC(4/5)
              196  GAGTC               
PstI           82  CTGCAG               CTGCA^G
              316  CTGCAG              
PvuII         247  CAGCTG               CAG^CTG
              313  CAGCTG              
RsaI          538  GTAC                 GT^AC
              594  GTAC                
SfaNI         358  GATGC                GCATC(5/9)
              650  GCATC               
SfcI           82  CTGCAG               C^T[GA][CT]AG
              316  CTGCAG              
StyI          602  CCATGG               C^C[AT][AT]GG
              692  CCAAGG              
Tsp45I        110  GTCAC                ^GT[GC]AC
              514  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AluI        BbvI        BcnI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
BssKI       BstNI       Cac8I       DdeI        EaeI        
EcoRII      Fnu4HI      HaeIII      HapII       HpaII       
HphI        ItaI        MaeII       MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NlaIII      NlaIV       ScrFI       TseI        
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