(Circular) (Six-base) MAPSORT of: pF1KB9883.seq Check: 2843 from: 1 to: 4982 pF1KB9883 4982 bp With 182 enzymes: SgfI * June 20, 2008 10:32 .. AccI GT'mk_AC Cuts at: 2020 2020 Size: 4982 Acc65I G'GTAC_C Cuts at: 1998 1998 Size: 4982 AclI AA'CG_TT Cuts at: 390 4822 390 Size: 4432 550 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 102 856 988 2807 3239 3986 4254 102 Size: 754 132 1819 432 747 268 830 Fragments arranged by size: 1819 830 754 747 432 268 132 AfeI AGC'GCT Cuts at: 973 973 Size: 4982 AflIII A'CryG_T Cuts at: 2334 3438 4415 2334 Size: 1104 977 2901 Fragments arranged by size: 2901 1104 977 AgeI A'CCGG_T Cuts at: 3370 3370 Size: 4982 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 826 1355 826 Size: 529 4453 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1457 1489 1457 Size: 32 4950 AlwNI CAG_nnn'CTG Cuts at: 198 834 1503 2287 3854 198 Size: 636 669 784 1567 1326 Fragments arranged by size: 1567 1326 784 669 636 ApaLI G'TGCA_C Cuts at: 1669 3752 1669 Size: 2083 2899 ApoI r'AATT_y Cuts at: 1619 1986 4335 4591 1619 Size: 367 2349 256 2010 Fragments arranged by size: 2349 2010 367 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4982 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4982 AvaI C'yCGr_G Cuts at: 239 1128 2002 239 Size: 889 874 3219 Fragments arranged by size: 3219 889 874 BamHI G'GATC_C Cuts at: 2007 2007 Size: 4982 BanI G'GyrC_C Cuts at: 419 1238 1331 1998 2412 2660 2695 419 Size: 819 93 667 414 248 35 2706 Fragments arranged by size: 2706 819 667 414 248 93 35 BanII G_rGCy'C Cuts at: 820 1081 1174 1384 1445 1712 1996 3026 820 Size: 261 93 210 61 267 284 1030 2776 Fragments arranged by size: 2776 1030 284 267 261 210 93 61 BbeI G_GCGC'C Cuts at: 423 1242 2416 2664 423 Size: 819 1174 248 2741 Fragments arranged by size: 2741 1174 819 248 BbsI GAAGACnn'nnnn_ Cuts at: 677 677 Size: 4982 BbvCI CC'TCA_GC Cuts at: 1436 1528 1436 Size: 92 4890 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1208 1242 1208 Size: 34 4948 BciVI GTATCCnnnnn_n' Cuts at: 917 2880 3641 4982 917 Size: 1963 761 1341 917 Fragments arranged by size: 1963 1341 917 761 BclI T'GATC_A Cuts at: 1923 2502 1923 Size: 579 4403 BglI GCCn_nnn'nGGC Cuts at: 321 321 Size: 4982 BglII A'GATC_T Cuts at: 2497 4534 2497 Size: 2037 2945 BlpI GC'TnA_GC Cuts at: 2094 2094 Size: 4982 Bme1580I G_kGCm'C Cuts at: 695 1127 1673 2607 2700 3756 695 Size: 432 546 934 93 1056 1921 Fragments arranged by size: 1921 1056 934 546 432 93 BmrI ACTGGGnnnn_n' Cuts at: 2194 2275 2610 4754 2194 Size: 81 335 2144 2422 Fragments arranged by size: 2422 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1922 3349 1922 Size: 1427 3555 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 783 1021 1057 1210 1869 783 Size: 238 36 153 659 3896 Fragments arranged by size: 3896 659 238 153 36 Bpu10I CC'TnA_GC Cuts at: 361 950 1082 1378 1436 1528 361 Size: 589 132 296 58 92 3815 Fragments arranged by size: 3815 589 296 132 92 58 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 892 2158 3037 3529 3827 892 Size: 1266 879 492 298 2047 Fragments arranged by size: 2047 1266 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 608 4686 608 Size: 4078 904 BsaAI yAC'GTr Cuts at: 1668 2335 1668 Size: 667 4315 BsaBI GATnn'nnATC Cuts at: 6 2496 4533 6 Size: 2490 2037 455 Fragments arranged by size: 2490 2037 455 BsaHI Gr'CG_yC Cuts at: 420 1239 2413 2661 3363 4859 420 Size: 819 1174 248 702 1496 543 Fragments arranged by size: 1496 1174 819 702 543 248 BsaWI w'CCGG_w Cuts at: 214 2171 2692 3370 3644 3791 4202 214 Size: 1957 521 678 274 147 411 994 Fragments arranged by size: 1957 994 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1256 1286 1366 1396 1756 1786 1256 Size: 30 80 30 360 30 4452 Fragments arranged by size: 4452 360 80 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 575 976 1317 1453 1909 575 Size: 401 341 136 456 3648 Fragments arranged by size: 3648 456 401 341 136 BseYI C'CCAG_C Cuts at: 131 198 271 482 494 785 1943 2407 3165 Size: 67 73 211 12 291 1158 464 758 Cuts at: 3165 3742 4238 131 Size: 577 496 875 Fragments arranged by size: 1158 875 758 577 496 464 291 211 73 67 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 271 1714 1843 271 Size: 1443 129 3410 Fragments arranged by size: 3410 1443 129 BsiEI CG_ry'CG Cuts at: 104 502 2524 2570 3778 104 Size: 398 2022 46 1208 1308 Fragments arranged by size: 2022 1308 1208 398 46 BsiHKAI G_wGCw'C Cuts at: 238 334 1217 1384 1445 1673 1712 1996 2774 Size: 96 883 167 61 228 39 284 778 Cuts at: 2774 2964 3756 238 Size: 190 792 1464 Fragments arranged by size: 1464 883 792 778 284 228 190 167 96 61 39 BsmBI CGTCTCn'nnnn_ Cuts at: 910 910 Size: 4982 Bsp1286I G_dGCh'C Cuts at: 238 334 695 820 1081 1127 1174 1217 1384 Size: 96 361 125 261 46 47 43 167 Cuts at: 1384 1445 1673 1712 1996 2607 2700 2774 2964 Size: 61 228 39 284 611 93 74 190 Cuts at: 2964 3026 3756 238 Size: 62 730 1464 Fragments arranged by size: 1464 730 611 361 284 261 228 190 167 125 96 93 74 62 61 47 46 43 39 BspEI T'CCGG_A Cuts at: 214 4202 214 Size: 3988 994 BspHI T'CATG_A Cuts at: 4158 4158 Size: 4982 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2032 2246 2548 2929 2032 Size: 214 302 381 4085 Fragments arranged by size: 4085 381 302 214 BsrBI CCG'CTC Cuts at: 50 681 1005 1515 3274 3328 4812 4977 50 Size: 631 324 510 1759 54 1484 165 55 Fragments arranged by size: 1759 1484 631 510 324 165 55 54 BsrDI GCAATG_nn' Cuts at: 2894 2894 Size: 4982 BsrFI r'CCGG_y Cuts at: 404 1013 1139 2980 3161 3370 404 Size: 609 126 1841 181 209 2016 Fragments arranged by size: 2016 1841 609 209 181 126 BssHII G'CGCG_C Cuts at: 1198 1200 1578 1198 Size: 2 378 4602 Fragments arranged by size: 4602 378 2 BssSI C'ACGA_G Cuts at: 3253 3611 3253 Size: 358 4624 BstAPI GCAn_nnn'nTGC Cuts at: 1290 1821 2359 1290 Size: 531 538 3913 Fragments arranged by size: 3913 538 531 BstBI TT'CG_AA Cuts at: 3343 3343 Size: 4982 BstYI r'GATC_y Cuts at: 1 147 1646 2007 2474 2497 2832 3078 4090 Size: 146 1499 361 467 23 335 246 1012 Cuts at: 4090 4176 4188 4534 1 Size: 86 12 346 449 Fragments arranged by size: 1499 1012 467 449 361 346 335 246 146 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1447 1504 1720 1877 1447 Size: 57 216 157 4552 Fragments arranged by size: 4552 216 157 57 BtgI C'CryG_G Cuts at: 107 163 310 1343 107 Size: 56 147 1033 3746 Fragments arranged by size: 3746 1033 147 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 380 2932 3113 3276 380 Size: 2552 181 163 2086 Fragments arranged by size: 2552 2086 181 163 BtsI GCAGTG_nn' Cuts at: 340 632 1048 2225 340 Size: 292 416 1177 3097 Fragments arranged by size: 3097 1177 416 292 DraI TTT'AAA Cuts at: 1981 1981 Size: 4982 DraIII CAC_nnn'GTG Cuts at: 233 610 233 Size: 377 4605 DrdI GACnn_nn'nnGTC Cuts at: 647 2688 3546 647 Size: 2041 858 2083 Fragments arranged by size: 2083 2041 858 EaeI y'GGCC_r Cuts at: 499 2567 2741 3132 3159 4504 499 Size: 2068 174 391 27 1345 977 Fragments arranged by size: 2068 1345 977 391 174 27 EagI C'GGCC_G Cuts at: 499 2567 499 Size: 2068 2914 EarI CTCTTCn'nnn_ Cuts at: 733 832 845 1547 3005 3215 733 Size: 99 13 702 1458 210 2500 Fragments arranged by size: 2500 1458 702 210 99 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 188 1651 3190 3500 3646 4792 188 Size: 1463 1539 310 146 1146 378 Fragments arranged by size: 1539 1463 1146 378 310 146 EcoICRI GAG'CTC Cuts at: 1382 1443 1710 1994 1382 Size: 61 267 284 4370 Fragments arranged by size: 4370 284 267 61 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 102 783 856 988 1021 1057 1210 1869 2807 Size: 681 73 132 33 36 153 659 938 Cuts at: 2807 3239 3986 4254 102 Size: 432 747 268 830 Fragments arranged by size: 938 830 747 681 659 432 268 153 132 73 36 33 EcoNI CCTnn'n_nnAGG Cuts at: 1507 1507 Size: 4982 EcoO109I rG'GnC_Cy Cuts at: 380 831 1410 1500 2121 380 Size: 451 579 90 621 3241 Fragments arranged by size: 3241 621 579 451 90 EcoRI G'AATT_C Cuts at: 1619 1986 1619 Size: 367 4615 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3001 3001 Size: 4982 FspI TGC'GCA Cuts at: 1612 2763 1612 Size: 1151 3831 HaeII r_GCGC'y Cuts at: 423 975 1014 1242 1906 2416 2664 3686 4665 Size: 552 39 228 664 510 248 1022 979 Cuts at: 4665 423 Size: 740 Fragments arranged by size: 1022 979 740 664 552 510 248 228 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2824 2854 2856 2886 2824 Size: 30 2 30 4920 Fragments arranged by size: 4920 30 30 2 HincII GTy'rAC Cuts at: 2021 2021 Size: 4982 KasI G'GCGC_C Cuts at: 419 1238 2412 2660 419 Size: 819 1174 248 2741 Fragments arranged by size: 2741 1174 819 248 KpnI G_GTAC'C Cuts at: 2002 2002 Size: 4982 MluI A'CGCG_T Cuts at: 4415 4415 Size: 4982 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1674 3653 3837 1674 Size: 1979 184 2819 Fragments arranged by size: 2819 1979 184 MscI TGG'CCA Cuts at: 2743 2743 Size: 4982 MslI CAynn'nnrTG Cuts at: 1337 2971 3098 1337 Size: 1634 127 3221 Fragments arranged by size: 3221 1634 127 MspA1I CmG'CkG Cuts at: 165 195 312 486 498 1296 1455 1479 2093 Size: 30 117 174 12 798 159 24 614 Cuts at: 2093 2407 2767 3780 4025 165 Size: 314 360 1013 245 1122 Fragments arranged by size: 1122 1013 798 614 360 314 245 174 159 117 30 24 12 NaeI GCC'GGC Cuts at: 1015 3163 1015 Size: 2148 2834 NarI GG'CG_CC Cuts at: 420 1239 2413 2661 420 Size: 819 1174 248 2741 Fragments arranged by size: 2741 1174 819 248 NcoI C'CATG_G Cuts at: 107 107 Size: 4982 NgoMIV G'CCGG_C Cuts at: 1013 3161 1013 Size: 2148 2834 NotI GC'GGCC_GC Cuts at: 499 499 Size: 4982 NspI r_CATG'y Cuts at: 1342 2035 2535 3442 1342 Size: 693 500 907 2882 Fragments arranged by size: 2882 907 693 500 PasI CC'CwG_GG Cuts at: 531 531 Size: 4982 PciI A'CATG_T Cuts at: 3438 3438 Size: 4982 PflMI CCAn_nnn'nTGG Cuts at: 792 792 Size: 4982 PfoI T'CCnGG_A Cuts at: 394 1274 1364 1703 1856 1885 394 Size: 880 90 339 153 29 3491 Fragments arranged by size: 3491 880 339 153 90 29 PmeI GTTT'AAAC Cuts at: 1981 1981 Size: 4982 PmlI CAC'GTG Cuts at: 1668 1668 Size: 4982 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4121 4153 4121 Size: 32 4950 PpuMI rG'GwC_Cy Cuts at: 831 1410 831 Size: 579 4403 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 1957 1989 1957 Size: 32 4950 PstI C_TGCA'G Cuts at: 938 1295 1301 2029 2714 938 Size: 357 6 728 685 3206 Fragments arranged by size: 3206 728 685 357 6 PvuI CG_AT'CG Cuts at: 104 104 Size: 4982 PvuII CAG'CTG Cuts at: 486 1296 1455 2407 2767 486 Size: 810 159 952 360 2701 Fragments arranged by size: 2701 952 810 360 159 RsrII CG'GwC_CG Cuts at: 3177 3177 Size: 4982 SacI G_AGCT'C Cuts at: 1384 1445 1712 1996 1384 Size: 61 267 284 4370 Fragments arranged by size: 4370 284 267 61 SacII CC_GC'GG Cuts at: 166 313 166 Size: 147 4835 SalI G'TCGA_C Cuts at: 2019 2019 Size: 4982 SapI GCTCTTCn'nnn_ Cuts at: 832 1547 3005 3215 832 Size: 715 1458 210 2599 Fragments arranged by size: 2599 1458 715 210 SbfI CC_TGCA'GG Cuts at: 2029 2029 Size: 4982 ScaI AGT'ACT Cuts at: 4499 4499 Size: 4982 SexAI A'CCwGG_T Cuts at: 755 755 Size: 4982 SfcI C'TryA_G Cuts at: 33 934 1291 1297 2025 2710 3703 3894 33 Size: 901 357 6 728 685 993 191 1121 Fragments arranged by size: 1121 993 901 728 685 357 191 6 SfoI GGC'GCC Cuts at: 421 1240 2414 2662 421 Size: 819 1174 248 2741 Fragments arranged by size: 2741 1174 819 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4982 SmaI CCC'GGG Cuts at: 241 1130 2004 241 Size: 889 874 3219 Fragments arranged by size: 3219 889 874 SmlI C'TyrA_G Cuts at: 907 2137 3052 3544 3806 907 Size: 1230 915 492 262 2083 Fragments arranged by size: 2083 1230 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4982 SphI G_CATG'C Cuts at: 2035 2535 2035 Size: 500 4482 StuI AGG'CCT Cuts at: 955 1882 955 Size: 927 4055 StyI C'CwwG_G Cuts at: 107 791 1430 1836 2116 2470 107 Size: 684 639 406 280 354 2619 Fragments arranged by size: 2619 684 639 406 354 280 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 841 2565 2698 3157 3366 841 Size: 1724 133 459 209 2457 Fragments arranged by size: 2457 1724 459 209 133 TatI w'GTAC_w Cuts at: 4497 4497 Size: 4982 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 240 272 1126 1158 1756 1788 4499 4531 240 Size: 32 854 32 598 32 2711 32 691 Fragments arranged by size: 2711 854 691 598 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2779 2779 Size: 4982 XbaI T'CTAG_A Cuts at: 2013 2013 Size: 4982 XcmI CCAnnnn_n'nnnnTGG Cuts at: 195 2477 195 Size: 2282 2700 XmaI C'CCGG_G Cuts at: 239 1128 2002 239 Size: 889 874 3219 Fragments arranged by size: 3219 889 874 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AhdI AleI ApaI AscI AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI PacI PshAI PsiI PspOMI PspXI SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI