Restriction map for pF1KF0006
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflIII      AgeI        AhdI        AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AvrII       BamHI       BbrPI       
BbsI        BbvCI       BciVI       BfaI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BssHII      Bst1107I    
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoT22I     FseI        HincII      HindII      
HindIII     HpaI        KspI        MaeI        MamI        
MfeI        MluI        MroI        MunI        NcoI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
SexAI       SnaBI       SpeI        Sse8387I    SspI        
StuI        SwaI        TfiI        Tsp509I     Tth111I     
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

Acc65I       2051  GGTACC               G^GTACC
AflII        1702  CTTAAG               C^TTAAG
Alw44I       1341  GTGCAC               G^TGCAC
ApaLI        1341  GTGCAC               G^TGCAC
Asp718I      2051  GGTACC               G^GTACC
BclI          824  TGATCA               T^GATCA
BfrI         1702  CTTAAG               C^TTAAG
BglII          68  AGATCT               A^GATCT
BsaAI        1420  TACGTG               [CT]AC^GT[GA]
BsaI          454  GAGACC               GGTCTC(1/5)
BsmBI         294  CGTCTC               CGTCTC(1/5)
Bsp1407I      146  TGTACA               T^GTACA
BsrDI        1131  CATTGC               GCAATG(2/0)
BsrGI         146  TGTACA               T^GTACA
BstBI        1714  TTCGAA               TT^CGAA
DraIII        259  CACGATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          739  GACCAGCGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        1015  GATATC               GAT^ATC
EcoT14I      1454  CCAAGG               C^C[AT][AT]GG
FbaI          824  TGATCA               T^GATCA
KpnI         2051  GGTACC               GGTAC^C
MseI         1703  TTAA                 T^TAA
NdeI           54  CATATG               CA^TATG
NspV         1714  TTCGAA               TT^CGAA
ScaI         1628  AGTACT               AGT^ACT
SfiI         1698  GGCCCTTAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         1714  TTCGAA               TT^CGAA
SgrAI        1441  CACCGGCG             C[GA]^CCGG[CT]G
SspBI         146  TGTACA               T^GTACA
StyI         1454  CCAAGG               C^C[AT][AT]GG
Tru9I        1703  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AlwI          972  GATCC                GGATC(4/5)
              270  GGATC               
ApaI          386  GGGCCC               GGGCC^C
              566  GGGCCC              
AvaI          917  CCCGGG               C^[CT]CG[GA]G
             2079  CCCGGG              
BalI           74  TGGCCA               TGG^CCA
             1058  TGGCCA              
BbeI         1445  GGCGCC               GGCGC^C
             1750  GGCGCC              
BcgI         1776  CGAGGAGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2110  CGAGTCCTCTGC        
BglI         1571  GCCTGCGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1699  GCCCTTAAGGC         
BsoBI         917  CCCGGG               C^[CT]CG[GA]G
             2079  CCCGGG              
BssSI        1411  CACGAG               CACGAG(-5/-1)
             1675  CTCGTG              
BstYI          68  AGATCT               [GA]^GATC[CT]
              270  GGATCT              
DsaI         1094  CCGTGG               C^C[GA][CT]GG
             2039  CCGTGG              
KasI         1445  GGCGCC               G^GCGCC
             1750  GGCGCC              
MaeII        1421  ACGT                 A^CGT
             1919  ACGT                
MflI           68  AGATCT               [GA]^GATC[CT]
              270  GGATCT              
MluNI          74  TGGCCA               TGG^CCA
             1058  TGGCCA              
MscI           74  TGGCCA               TGG^CCA
             1058  TGGCCA              
NarI         1445  GGCGCC               GG^CGCC
             1750  GGCGCC              
PflMI         258  CCACGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1146  CCAGCTCCTGG         
PvuII         364  CAGCTG               CAG^CTG
             2229  CAGCTG              
SapI          964  GAAGAGC              GCTCTTC(1/4)
             1233  GCTCTTC             
SfoI         1445  GGCGCC               GGC^GCC
             1750  GGCGCC              
SmaI          917  CCCGGG               CCC^GGG
             2079  CCCGGG              
SphI          944  GCATGC               GCATG^C
             1938  GCATGC              
Van91I        258  CCACGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1146  CCAGCTCCTGG         
XcmI         1313  CCATCCTGCGCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1454  CCAAGGCCCTGCTGG     
XhoII          68  AGATCT               [GA]^GATC[CT]
              270  GGATCT              
XmaCI         917  CCCGGG               C^CCGGG
             2079  CCCGGG              
XmaI          917  CCCGGG               C^CCGGG
             2079  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AspHI       AvaII       AviII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoNI       
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PleI        PpuMI       PstI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     
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