Restriction map for
pF1KF0162
(Circular) (Six-base) MAPSORT of: pF1KF0162.seq Check: 2182 from: 1 to: 5228
pF1KF0162 5228 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1370 2266 1370
Size: 896 4332
Acc65I G'GTAC_C
Cuts at: 2244 2244
Size: 5228
AclI AA'CG_TT
Cuts at: 5068 5068
Size: 5228
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 682 1966 3053 3485 3838 4500 128
Size: 554 1284 1087 432 353 662 856
Fragments arranged by size:
1284 1087 856 662 554 432 353
AflIII A'CryG_T
Cuts at: 1214 1254 2580 4380 4661 1214
Size: 40 1326 1800 281 1781
Fragments arranged by size:
1800 1781 1326 281 40
AhdI GACnn_n'nnGTC
Cuts at: 1857 1857
Size: 5228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 560 560
Size: 5228
AlwNI CAG_nnn'CTG
Cuts at: 2533 3971 2533
Size: 1438 3790
ApaI G_GGCC'C
Cuts at: 1508 1508
Size: 5228
ApaLI G'TGCA_C
Cuts at: 4066 4066
Size: 5228
ApoI r'AATT_y
Cuts at: 2232 4581 4837 2232
Size: 2349 256 2623
Fragments arranged by size:
2623 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5228
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5228
AvaI C'yCGr_G
Cuts at: 2248 2248
Size: 5228
BamHI G'GATC_C
Cuts at: 2253 2253
Size: 5228
BanI G'GyrC_C
Cuts at: 1208 2074 2244 2658 2906 2941 1208
Size: 866 170 414 248 35 3495
Fragments arranged by size:
3495 866 414 248 170 35
BanII G_rGCy'C
Cuts at: 1018 1225 1508 1598 1757 1895 2242 3272 1018
Size: 207 283 90 159 138 347 1030 2974
Fragments arranged by size:
2974 1030 347 283 207 159 138 90
BbeI G_GCGC'C
Cuts at: 2662 2910 2662
Size: 248 4980
BbsI GAAGACnn'nnnn_
Cuts at: 1660 1660
Size: 5228
BbvCI CC'TCA_GC
Cuts at: 1804 1804
Size: 5228
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2098 2132 2098
Size: 34 5194
BciVI GTATCCnnnnn_n'
Cuts at: 1471 3126 4182 5228 1471
Size: 1655 1056 1046 1471
Fragments arranged by size:
1655 1471 1056 1046
BclI T'GATC_A
Cuts at: 2748 2748
Size: 5228
BglI GCCn_nnn'nGGC
Cuts at: 1957 1957
Size: 5228
BglII A'GATC_T
Cuts at: 1322 2084 2743 4780 1322
Size: 762 659 2037 1770
Fragments arranged by size:
2037 1770 762 659
BlpI GC'TnA_GC
Cuts at: 322 2340 322
Size: 2018 3210
Bme1580I G_kGCm'C
Cuts at: 953 1508 2853 2946 4070 953
Size: 555 1345 93 1124 2111
Fragments arranged by size:
2111 1345 1124 555 93
BmgBI CAC'GTC
Cuts at: 708 1255 2148 708
Size: 547 893 3788
Fragments arranged by size:
3788 893 547
BmrI ACTGGGnnnn_n'
Cuts at: 657 1231 1644 2440 2521 2856 5000 657
Size: 574 413 796 81 335 2144 885
Fragments arranged by size:
2144 885 796 574 413 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 258 589 3595 258
Size: 331 3006 1891
Fragments arranged by size:
3006 1891 331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 435 1002 1429 1753 1894 2077 271
Size: 164 567 427 324 141 183 3422
Fragments arranged by size:
3422 567 427 324 183 164 141
Bpu10I CC'TnA_GC
Cuts at: 1804 1804
Size: 5228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 2404 3283 3997 4295 566
Size: 1838 879 714 298 1499
Fragments arranged by size:
1838 1499 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 127 4932 127
Size: 4805 423
BsaAI yAC'GTr
Cuts at: 2581 2581
Size: 5228
BsaBI GATnn'nnATC
Cuts at: 6 2742 4779 6
Size: 2736 2037 455
Fragments arranged by size:
2736 2037 455
BsaHI Gr'CG_yC
Cuts at: 866 2659 2907 3609 5105 866
Size: 1793 248 702 1496 989
Fragments arranged by size:
1793 1496 989 702 248
BsaWI w'CCGG_w
Cuts at: 206 1316 2417 2938 3616 4027 4174 4448 206
Size: 1110 1101 521 678 411 147 274 986
Fragments arranged by size:
1110 1101 986 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 268 326 453 1162 1792 145
Size: 123 58 127 709 630 3581
Fragments arranged by size:
3581 709 630 127 123 58
BseYI C'CCAG_C
Cuts at: 784 1823 2653 3411 4076 4484 784
Size: 1039 830 758 665 408 1528
Fragments arranged by size:
1528 1039 830 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 520
Size: 5228
BsiEI CG_ry'CG
Cuts at: 104 2770 2816 4046 104
Size: 2666 46 1230 1286
Fragments arranged by size:
2666 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 764 782 1598 1895 2242 3020 3210 4070 764
Size: 18 816 297 347 778 190 860 1922
Fragments arranged by size:
1922 860 816 778 347 297 190 18
BsmBI CGTCTCn'nnnn_
Cuts at: 447 447
Size: 5228
Bsp1286I G_dGCh'C
Cuts at: 764 782 953 1018 1225 1508 1598 1757 1895
Size: 18 171 65 207 283 90 159 138
Cuts at: 1895 2242 2853 2946 3020 3210 3272 4070 764
Size: 347 611 93 74 190 62 798 1922
Fragments arranged by size:
1922 798 611 347 283 207 190 171
159 138 93 90 74 65 62 18
BspEI T'CCGG_A
Cuts at: 206 1316 206
Size: 1110 4118
BspHI T'CATG_A
Cuts at: 998 3660 998
Size: 2662 2566
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2278 2492 2794 3175 2278
Size: 214 302 381 4331
Fragments arranged by size:
4331 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1672 3520 3574 5058 5223 50
Size: 1622 1848 54 1484 165 55
Fragments arranged by size:
1848 1622 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2114 3140 2114
Size: 1026 4202
BsrFI r'CCGG_y
Cuts at: 1897 1932 3226 3407 1897
Size: 35 1294 181 3718
Fragments arranged by size:
3718 1294 181 35
BssSI C'ACGA_G
Cuts at: 3499 4207 3499
Size: 708 4520
BstAPI GCAn_nnn'nTGC
Cuts at: 1086 1442 2605 1086
Size: 356 1163 3709
Fragments arranged by size:
3709 1163 356
BstBI TT'CG_AA
Cuts at: 3589 3589
Size: 5228
BstXI CCAn_nnnn'nTGG
Cuts at: 489 916 1244 489
Size: 427 328 4473
Fragments arranged by size:
4473 427 328
BstYI r'GATC_y
Cuts at: 1 941 1322 2084 2253 2720 2743 3078 3324
Size: 940 381 762 169 467 23 335 246
Cuts at: 3324 3630 3642 3728 4780 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 940 762 467 449 381 335 306
246 169 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2069 2069
Size: 5228
BtgI C'CryG_G
Cuts at: 107 955 1031 1263 1707 1739 1775 107
Size: 848 76 232 444 32 36 3560
Fragments arranged by size:
3560 848 444 232 76 36 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3178 3359 3522 3178
Size: 181 163 4884
Fragments arranged by size:
4884 181 163
BtsI GCAGTG_nn'
Cuts at: 228 237 360 2471 228
Size: 9 123 2111 2985
Fragments arranged by size:
2985 2111 123 9
DraI TTT'AAA
Cuts at: 2227 2227
Size: 5228
DraIII CAC_nnn'GTG
Cuts at: 1239 1255 1239
Size: 16 5212
DrdI GACnn_nn'nnGTC
Cuts at: 631 2934 4278 631
Size: 2303 1344 1581
Fragments arranged by size:
2303 1581 1344
EaeI y'GGCC_r
Cuts at: 1565 1772 1922 1934 2813 2987 3378 3405 4750
Size: 207 150 12 879 174 391 27 1345
Cuts at: 4750 1565
Size: 2043
Fragments arranged by size:
2043 1345 879 391 207 174 150 27
12
EagI C'GGCC_G
Cuts at: 2813 2813
Size: 5228
EarI CTCTTCn'nnn_
Cuts at: 155 1354 1551 2129 3251 3461 155
Size: 1199 197 578 1122 210 1922
Fragments arranged by size:
1922 1199 1122 578 210 197
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3436 4178 4324 5038 3436
Size: 742 146 714 3626
Fragments arranged by size:
3626 742 714 146
EcoICRI GAG'CTC
Cuts at: 1596 1893 2240 1596
Size: 297 347 4584
Fragments arranged by size:
4584 347 297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 271 435 682 1002 1429 1753 1894 1966
Size: 143 164 247 320 427 324 141 72
Cuts at: 1966 2077 3053 3485 3838 4500 128
Size: 111 976 432 353 662 856
Fragments arranged by size:
976 856 662 432 427 353 324 320
247 164 143 141 111 72
EcoNI CCTnn'n_nnAGG
Cuts at: 450 538 1177 2032 2056 450
Size: 88 639 855 24 3622
Fragments arranged by size:
3622 855 639 88 24
EcoO109I rG'GnC_Cy
Cuts at: 1504 1505 2367 1504
Size: 1 862 4365
Fragments arranged by size:
4365 862 1
EcoRI G'AATT_C
Cuts at: 2232 2232
Size: 5228
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1089 3247 1089
Size: 2158 3070
FspI TGC'GCA
Cuts at: 3009 3009
Size: 5228
HaeII r_GCGC'y
Cuts at: 2662 2910 4140 4911 2662
Size: 248 1230 771 2979
Fragments arranged by size:
2979 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 124 156 1843 1875 2137 2169 3070 3100 3102
Size: 32 1687 32 262 32 901 30 2
Cuts at: 3102 3132 124
Size: 30 2220
Fragments arranged by size:
2220 1687 901 262 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2267 2267
Size: 5228
KasI G'GCGC_C
Cuts at: 2658 2906 2658
Size: 248 4980
KpnI G_GTAC'C
Cuts at: 2248 2248
Size: 5228
MluI A'CGCG_T
Cuts at: 4661 4661
Size: 5228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 491 2072 3987 4171 491
Size: 1581 1915 184 1548
Fragments arranged by size:
1915 1581 1548 184
MscI TGG'CCA
Cuts at: 1774 2989 1774
Size: 1215 4013
MslI CAynn'nnrTG
Cuts at: 1048 1129 1135 1273 1444 1768 2119 3217 3344
Size: 81 6 138 171 324 351 1098 127
Cuts at: 3344 1048
Size: 2932
Fragments arranged by size:
2932 1098 351 324 171 138 127 81
6
MspA1I CmG'CkG
Cuts at: 312 370 597 695 1944 2339 2653 3013 3797
Size: 58 227 98 1249 395 314 360 784
Cuts at: 3797 4042 312
Size: 245 1498
Fragments arranged by size:
1498 1249 784 395 360 314 245 227
98 58
NaeI GCC'GGC
Cuts at: 3409 3409
Size: 5228
NarI GG'CG_CC
Cuts at: 2659 2907 2659
Size: 248 4980
NcoI C'CATG_G
Cuts at: 107 1707 107
Size: 1600 3628
NgoMIV G'CCGG_C
Cuts at: 3407 3407
Size: 5228
NspI r_CATG'y
Cuts at: 1089 1218 2281 2781 4384 1089
Size: 129 1063 500 1603 1933
Fragments arranged by size:
1933 1603 1063 500 129
PasI CC'CwG_GG
Cuts at: 328 328
Size: 5228
PciI A'CATG_T
Cuts at: 1214 4380 1214
Size: 3166 2062
PflMI CCAn_nnn'nTGG
Cuts at: 920 1239 1708 1848 920
Size: 319 469 140 4300
Fragments arranged by size:
4300 469 319 140
PfoI T'CCnGG_A
Cuts at: 451 1018 1469 451
Size: 567 451 4210
Fragments arranged by size:
4210 567 451
PmeI GTTT'AAAC
Cuts at: 2227 2227
Size: 5228
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 5196
PshAI GACnn'nnGTC
Cuts at: 2037 2037
Size: 5228
PsiI TTA'TAA
Cuts at: 215 215
Size: 5228
PspOMI G'GGCC_C
Cuts at: 1504 1504
Size: 5228
PstI C_TGCA'G
Cuts at: 439 2275 2960 439
Size: 1836 685 2707
Fragments arranged by size:
2707 1836 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5228
PvuII CAG'CTG
Cuts at: 370 597 695 2653 3013 370
Size: 227 98 1958 360 2585
Fragments arranged by size:
2585 1958 360 227 98
RsrII CG'GwC_CG
Cuts at: 3423 3423
Size: 5228
SacI G_AGCT'C
Cuts at: 1598 1895 2242 1598
Size: 297 347 4584
Fragments arranged by size:
4584 347 297
SalI G'TCGA_C
Cuts at: 2265 2265
Size: 5228
SapI GCTCTTCn'nnn_
Cuts at: 2129 3251 3461 2129
Size: 1122 210 3896
Fragments arranged by size:
3896 1122 210
SbfI CC_TGCA'GG
Cuts at: 2275 2275
Size: 5228
ScaI AGT'ACT
Cuts at: 4745 4745
Size: 5228
SfcI C'TryA_G
Cuts at: 33 435 2271 2956 3924 4115 33
Size: 402 1836 685 968 191 1146
Fragments arranged by size:
1836 1146 968 685 402 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1957 1957
Size: 5228
SfoI GGC'GCC
Cuts at: 2660 2908 2660
Size: 248 4980
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5228
SmaI CCC'GGG
Cuts at: 2250 2250
Size: 5228
SmlI C'TyrA_G
Cuts at: 581 2383 3298 4012 4274 581
Size: 1802 915 714 262 1535
Fragments arranged by size:
1802 1535 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5228
SphI G_CATG'C
Cuts at: 1089 2281 2781 1089
Size: 1192 500 3536
Fragments arranged by size:
3536 1192 500
StyI C'CwwG_G
Cuts at: 107 344 1707 2362 2716 107
Size: 237 1363 655 354 2619
Fragments arranged by size:
2619 1363 655 354 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1250 1836 2811 2944 3403 3612 1250
Size: 586 975 133 459 209 2866
Fragments arranged by size:
2866 975 586 459 209 133
TatI w'GTAC_w
Cuts at: 794 4743 794
Size: 3949 1279
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 947 979 4745 4777 947
Size: 32 3766 32 1398
Fragments arranged by size:
3766 1398 32 32
Tth111I GACn'n_nGTC
Cuts at: 1659 3025 1659
Size: 1366 3862
XbaI T'CTAG_A
Cuts at: 2142 2259 2142
Size: 117 5111
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1518 2723 1518
Size: 1205 4023
XmaI C'CCGG_G
Cuts at: 2248 2248
Size: 5228
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AloI AscI
AvrII BaeI BfrBI BmtI BsaXI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PpuMI PspXI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI