Restriction map for pF1KF0068
##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AhdI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvrII       
BbrPI       BbsI        BclI        BfrI        BlnI        
BpuAI       BsaAI       BseAI       BsiWI       BsmBI       
BsmI        BspDI       BspEI       BspHI       BspLU11I    
BssHII      Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco52I      EcoRV       EcoT22I     FbaI        FseI        
KpnI        MfeI        MluI        MroI        MunI        
NaeI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
RcaI        RsrII       SalI        SbfI        ScaI        
SfiI        SfuI        SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AgeI         1950  ACCGGT               A^CCGGT
Alw44I       2692  GTGCAC               G^TGCAC
AocI         2013  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      2912  AGCGCT               AGC^GCT
ApaLI        2692  GTGCAC               G^TGCAC
Asp700I      2268  GAACTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI         508  GGATCC               G^GATCC
BbeI         2813  GGCGCC               GGCGC^C
BcgI          226  GCAGATGAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3387  GTATCC               GTATCC(6/5)
BlpI          798  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      798  GCTCAGC              GC^T[ACGT]AGC
BsaBI        2035  GATCGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1950  ACCGGT               [AT]^CCGG[AT]
Bsp1407I     3347  TGTACA               T^GTACA
BsrBI        2230  GAGCGG               CCGCTC(-3/-3)
BsrFI        1950  ACCGGT               [GA]^CCGG[CT]
BsrGI        3347  TGTACA               T^GTACA
BssSI        2414  CACGAG               CACGAG(-5/-1)
BstEII       1230  GGTGACC              G^GT[ACGT]ACC
BstPI        1230  GGTGACC              G^GT[ACGT]ACC
Bsu36I       2013  CCTGAGG              CC^T[ACGT]AGG
CelII         798  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1950  ACCGGT               [GA]^CCGG[CT]
DrdI         3187  GACCGCACCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2912  AGCGCT               AGC^GCT
Eco81I       2013  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1230  GGTGACC              G^GT[ACGT]ACC
EcoRI        1618  GAATTC               G^AATTC
HindIII      1924  AAGCTT               A^AGCTT
HpaI         2755  GTTAAC               GTT^AAC
KasI         2813  GGCGCC               G^GCGCC
KspI         2313  CCGCGG               CCGC^GG
MamI         2035  GATCGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI         2813  GGCGCC               GG^CGCC
NheI         1966  GCTAGC               G^CTAGC
NspI         1457  ACATGC               [GA]CATG^[CT]
PinAI        1950  ACCGGT               A^CCGGT
PvuI         2034  CGATCG               CGAT^CG
SacI         2659  GAGCTC               GAGCT^C
SacII        2313  CCGCGG               CCGC^GG
SfoI         2813  GGCGCC               GGC^GCC
SgrAI        1949  CACCGGTG             C[GA]^CCGG[CT]G
SspBI        3347  TGTACA               T^GTACA
TfiI         2040  GAATC                G^A[AT]TC
XbaI         2250  TCTAGA               T^CTAGA
XmnI         2268  GAACTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

ApaI         2646  GGGCCC               GGGCC^C
             2881  GGGCCC              
AviII        2804  TGCGCA               TGC^GCA
             3107  TGCGCA              
BglII        2978  AGATCT               A^GATCT
             3470  AGATCT              
BsiEI        2034  CGATCG               CG[GA][CT]^CG
             3186  CGACCG              
DraIII        347  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2943  CACTGAGTG           
FspI         2804  TGCGCA               TGC^GCA
             3107  TGCGCA              
HaeII        2813  GGCGCC               [GA]GCGC^[CT]
             2912  AGCGCT              
HgaI         2734  GACGC                GACGC(5/10)
             3235  GCGTC               
HphI          352  GGTGA                GGTGA(8/7)
             1230  GGTGA               
MaeII         125  ACGT                 A^CGT
             1699  ACGT                
MseI          128  TTAA                 T^TAA
             2756  TTAA                
MslI         3009  CACCTTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3129  CATGGGTGTG          
SexAI         836  ACCAGGT              A^CC[AT]GGT
             1496  ACCAGGT             
SmaI         1370  CCCGGG               CCC^GGG
             1749  CCCGGG              
Tru9I         128  TTAA                 T^TAA
             2756  TTAA                
Tsp45I        359  GTGAC                ^GT[GC]AC
             1231  GTGAC               
XcmI         1162  CCATGGCTGGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2588  CCATCCAGCGCATGG     
XmaCI        1370  CCCGGG               C^CCGGG
             1749  CCCGGG              
XmaI         1370  CCCGGG               C^CCGGG
             1749  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbvCI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrDI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PpuMI       PshAI       PstI        PvuII       
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        TseI        Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       
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