Restriction map for
pF1KB7930
(Circular) (Six-base) MAPSORT of: pF1KB7930.seq Check: 8941 from: 1 to: 3752
pF1KB7930 3752 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 790 790
Size: 3752
Acc65I G'GTAC_C
Cuts at: 768 768
Size: 3752
AclI AA'CG_TT
Cuts at: 3592 3592
Size: 3752
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 1577 2009 2756 3024 460
Size: 1117 432 747 268 1188
Fragments arranged by size:
1188 1117 747 432 268
AflIII A'CryG_T
Cuts at: 1104 2208 3185 1104
Size: 1104 977 1671
Fragments arranged by size:
1671 1104 977
AgeI A'CCGG_T
Cuts at: 2140 2140
Size: 3752
AlwNI CAG_nnn'CTG
Cuts at: 1057 2624 1057
Size: 1567 2185
ApaLI G'TGCA_C
Cuts at: 2522 2522
Size: 3752
ApoI r'AATT_y
Cuts at: 756 3105 3361 756
Size: 2349 256 1147
Fragments arranged by size:
2349 1147 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3752
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
AvaI C'yCGr_G
Cuts at: 772 772
Size: 3752
BamHI G'GATC_C
Cuts at: 777 777
Size: 3752
BanI G'GyrC_C
Cuts at: 768 1182 1430 1465 768
Size: 414 248 35 3055
Fragments arranged by size:
3055 414 248 35
BanII G_rGCy'C
Cuts at: 766 1796 766
Size: 1030 2722
BbeI G_GCGC'C
Cuts at: 1186 1434 1186
Size: 248 3504
BciVI GTATCCnnnnn_n'
Cuts at: 522 1650 2411 3752 522
Size: 1128 761 1341 522
Fragments arranged by size:
1341 1128 761 522
BclI T'GATC_A
Cuts at: 1272 1272
Size: 3752
BglII A'GATC_T
Cuts at: 381 1267 3304 381
Size: 886 2037 829
Fragments arranged by size:
2037 886 829
BlpI GC'TnA_GC
Cuts at: 864 864
Size: 3752
Bme1580I G_kGCm'C
Cuts at: 341 1377 1470 2526 341
Size: 1036 93 1056 1567
Fragments arranged by size:
1567 1056 1036 93
BmrI ACTGGGnnnn_n'
Cuts at: 191 964 1045 1380 3524 191
Size: 773 81 335 2144 419
Fragments arranged by size:
2144 773 419 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 3752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 409 269
Size: 140 3612
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 928 1807 2299 2597 580
Size: 348 879 492 298 1735
Fragments arranged by size:
1735 879 492 348 298
BsaI GGTCTCn'nnnn_
Cuts at: 3456 3456
Size: 3752
BsaAI yAC'GTr
Cuts at: 1105 1105
Size: 3752
BsaBI GATnn'nnATC
Cuts at: 6 1266 3303 6
Size: 1260 2037 455
Fragments arranged by size:
2037 1260 455
BsaHI Gr'CG_yC
Cuts at: 1183 1431 2133 3629 1183
Size: 248 702 1496 1306
Fragments arranged by size:
1496 1306 702 248
BsaWI w'CCGG_w
Cuts at: 941 1462 2140 2414 2561 2972 941
Size: 521 678 274 147 411 1721
Fragments arranged by size:
1721 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1177 1935 2512 3008 1177
Size: 758 577 496 1921
Fragments arranged by size:
1921 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 133
Size: 3752
BsiEI CG_ry'CG
Cuts at: 104 1294 1340 2548 104
Size: 1190 46 1208 1308
Fragments arranged by size:
1308 1208 1190 46
BsiHKAI G_wGCw'C
Cuts at: 278 766 1544 1734 2526 278
Size: 488 778 190 792 1504
Fragments arranged by size:
1504 792 778 488 190
BsmI GAATG_Cn'
Cuts at: 336 336
Size: 3752
Bsp1286I G_dGCh'C
Cuts at: 278 341 766 1377 1470 1544 1734 1796 2526
Size: 63 425 611 93 74 190 62 730
Cuts at: 2526 278
Size: 1504
Fragments arranged by size:
1504 730 611 425 190 93 74 63
62
BspEI T'CCGG_A
Cuts at: 2972 2972
Size: 3752
BspHI T'CATG_A
Cuts at: 2928 2928
Size: 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 802 1016 1318 1699 802
Size: 214 302 381 2855
Fragments arranged by size:
2855 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2044 2098 3582 3747 50
Size: 1994 54 1484 165 55
Fragments arranged by size:
1994 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1664 1664
Size: 3752
BsrFI r'CCGG_y
Cuts at: 1750 1931 2140 1750
Size: 181 209 3362
Fragments arranged by size:
3362 209 181
BssSI C'ACGA_G
Cuts at: 2023 2381 2023
Size: 358 3394
BstAPI GCAn_nnn'nTGC
Cuts at: 1129 1129
Size: 3752
BstBI TT'CG_AA
Cuts at: 2113 2113
Size: 3752
BstYI r'GATC_y
Cuts at: 1 381 777 1244 1267 1602 1848 2860 2946
Size: 380 396 467 23 335 246 1012 86
Cuts at: 2946 2958 3304 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 396 380 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3752
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 627 1702 1883 2046 627
Size: 1075 181 163 2333
Fragments arranged by size:
2333 1075 181 163
BtsI GCAGTG_nn'
Cuts at: 995 995
Size: 3752
DraI TTT'AAA
Cuts at: 243 751 243
Size: 508 3244
DrdI GACnn_nn'nnGTC
Cuts at: 1458 2316 1458
Size: 858 2894
EaeI y'GGCC_r
Cuts at: 1337 1511 1902 1929 3274 1337
Size: 174 391 27 1345 1815
Fragments arranged by size:
1815 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1337 1337
Size: 3752
EarI CTCTTCn'nnn_
Cuts at: 479 1775 1985 479
Size: 1296 210 2246
Fragments arranged by size:
2246 1296 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 114 1960 2270 2416 3562 114
Size: 1846 310 146 1146 304
Fragments arranged by size:
1846 1146 310 304 146
EcoICRI GAG'CTC
Cuts at: 764 764
Size: 3752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 409 460 1577 2009 2756 3024 269
Size: 140 51 1117 432 747 268 997
Fragments arranged by size:
1117 997 747 432 268 140 51
EcoO109I rG'GnC_Cy
Cuts at: 891 891
Size: 3752
EcoRI G'AATT_C
Cuts at: 756 756
Size: 3752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 400 1771 400
Size: 1371 2381
FspI TGC'GCA
Cuts at: 1533 1533
Size: 3752
HaeII r_GCGC'y
Cuts at: 1186 1434 2456 3435 1186
Size: 248 1022 979 1503
Fragments arranged by size:
1503 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 670 702 1594 1624 1626 1656 670
Size: 32 892 30 2 30 2766
Fragments arranged by size:
2766 892 32 30 30 2
HincII GTy'rAC
Cuts at: 791 791
Size: 3752
KasI G'GCGC_C
Cuts at: 1182 1430 1182
Size: 248 3504
KpnI G_GTAC'C
Cuts at: 772 772
Size: 3752
MluI A'CGCG_T
Cuts at: 3185 3185
Size: 3752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2607 2423
Size: 184 3568
MscI TGG'CCA
Cuts at: 1513 1513
Size: 3752
MslI CAynn'nnrTG
Cuts at: 1741 1868 1741
Size: 127 3625
MspA1I CmG'CkG
Cuts at: 223 543 863 1177 1537 2550 2795 223
Size: 320 320 314 360 1013 245 1180
Fragments arranged by size:
1180 1013 360 320 320 314 245
NaeI GCC'GGC
Cuts at: 1933 1933
Size: 3752
NarI GG'CG_CC
Cuts at: 1183 1431 1183
Size: 248 3504
NcoI C'CATG_G
Cuts at: 107 107
Size: 3752
NgoMIV G'CCGG_C
Cuts at: 1931 1931
Size: 3752
NspI r_CATG'y
Cuts at: 805 1305 2212 805
Size: 500 907 2345
Fragments arranged by size:
2345 907 500
PciI A'CATG_T
Cuts at: 2208 2208
Size: 3752
PmeI GTTT'AAAC
Cuts at: 751 751
Size: 3752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 3720
PstI C_TGCA'G
Cuts at: 799 1484 799
Size: 685 3067
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3752
PvuII CAG'CTG
Cuts at: 223 1177 1537 223
Size: 954 360 2438
Fragments arranged by size:
2438 954 360
RsrII CG'GwC_CG
Cuts at: 1947 1947
Size: 3752
SacI G_AGCT'C
Cuts at: 766 766
Size: 3752
SalI G'TCGA_C
Cuts at: 789 789
Size: 3752
SapI GCTCTTCn'nnn_
Cuts at: 1775 1985 1775
Size: 210 3542
SbfI CC_TGCA'GG
Cuts at: 799 799
Size: 3752
ScaI AGT'ACT
Cuts at: 3269 3269
Size: 3752
SfcI C'TryA_G
Cuts at: 33 795 1480 2473 2664 33
Size: 762 685 993 191 1121
Fragments arranged by size:
1121 993 762 685 191
SfoI GGC'GCC
Cuts at: 1184 1432 1184
Size: 248 3504
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
SmaI CCC'GGG
Cuts at: 774 774
Size: 3752
SmlI C'TyrA_G
Cuts at: 595 907 1822 2314 2576 595
Size: 312 915 492 262 1771
Fragments arranged by size:
1771 915 492 312 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3752
SphI G_CATG'C
Cuts at: 805 1305 805
Size: 500 3252
SspI AAT'ATT
Cuts at: 347 347
Size: 3752
StyI C'CwwG_G
Cuts at: 107 886 1240 107
Size: 779 354 2619
Fragments arranged by size:
2619 779 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1335 1468 1927 2136 1335
Size: 133 459 209 2951
Fragments arranged by size:
2951 459 209 133
TatI w'GTAC_w
Cuts at: 355 3267 355
Size: 2912 840
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 3720
Tth111I GACn'n_nGTC
Cuts at: 197 1549 197
Size: 1352 2400
XbaI T'CTAG_A
Cuts at: 783 783
Size: 3752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1247 1247
Size: 3752
XmaI C'CCGG_G
Cuts at: 772 772
Size: 3752
XmnI GAAnn'nnTTC
Cuts at: 383 404 383
Size: 21 3731
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI