Restriction map for pF1KB9875
 (Linear) (Six-base) MAPSORT of: KIBB9875.seq  Check: 6478  from: 1  to: 1581



KIBB9875.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

Acc65I G'GTAC_C

Cuts at:      0    1290    1581 
   Size:       1290     291
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     422     583    1073    1163    1581 
   Size:        190     232     161     490      90     418

  Fragments arranged by size: 

                 490     418     232     190     161      90
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     769    1581 
   Size:        769     812
 
ApoI r'AATT_y

Cuts at:      0      66     562     808     893    1147    1192    1581 
   Size:         66     496     246      85     254      45     389

  Fragments arranged by size: 

                 496     389     254     246      85      66      45
 
AseI AT'TA_AT

Cuts at:      0     425    1581 
   Size:        425    1156
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1274    1307    1581 
   Size:       1274      33     274

  Fragments arranged by size: 

                1274     274      33
 
BanI G'GyrC_C

Cuts at:      0    1290    1581 
   Size:       1290     291
 
BanII G_rGCy'C

Cuts at:      0    1313    1461    1581 
   Size:       1313     148     120

  Fragments arranged by size: 

                1313     148     120
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1142    1581 
   Size:       1142     439
 
Bme1580I G_kGCm'C

Cuts at:      0    1355    1581 
   Size:       1355     226
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1365    1581 
   Size:       1365     216
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     759    1581 
   Size:        149     610     822

  Fragments arranged by size: 

                 822     610     149
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     901    1581 
   Size:        901     680
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     200    1581 
   Size:        200    1381
 
BseYI C'CCAG_C

Cuts at:      0     222     732    1581 
   Size:        222     510     849

  Fragments arranged by size: 

                 849     510     222
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     354    1181    1581 
   Size:        354     827     400

  Fragments arranged by size: 

                 827     400     354
 
BsiHKAI G_wGCw'C

Cuts at:      0    1313    1461    1581 
   Size:       1313     148     120

  Fragments arranged by size: 

                1313     148     120
 
Bsp1286I G_dGCh'C

Cuts at:      0    1313    1355    1461    1581 
   Size:       1313      42     106     120

  Fragments arranged by size: 

                1313     120     106      42
 
BsrDI GCAATG_nn'

Cuts at:      0    1440    1581 
   Size:       1440     141
 
BsrGI T'GTAC_A

Cuts at:      0     434     698    1581 
   Size:        434     264     883

  Fragments arranged by size: 

                 883     434     264
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     429    1581 
   Size:        429    1152
 
BstYI r'GATC_y

Cuts at:      0     115    1110    1377    1581 
   Size:        115     995     267     204

  Fragments arranged by size: 

                 995     267     204     115
 
BtgI C'CryG_G

Cuts at:      0     279    1581 
   Size:        279    1302
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1113    1148    1581 
   Size:       1113      35     433

  Fragments arranged by size: 

                1113     433      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     218    1064    1581 
   Size:        218     846     517

  Fragments arranged by size: 

                 846     517     218
 
EcoICRI GAG'CTC

Cuts at:      0    1311    1459    1581 
   Size:       1311     148     122

  Fragments arranged by size: 

                1311     148     122
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     190     422     583     759    1073    1163    1581 
   Size:        149      41     232     161     176     314      90     418

  Fragments arranged by size: 

                 418     314     232     176     161     149      90      41
            
 
EcoRV GAT'ATC

Cuts at:      0    1278    1581 
   Size:       1278     303
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1575    1581 
   Size:       1575       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     595     627    1581 
   Size:        595      32     954

  Fragments arranged by size: 

                 954     595      32
 
KpnI G_GTAC'C

Cuts at:      0    1294    1581 
   Size:       1294     287
 
MslI CAynn'nnrTG

Cuts at:      0     143     158     427    1346    1581 
   Size:        143      15     269     919     235

  Fragments arranged by size: 

                 919     269     235     143      15
 
MspA1I CmG'CkG

Cuts at:      0     736    1581 
   Size:        736     845
 
NcoI C'CATG_G

Cuts at:      0     279    1581 
   Size:        279    1302
 
NspI r_CATG'y

Cuts at:      0     486    1256    1444    1581 
   Size:        486     770     188     137

  Fragments arranged by size: 

                 770     486     188     137
 
PasI CC'CwG_GG

Cuts at:      0     723    1581 
   Size:        723     858
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1379    1411    1581 
   Size:       1379      32     170

  Fragments arranged by size: 

                1379     170      32
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     380     412    1509    1541    1581 
   Size:        380      32    1097      32      40

  Fragments arranged by size: 

                1097     380      40      32      32
 
PstI C_TGCA'G

Cuts at:      0      11    1581 
   Size:         11    1570
 
PvuII CAG'CTG

Cuts at:      0     736    1581 
   Size:        736     845
 
SacI G_AGCT'C

Cuts at:      0    1313    1461    1581 
   Size:       1313     148     120

  Fragments arranged by size: 

                1313     148     120
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1064    1581 
   Size:       1064     517
 
SfcI C'TryA_G

Cuts at:      0       7    1581 
   Size:          7    1574
 
StyI C'CwwG_G

Cuts at:      0     279    1581 
   Size:        279    1302
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     445     557    1581 
   Size:        445     112    1024

  Fragments arranged by size: 

                1024     445     112
 
TatI w'GTAC_w

Cuts at:      0     434     698    1581 
   Size:        434     264     883

  Fragments arranged by size: 

                 883     434     264
 

 Enzymes that do cut:

   Acc65I     AcuI     AlfI     ApoI     AseI     BaeI     BanI    BanII
     BbsI Bme1580I     BmrI     BpmI     BsaI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BsrDI    BsrGI    BstXI    BstYI     BtgI    CspCI
     EarI  EcoICRI  Eco57MI    EcoRV     FalI    Hin4I     KpnI     MslI
   MspA1I     NcoI     NspI     PasI     PpiI     PsrI     PstI    PvuII
     SacI     SapI     SfcI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI
 EcoO109I    EcoRI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com