Restriction map for pF1KF0165
 (Linear) (Six-base) MAPSORT of: FLJ00165.seq  Check: 5852  from: 1  to: 810



FLJ00165.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:13  ..

AccI GT'mk_AC

Cuts at:      0     656     810 
   Size:        656     154
 
Acc65I G'GTAC_C

Cuts at:      0     458     810 
   Size:        458     352
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     629     810 
   Size:        171     458     181

  Fragments arranged by size: 

                 458     181     171
 
AleI CACnn'nnGTG

Cuts at:      0     352     810 
   Size:        352     458
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     562     810 
   Size:        562     248
 
AlwNI CAG_nnn'CTG

Cuts at:      0     652     810 
   Size:        652     158
 
ApaI G_GGCC'C

Cuts at:      0      30     810 
   Size:         30     780
 
AvaI C'yCGr_G

Cuts at:      0     100     810 
   Size:        100     710
 
BanI G'GyrC_C

Cuts at:      0     379     430     458     810 
   Size:        379      51      28     352

  Fragments arranged by size: 

                 379     352      51      28
 
BanII G_rGCy'C

Cuts at:      0      30      59     277     470     742     810 
   Size:         30      29     218     193     272      68

  Fragments arranged by size: 

                 272     218     193      68      30      29
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     673     707     810 
   Size:        673      34     103

  Fragments arranged by size: 

                 673     103      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     166     810 
   Size:        166     644
 
BglII A'GATC_T

Cuts at:      0     255     616     810 
   Size:        255     361     194

  Fragments arranged by size: 

                 361     255     194
 
Bme1580I G_kGCm'C

Cuts at:      0      30     313     541     607     810 
   Size:         30     283     228      66     203

  Fragments arranged by size: 

                 283     228     203      66      30
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     316     810 
   Size:        316     494
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     810 
   Size:        244     566
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     673     750     810 
   Size:        673      77      60

  Fragments arranged by size: 

                 673      77      60
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     473     503     810 
   Size:        473      30     307

  Fragments arranged by size: 

                 473     307      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67     285     810 
   Size:         67     218     525

  Fragments arranged by size: 

                 525     218      67
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     443     810 
   Size:        443     367
 
Bsp1286I G_dGCh'C

Cuts at:      0      30      59     277     313     470     541     607     742 
   Size:         30      29     218      36     157      71      66     135

Cuts at:    742     810 
   Size:         68

  Fragments arranged by size: 

                 218     157     135      71      68      66      36      30
                  29
 
BspHI T'CATG_A

Cuts at:      0     249     810 
   Size:        249     561
 
BsrFI r'CCGG_y

Cuts at:      0     142     508     810 
   Size:        142     366     302

  Fragments arranged by size: 

                 366     302     142
 
BstBI TT'CG_AA

Cuts at:      0     770     810 
   Size:        770      40
 
BstEII G'GTnAC_C

Cuts at:      0     345     810 
   Size:        345     465
 
BstYI r'GATC_y

Cuts at:      0     255     609     616     810 
   Size:        255     354       7     194

  Fragments arranged by size: 

                 354     255     194       7
 
BtgI C'CryG_G

Cuts at:      0     413     810 
   Size:        413     397
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     343     810 
   Size:        343     467
 
EaeI y'GGCC_r

Cuts at:      0     167     371     403     810 
   Size:        167     204      32     407

  Fragments arranged by size: 

                 407     204     167      32
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     244     629     810 
   Size:        171      73     385     181

  Fragments arranged by size: 

                 385     181     171      73
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     748     810 
   Size:        748      62
 
EcoO109I rG'GnC_Cy

Cuts at:      0      27     104     588     810 
   Size:         27      77     484     222

  Fragments arranged by size: 

                 484     222      77      27
 
HaeII r_GCGC'y

Cuts at:      0     675     810 
   Size:        675     135
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     189     221     561     593     601     633     703     735 
   Size:        189      32     340      32       8      32      70      32

Cuts at:    735     810 
   Size:         75

  Fragments arranged by size: 

                 340     189      75      70      32      32      32      32
                   8
 
KpnI G_GTAC'C

Cuts at:      0     462     810 
   Size:        462     348
 
MscI TGG'CCA

Cuts at:      0     169     405     810 
   Size:        169     236     405

  Fragments arranged by size: 

                 405     236     169
 
MslI CAynn'nnrTG

Cuts at:      0     352     810 
   Size:        352     458
 
MspA1I CmG'CkG

Cuts at:      0     191     428     810 
   Size:        191     237     382

  Fragments arranged by size: 

                 382     237     191
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     333     810 
   Size:        333     477
 
PpuMI rG'GwC_Cy

Cuts at:      0     104     588     810 
   Size:        104     484     222

  Fragments arranged by size: 

                 484     222     104
 
PshAI GACnn'nnGTC

Cuts at:      0     685     810 
   Size:        685     125
 
PspOMI G'GGCC_C

Cuts at:      0      26     810 
   Size:         26     784
 
PstI C_TGCA'G

Cuts at:      0     196     671     810 
   Size:        196     475     139

  Fragments arranged by size: 

                 475     196     139
 
PvuII CAG'CTG

Cuts at:      0     191     810 
   Size:        191     619
 
SanDI GG'GwC_CC

Cuts at:      0     104     810 
   Size:        104     706
 
SexAI A'CCwGG_T

Cuts at:      0     791     810 
   Size:        791      19
 
SfcI C'TryA_G

Cuts at:      0     192     667     810 
   Size:        192     475     143

  Fragments arranged by size: 

                 475     192     143
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     166     810 
   Size:        166     644
 
SmaI CCC'GGG

Cuts at:      0     102     810 
   Size:        102     708
 
StuI AGG'CCT

Cuts at:      0      38     810 
   Size:         38     772
 
StyI C'CwwG_G

Cuts at:      0      81     810 
   Size:         81     729
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     697     738     810 
   Size:        697      41      72

  Fragments arranged by size: 

                 697      72      41
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     116     148     473     505     810 
   Size:        116      32     325      32     305

  Fragments arranged by size: 

                 325     305     116      32      32
 
XmaI C'CCGG_G

Cuts at:      0     100     810 
   Size:        100     710
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AleI     AlfI    AlwNI     ApaI     AvaI
     BanI    BanII     BcgI     BglI    BglII Bme1580I     BmrI     BpmI
     BsaI    BsaXI    BseRI    BsmBI Bsp1286I    BspHI    BsrFI    BstBI
   BstEII    BstYI     BtgI     DrdI     EaeI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I     KpnI     MscI     MslI   MspA1I    PflMI    PpuMI
    PshAI   PspOMI     PstI    PvuII    SanDI    SexAI     SfcI     SfiI
     SmaI     StuI     StyI    TaqII     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BlpI
    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     MfeI     MluI
     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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