Restriction map for pF1KB9866
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvrII       BamHI       BcgI        
BciVI       BclI        BfrI        BlnI        BlpI        
Bpu1102I    BsaBI       BseAI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DrdI        Eam1105I    
Eco47III    Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HindIII     HpaI        KpnI        MamI        MfeI        
MluI        MroI        MseI        MunI        NcoI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SalI        SapI        SbfI        
SexAI       SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tru9I       Tsp509I     TspRI       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AflIII        128  ACGTGT               A^C[GA][CT]GT
AlwI          751  GATCC                GGATC(4/5)
ApaI          866  GGGCCC               GGGCC^C
AspHI          46  GAGCAC               G[AT]GC[AT]^C
BalI         1298  TGGCCA               TGG^CCA
BanI         1110  GGCGCC               G^G[CT][GA]CC
BbeI         1110  GGCGCC               GGCGC^C
BbrPI         283  CACGTG               CAC^GTG
BbvCI        1096  GCTGAGG              CCTCAGC(-5/-2)
BfaI          898  CTAG                 C^TAG
BglII         662  AGATCT               A^GATCT
BsaAI         283  CACGTG               [CT]AC^GT[GA]
BsaI         1235  GAGACC               GGTCTC(1/5)
BsaWI        1287  ACCGGA               [AT]^CCGG[AT]
BsgI          289  CTGCAC               GTGCAG(16/14)
BsiHKAI        46  GAGCAC               G[AT]GC[AT]^C
BsmAI        1235  GAGAC                GTCTC(1/5)
BsrFI         493  GCCGGC               [GA]^CCGG[CT]
BssHII        913  GCGCGC               G^CGCGC
BssSI        1090  CACGAG               CACGAG(-5/-1)
BstAPI        291  GCACATCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         560  CCAACACGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         662  AGATCT               [GA]^GATC[CT]
Cfr10I        493  GCCGGC               [GA]^CCGG[CT]
DraIII        292  CACATCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I       365  CCAAGG               C^C[AT][AT]GG
HinfI          26  GACTC                G^A[ACGT]TC
HphI          155  TCACC                GGTGA(8/7)
KasI         1110  GGCGCC               G^GCGCC
MaeI          898  CTAG                 C^TAG
MflI          662  AGATCT               [GA]^GATC[CT]
MluNI        1298  TGGCCA               TGG^CCA
MscI         1298  TGGCCA               TGG^CCA
NaeI          493  GCCGGC               GCC^GGC
NarI         1110  GGCGCC               GG^CGCC
NgoMIV        493  GCCGGC               G^CCGGC
NheI          897  GCTAGC               G^CTAGC
NspI          653  ACATGC               [GA]CATG^[CT]
PleI           26  GACTC                GAGTC(4/5)
PmaCI         283  CACGTG               CAC^GTG
PmlI          283  CACGTG               CAC^GTG
PpuMI        1165  GGGACCC              [GA]G^G[AT]CC[CT]
PvuII         340  CAGCTG               CAG^CTG
ScaI          500  AGTACT               AGT^ACT
SfoI         1110  GGCGCC               GGC^GCC
SmaI          862  CCCGGG               CCC^GGG
StyI          365  CCAAGG               C^C[AT][AT]GG
XhoII         662  AGATCT               [GA]^GATC[CT]
XmaCI         862  CCCGGG               C^CCGGG
XmaI          862  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcyI          721  GACGCC               G[GA]^CG[CT]C
             1110  GGCGCC              
AfaI          501  GTAC                 GT^AC
              510  GTAC                
AvaI          862  CCCGGG               C^[CT]CG[GA]G
             1171  CCCGAG              
AvaII         440  GGACC                G^G[AT]CC
             1166  GGACC               
BanII         354  GAGCCC               G[GA]GC[CT]^C
              866  GGGCCC              
BsaHI         721  GACGCC               G[GA]^CG[CT]C
             1110  GGCGCC              
BsoBI         862  CCCGGG               C^[CT]CG[GA]G
             1171  CCCGAG              
BspMI         157  ACCTGC               ACCTGC(4/8)
              959  ACCTGC              
BstF5I        222  CATCC                GGATG(2/0)
             1125  GGATG               
EagI          778  CGGCCG               C^GGCCG
              995  CGGCCG              
EarI          307  GAAGAG               CTCTTC(1/4)
              345  GAAGAG              
EclXI         778  CGGCCG               C^GGCCG
              995  CGGCCG              
Eco52I        778  CGGCCG               C^GGCCG
              995  CGGCCG              
EcoNI         834  CCTGGGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              903  CCTGACCGAGG         
FokI          222  CATCC                GGATG(9/13)
             1125  GGATG               
Hin1I         721  GACGCC               G[GA]^CG[CT]C
             1110  GGCGCC              
Ksp632I       307  GAAGAG               CTCTTC(1/4)
              345  GAAGAG              
MaeIII        700  GTGAC                ^GT[ACGT]AC
             1192  GTGAC               
PshAI         441  GACCTACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1237  GACCCAGGTC          
PstI          238  CTGCAG               CTGCA^G
             1046  CTGCAG              
RsaI          501  GTAC                 GT^AC
              510  GTAC                
SfaNI        1209  GATGC                GCATC(5/9)
               98  GCATC               
SfcI          238  CTGCAG               C^T[GA][CT]AG
             1046  CTGCAG              
SfiI         1248  GGCCAGCAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1257  GGCCAGCAGGGCC       
SmlI          688  CTCAAG               C^T[CT][GA]AG
             1018  CTCAAG              
TaqI          476  TCGA                 T^CGA
              518  TCGA                
Tsp45I        700  GTGAC                ^GT[GC]AC
             1192  GTGAC               
TthHB8I       476  TCGA                 T^CGA
              518  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AccII       AciI        AluI        AviII       BbsI        
BbvI        BcnI        BglI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsiEI       BsiYI       
BslI        BsmFI       Bsp1286I    BsrBI       BsrI        
BssKI       BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HpaII       ItaI        KspI        
MaeII       MboI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       SacII       
Sau3AI      Sau96I      ScrFI       TseI        
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