Restriction map for
pF1KF0162
(Linear) (Six-base) MAPSORT of: FLJ00162.seq Check: 5603 from: 1 to: 2115
FLJ00162.orf
With 182 enzymes: SgfI *
March 3, 2008 11:13 ..
AccI GT'mk_AC
Cuts at: 0 1262 2115
Size: 1262 853
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 574 1858 2115
Size: 20 554 1284 257
Fragments arranged by size:
1284 554 257 20
AflIII A'CryG_T
Cuts at: 0 1106 1146 2115
Size: 1106 40 969
Fragments arranged by size:
1106 969 40
AhdI GACnn_n'nnGTC
Cuts at: 0 1749 2115
Size: 1749 366
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 452 2115
Size: 452 1663
ApaI G_GGCC'C
Cuts at: 0 1400 2115
Size: 1400 715
BanI G'GyrC_C
Cuts at: 0 1100 1966 2115
Size: 1100 866 149
Fragments arranged by size:
1100 866 149
BanII G_rGCy'C
Cuts at: 0 910 1117 1400 1490 1649 1787 2115
Size: 910 207 283 90 159 138 328
Fragments arranged by size:
910 328 283 207 159 138 90
BbsI GAAGACnn'nnnn_
Cuts at: 0 1552 2115
Size: 1552 563
BbvCI CC'TCA_GC
Cuts at: 0 1696 2115
Size: 1696 419
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1990 2024 2115
Size: 1990 34 91
Fragments arranged by size:
1990 91 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1363 2115
Size: 1363 752
BglI GCCn_nnn'nGGC
Cuts at: 0 1849 2115
Size: 1849 266
BglII A'GATC_T
Cuts at: 0 1214 1976 2115
Size: 1214 762 139
Fragments arranged by size:
1214 762 139
BlpI GC'TnA_GC
Cuts at: 0 214 2115
Size: 214 1901
Bme1580I G_kGCm'C
Cuts at: 0 845 1400 2115
Size: 845 555 715
Fragments arranged by size:
845 715 555
BmgBI CAC'GTC
Cuts at: 0 600 1147 2040 2115
Size: 600 547 893 75
Fragments arranged by size:
893 600 547 75
BmrI ACTGGGnnnn_n'
Cuts at: 0 549 1123 1536 2115
Size: 549 574 413 579
Fragments arranged by size:
579 574 549 413
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 150 481 2115
Size: 150 331 1634
Fragments arranged by size:
1634 331 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 327 894 1321 1645 1786 1969 2115
Size: 163 164 567 427 324 141 183 146
Fragments arranged by size:
567 427 324 183 164 163 146 141
Bpu10I CC'TnA_GC
Cuts at: 0 1696 2115
Size: 1696 419
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 2115
Size: 458 1657
BsaI GGTCTCn'nnnn_
Cuts at: 0 19 2115
Size: 19 2096
BsaHI Gr'CG_yC
Cuts at: 0 758 2115
Size: 758 1357
BsaWI w'CCGG_w
Cuts at: 0 98 1208 2115
Size: 98 1110 907
Fragments arranged by size:
1110 907 98
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 160 218 345 1054 1684 2115
Size: 37 123 58 127 709 630 431
Fragments arranged by size:
709 630 431 127 123 58 37
BseYI C'CCAG_C
Cuts at: 0 676 1715 2115
Size: 676 1039 400
Fragments arranged by size:
1039 676 400
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 2115
Size: 412 1703
BsiHKAI G_wGCw'C
Cuts at: 0 656 674 1490 1787 2115
Size: 656 18 816 297 328
Fragments arranged by size:
816 656 328 297 18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 339 2115
Size: 339 1776
Bsp1286I G_dGCh'C
Cuts at: 0 656 674 845 910 1117 1400 1490 1649
Size: 656 18 171 65 207 283 90 159
Cuts at: 1649 1787 2115
Size: 138 328
Fragments arranged by size:
656 328 283 207 171 159 138 90
65 18
BspEI T'CCGG_A
Cuts at: 0 98 1208 2115
Size: 98 1110 907
Fragments arranged by size:
1110 907 98
BspHI T'CATG_A
Cuts at: 0 890 2115
Size: 890 1225
BsrBI CCG'CTC
Cuts at: 0 1564 2115
Size: 1564 551
BsrDI GCAATG_nn'
Cuts at: 0 2006 2115
Size: 2006 109
BsrFI r'CCGG_y
Cuts at: 0 1789 1824 2115
Size: 1789 35 291
Fragments arranged by size:
1789 291 35
BstAPI GCAn_nnn'nTGC
Cuts at: 0 978 1334 2115
Size: 978 356 781
Fragments arranged by size:
978 781 356
BstXI CCAn_nnnn'nTGG
Cuts at: 0 381 808 1136 2115
Size: 381 427 328 979
Fragments arranged by size:
979 427 381 328
BstYI r'GATC_y
Cuts at: 0 833 1214 1976 2115
Size: 833 381 762 139
Fragments arranged by size:
833 762 381 139
Bsu36I CC'TnA_GG
Cuts at: 0 1961 2115
Size: 1961 154
BtgI C'CryG_G
Cuts at: 0 847 923 1155 1599 1631 1667 2115
Size: 847 76 232 444 32 36 448
Fragments arranged by size:
847 448 444 232 76 36 32
BtsI GCAGTG_nn'
Cuts at: 0 120 129 252 2115
Size: 120 9 123 1863
Fragments arranged by size:
1863 123 120 9
DraIII CAC_nnn'GTG
Cuts at: 0 1131 1147 2115
Size: 1131 16 968
Fragments arranged by size:
1131 968 16
DrdI GACnn_nn'nnGTC
Cuts at: 0 523 2115
Size: 523 1592
EaeI y'GGCC_r
Cuts at: 0 1457 1664 1814 1826 2115
Size: 1457 207 150 12 289
Fragments arranged by size:
1457 289 207 150 12
EarI CTCTTCn'nnn_
Cuts at: 0 47 1246 1443 2021 2115
Size: 47 1199 197 578 94
Fragments arranged by size:
1199 578 197 94 47
EcoICRI GAG'CTC
Cuts at: 0 1488 1785 2115
Size: 1488 297 330
Fragments arranged by size:
1488 330 297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 163 327 574 894 1321 1645 1786
Size: 20 143 164 247 320 427 324 141
Cuts at: 1786 1858 1969 2115
Size: 72 111 146
Fragments arranged by size:
427 324 320 247 164 146 143 141
111 72 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 342 430 1069 1924 1948 2115
Size: 342 88 639 855 24 167
Fragments arranged by size:
855 639 342 167 88 24
EcoO109I rG'GnC_Cy
Cuts at: 0 1396 1397 2115
Size: 1396 1 718
Fragments arranged by size:
1396 718 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 981 2115
Size: 981 1134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 1735 1767 2029 2061 2115
Size: 16 32 1687 32 262 32 54
Fragments arranged by size:
1687 262 54 32 32 32 16
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 1964 2115
Size: 383 1581 151
Fragments arranged by size:
1581 383 151
MscI TGG'CCA
Cuts at: 0 1666 2115
Size: 1666 449
MslI CAynn'nnrTG
Cuts at: 0 940 1021 1027 1165 1336 1660 2011 2115
Size: 940 81 6 138 171 324 351 104
Fragments arranged by size:
940 351 324 171 138 104 81 6
MspA1I CmG'CkG
Cuts at: 0 204 262 489 587 1836 2115
Size: 204 58 227 98 1249 279
Fragments arranged by size:
1249 279 227 204 98 58
NcoI C'CATG_G
Cuts at: 0 1599 2115
Size: 1599 516
NspI r_CATG'y
Cuts at: 0 981 1110 2115
Size: 981 129 1005
Fragments arranged by size:
1005 981 129
PasI CC'CwG_GG
Cuts at: 0 220 2115
Size: 220 1895
PciI A'CATG_T
Cuts at: 0 1106 2115
Size: 1106 1009
PflMI CCAn_nnn'nTGG
Cuts at: 0 812 1131 1600 1740 2115
Size: 812 319 469 140 375
Fragments arranged by size:
812 469 375 319 140
PfoI T'CCnGG_A
Cuts at: 0 343 910 1361 2115
Size: 343 567 451 754
Fragments arranged by size:
754 567 451 343
PshAI GACnn'nnGTC
Cuts at: 0 1929 2115
Size: 1929 186
PsiI TTA'TAA
Cuts at: 0 107 2115
Size: 107 2008
PspOMI G'GGCC_C
Cuts at: 0 1396 2115
Size: 1396 719
PstI C_TGCA'G
Cuts at: 0 331 2115
Size: 331 1784
PvuII CAG'CTG
Cuts at: 0 262 489 587 2115
Size: 262 227 98 1528
Fragments arranged by size:
1528 262 227 98
SacI G_AGCT'C
Cuts at: 0 1490 1787 2115
Size: 1490 297 328
Fragments arranged by size:
1490 328 297
SapI GCTCTTCn'nnn_
Cuts at: 0 2021 2115
Size: 2021 94
SfcI C'TryA_G
Cuts at: 0 327 2115
Size: 327 1788
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1849 2115
Size: 1849 266
SmlI C'TyrA_G
Cuts at: 0 473 2115
Size: 473 1642
SphI G_CATG'C
Cuts at: 0 981 2115
Size: 981 1134
StyI C'CwwG_G
Cuts at: 0 236 1599 2115
Size: 236 1363 516
Fragments arranged by size:
1363 516 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1142 1728 2115
Size: 1142 586 387
Fragments arranged by size:
1142 586 387
TatI w'GTAC_w
Cuts at: 0 686 2115
Size: 686 1429
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 839 871 2115
Size: 839 32 1244
Fragments arranged by size:
1244 839 32
Tth111I GACn'n_nGTC
Cuts at: 0 1551 2115
Size: 1551 564
XbaI T'CTAG_A
Cuts at: 0 2034 2115
Size: 2034 81
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1410 2115
Size: 1410 705
Enzymes that do cut:
AccI AcuI AflIII AhdI AlfI ApaI BanI BanII
BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I
BtgI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI Hin4I MmeI MscI MslI MspA1I
NcoI NspI PasI PciI PflMI PfoI PshAI PsiI
PspOMI PstI PvuII SacI SapI SfcI SfiI SmlI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BclI BfrBI BmtI BsaAI
BsaBI BsaXI BsiEI BsiWI BsmI BspMI BsrGI BssHII
BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
EagI EciI EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PpuMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
XhoI XmaI XmnI ZraI