Restriction map for
pF1KB7910
(Circular) (Six-base) MAPSORT of: pF1KB7910.seq Check: 1170 from: 1 to: 4103
pF1KB7910 4103 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 830 830
Size: 4103
AccI GT'mk_AC
Cuts at: 1141 1141
Size: 4103
Acc65I G'GTAC_C
Cuts at: 1119 1119
Size: 4103
AclI AA'CG_TT
Cuts at: 3943 3943
Size: 4103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 945 1002 1928 2360 3107 3375 546
Size: 399 57 926 432 747 268 1274
Fragments arranged by size:
1274 926 747 432 399 268 57
AfeI AGC'GCT
Cuts at: 799 799
Size: 4103
AflIII A'CryG_T
Cuts at: 1455 2559 3536 1455
Size: 1104 977 2022
Fragments arranged by size:
2022 1104 977
AgeI A'CCGG_T
Cuts at: 2491 2491
Size: 4103
AleI CACnn'nnGTG
Cuts at: 920 920
Size: 4103
AlwNI CAG_nnn'CTG
Cuts at: 270 1408 2975 270
Size: 1138 1567 1398
Fragments arranged by size:
1567 1398 1138
ApaI G_GGCC'C
Cuts at: 352 352
Size: 4103
ApaLI G'TGCA_C
Cuts at: 1032 2873 1032
Size: 1841 2262
ApoI r'AATT_y
Cuts at: 1107 3456 3712 1107
Size: 2349 256 1498
Fragments arranged by size:
2349 1498 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4103
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
AvaI C'yCGr_G
Cuts at: 1123 1123
Size: 4103
BamHI G'GATC_C
Cuts at: 1128 1128
Size: 4103
BanI G'GyrC_C
Cuts at: 207 339 1070 1119 1533 1781 1816 207
Size: 132 731 49 414 248 35 2494
Fragments arranged by size:
2494 731 414 248 132 49 35
BanII G_rGCy'C
Cuts at: 352 551 641 973 1117 2147 352
Size: 199 90 332 144 1030 2308
Fragments arranged by size:
2308 1030 332 199 144 90
BbeI G_GCGC'C
Cuts at: 1537 1785 1537
Size: 248 3855
BbsI GAAGACnn'nnnn_
Cuts at: 612 612
Size: 4103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 690 724 90
Size: 34 566 34 3469
Fragments arranged by size:
3469 566 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2001 2762 4103 2001
Size: 761 1341 2001
Fragments arranged by size:
2001 1341 761
BclI T'GATC_A
Cuts at: 1623 1623
Size: 4103
BglI GCCn_nnn'nGGC
Cuts at: 331 521 331
Size: 190 3913
BglII A'GATC_T
Cuts at: 1618 3655 1618
Size: 2037 2066
BlpI GC'TnA_GC
Cuts at: 1215 1215
Size: 4103
Bme1580I G_kGCm'C
Cuts at: 212 352 539 1036 1728 1821 2877 212
Size: 140 187 497 692 93 1056 1438
Fragments arranged by size:
1438 1056 692 497 187 140 93
BmgBI CAC'GTC
Cuts at: 744 744
Size: 4103
BmrI ACTGGGnnnn_n'
Cuts at: 1315 1396 1731 3875 1315
Size: 81 335 2144 1543
Fragments arranged by size:
2144 1543 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 520 748 325
Size: 195 228 3680
Fragments arranged by size:
3680 228 195
Bpu10I CC'TnA_GC
Cuts at: 244 244
Size: 4103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1279 2158 2650 2948 1078
Size: 201 879 492 298 2233
Fragments arranged by size:
2233 879 492 298 201
BsaI GGTCTCn'nnnn_
Cuts at: 209 3807 209
Size: 3598 505
BsaAI yAC'GTr
Cuts at: 1456 1456
Size: 4103
BsaBI GATnn'nnATC
Cuts at: 6 1617 3654 6
Size: 1611 2037 455
Fragments arranged by size:
2037 1611 455
BsaHI Gr'CG_yC
Cuts at: 1534 1782 2484 3980 1534
Size: 248 702 1496 1657
Fragments arranged by size:
1657 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1292 1813 2491 2765 2912 3323 1292
Size: 521 678 274 147 411 2072
Fragments arranged by size:
2072 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 733 763 733
Size: 30 4073
BseYI C'CCAG_C
Cuts at: 538 1528 2286 2863 3359 538
Size: 990 758 577 496 1282
Fragments arranged by size:
1282 990 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 187
Size: 4103
BsiEI CG_ry'CG
Cuts at: 104 1645 1691 2899 104
Size: 1541 46 1208 1308
Fragments arranged by size:
1541 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 506 973 1036 1117 1895 2085 2877 506
Size: 467 63 81 778 190 792 1732
Fragments arranged by size:
1732 792 778 467 190 81 63
Bsp1286I G_dGCh'C
Cuts at: 212 352 506 539 551 641 973 1036 1117
Size: 140 154 33 12 90 332 63 81
Cuts at: 1117 1728 1821 1895 2085 2147 2877 212
Size: 611 93 74 190 62 730 1438
Fragments arranged by size:
1438 730 611 332 190 154 140 93
90 81 74 63 62 33 12
BspEI T'CCGG_A
Cuts at: 3323 3323
Size: 4103
BspHI T'CATG_A
Cuts at: 3279 3279
Size: 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 830 1070 1153 1367 1669 2050 830
Size: 240 83 214 302 381 2883
Fragments arranged by size:
2883 381 302 240 214 83
BsrBI CCG'CTC
Cuts at: 50 183 2395 2449 3933 4098 50
Size: 133 2212 54 1484 165 55
Fragments arranged by size:
2212 1484 165 133 55 54
BsrDI GCAATG_nn'
Cuts at: 2015 2015
Size: 4103
BsrFI r'CCGG_y
Cuts at: 464 2101 2282 2491 464
Size: 1637 181 209 2076
Fragments arranged by size:
2076 1637 209 181
BssSI C'ACGA_G
Cuts at: 2374 2732 2374
Size: 358 3745
BstAPI GCAn_nnn'nTGC
Cuts at: 1480 1480
Size: 4103
BstBI TT'CG_AA
Cuts at: 2464 2464
Size: 4103
BstYI r'GATC_y
Cuts at: 1 1128 1595 1618 1953 2199 3211 3297 3309
Size: 1127 467 23 335 246 1012 86 12
Cuts at: 3309 3655 1
Size: 346 449
Fragments arranged by size:
1127 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2053 2234 2397 2053
Size: 181 163 3759
Fragments arranged by size:
3759 181 163
BtsI GCAGTG_nn'
Cuts at: 1346 1346
Size: 4103
DraI TTT'AAA
Cuts at: 1102 1102
Size: 4103
DraIII CAC_nnn'GTG
Cuts at: 603 720 603
Size: 117 3986
DrdI GACnn_nn'nnGTC
Cuts at: 1809 2667 1809
Size: 858 3245
EaeI y'GGCC_r
Cuts at: 323 440 453 711 1688 1862 2253 2280 3625
Size: 117 13 258 977 174 391 27 1345
Cuts at: 3625 323
Size: 801
Fragments arranged by size:
1345 977 801 391 258 174 117 27
13
EagI C'GGCC_G
Cuts at: 1688 1688
Size: 4103
EarI CTCTTCn'nnn_
Cuts at: 124 2126 2336 124
Size: 2002 210 1891
Fragments arranged by size:
2002 1891 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2311 2621 2767 3913 2311
Size: 310 146 1146 2501
Fragments arranged by size:
2501 1146 310 146
EcoICRI GAG'CTC
Cuts at: 971 1115 971
Size: 144 3959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 520 546 748 945 1002 1928 2360 3107
Size: 195 26 202 197 57 926 432 747
Cuts at: 3107 3375 325
Size: 268 1053
Fragments arranged by size:
1053 926 747 432 268 202 197 195
57 26
EcoO109I rG'GnC_Cy
Cuts at: 348 349 471 671 1242 348
Size: 1 122 200 571 3209
Fragments arranged by size:
3209 571 200 122 1
EcoRI G'AATT_C
Cuts at: 1107 1107
Size: 4103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 438 2122 438
Size: 1684 2419
FspI TGC'GCA
Cuts at: 1884 1884
Size: 4103
HaeII r_GCGC'y
Cuts at: 801 1537 1785 2807 3786 801
Size: 736 248 1022 979 1118
Fragments arranged by size:
1118 1022 979 736 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 367 399 1945 1975 1977 2007 367
Size: 32 1546 30 2 30 2463
Fragments arranged by size:
2463 1546 32 30 30 2
HincII GTy'rAC
Cuts at: 1142 1142
Size: 4103
KasI G'GCGC_C
Cuts at: 1533 1781 1533
Size: 248 3855
KpnI G_GTAC'C
Cuts at: 1123 1123
Size: 4103
MluI A'CGCG_T
Cuts at: 3536 3536
Size: 4103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 419 914 998 2774 2958 419
Size: 495 84 1776 184 1564
Fragments arranged by size:
1776 1564 495 184 84
MscI TGG'CCA
Cuts at: 325 442 455 1864 325
Size: 117 13 1409 2564
Fragments arranged by size:
2564 1409 117 13
MslI CAynn'nnrTG
Cuts at: 920 2092 2219 920
Size: 1172 127 2804
Fragments arranged by size:
2804 1172 127
MspA1I CmG'CkG
Cuts at: 1022 1214 1528 1888 2901 3146 1022
Size: 192 314 360 1013 245 1979
Fragments arranged by size:
1979 1013 360 314 245 192
NaeI GCC'GGC
Cuts at: 2284 2284
Size: 4103
NarI GG'CG_CC
Cuts at: 1534 1782 1534
Size: 248 3855
NgoMIV G'CCGG_C
Cuts at: 2282 2282
Size: 4103
NspI r_CATG'y
Cuts at: 652 780 1156 1656 2563 652
Size: 128 376 500 907 2192
Fragments arranged by size:
2192 907 500 376 128
PciI A'CATG_T
Cuts at: 2559 2559
Size: 4103
PflMI CCAn_nnn'nTGG
Cuts at: 270 321 270
Size: 51 4052
PfoI T'CCnGG_A
Cuts at: 497 497
Size: 4103
PmeI GTTT'AAAC
Cuts at: 1102 1102
Size: 4103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 4071
PpuMI rG'GwC_Cy
Cuts at: 671 671
Size: 4103
PspOMI G'GGCC_C
Cuts at: 348 348
Size: 4103
PstI C_TGCA'G
Cuts at: 1150 1835 1150
Size: 685 3418
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4103
PvuII CAG'CTG
Cuts at: 1022 1528 1888 1022
Size: 506 360 3237
Fragments arranged by size:
3237 506 360
RsrII CG'GwC_CG
Cuts at: 2298 2298
Size: 4103
SacI G_AGCT'C
Cuts at: 973 1117 973
Size: 144 3959
SalI G'TCGA_C
Cuts at: 1140 1140
Size: 4103
SanDI GG'GwC_CC
Cuts at: 671 671
Size: 4103
SapI GCTCTTCn'nnn_
Cuts at: 124 2126 2336 124
Size: 2002 210 1891
Fragments arranged by size:
2002 1891 210
SbfI CC_TGCA'GG
Cuts at: 1150 1150
Size: 4103
ScaI AGT'ACT
Cuts at: 3620 3620
Size: 4103
SfcI C'TryA_G
Cuts at: 33 1146 1831 2824 3015 33
Size: 1113 685 993 191 1121
Fragments arranged by size:
1121 1113 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 331 331
Size: 4103
SfoI GGC'GCC
Cuts at: 1535 1783 1535
Size: 248 3855
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
SmaI CCC'GGG
Cuts at: 1125 1125
Size: 4103
SmlI C'TyrA_G
Cuts at: 1093 1258 2173 2665 2927 1093
Size: 165 915 492 262 2269
Fragments arranged by size:
2269 915 492 262 165
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4103
SphI G_CATG'C
Cuts at: 1156 1656 1156
Size: 500 3603
StuI AGG'CCT
Cuts at: 790 958 790
Size: 168 3935
StyI C'CwwG_G
Cuts at: 437 554 1237 1591 437
Size: 117 683 354 2949
Fragments arranged by size:
2949 683 354 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 832 1000 1686 1819 2278 2487 832
Size: 168 686 133 459 209 2448
Fragments arranged by size:
2448 686 459 209 168 133
TatI w'GTAC_w
Cuts at: 3618 3618
Size: 4103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 104 136 733 765 871 903 3620 3652 104
Size: 32 597 32 106 32 2717 32 555
Fragments arranged by size:
2717 597 555 106 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1900 1900
Size: 4103
XbaI T'CTAG_A
Cuts at: 1134 1134
Size: 4103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1598 1598
Size: 4103
XmaI C'CCGG_G
Cuts at: 1123 1123
Size: 4103
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BbvCI BfrBI BmtI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SacII SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI