Restriction map for pF1KB9866
 (Linear) (Six-base) MAPSORT of: KIBB9866.seq  Check: 199  from: 1  to: 1305



KIBB9866.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     166     968    1305 
   Size:        166     802     337

  Fragments arranged by size: 

                 802     337     166
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     544    1305 
   Size:        364     180     761

  Fragments arranged by size: 

                 761     364     180
 
AflIII A'CryG_T

Cuts at:      0     128    1305 
   Size:        128    1177
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     121     842    1305 
   Size:        121     721     463

  Fragments arranged by size: 

                 721     463     121
 
ApaI G_GGCC'C

Cuts at:      0     870    1305 
   Size:        870     435
 
AvaI C'yCGr_G

Cuts at:      0     862    1171    1305 
   Size:        862     309     134

  Fragments arranged by size: 

                 862     309     134
 
BanI G'GyrC_C

Cuts at:      0    1110    1305 
   Size:       1110     195
 
BanII G_rGCy'C

Cuts at:      0     358     870    1305 
   Size:        358     512     435

  Fragments arranged by size: 

                 512     435     358
 
BbeI G_GCGC'C

Cuts at:      0    1114    1305 
   Size:       1114     191
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      30     441     604    1305 
   Size:         30     411     163     701

  Fragments arranged by size: 

                 701     411     163      30
 
BbvCI CC'TCA_GC

Cuts at:      0    1097    1305 
   Size:       1097     208
 
BglI GCCn_nnn'nGGC

Cuts at:      0     713     730     991    1064    1255    1264    1305 
   Size:        713      17     261      73     191       9      41

  Fragments arranged by size: 

                 713     261     191      73      41      17       9
 
BglII A'GATC_T

Cuts at:      0     662    1305 
   Size:        662     643
 
Bme1580I G_kGCm'C

Cuts at:      0     870    1305 
   Size:        870     435
 
BmgBI CAC'GTC

Cuts at:      0     129    1305 
   Size:        129    1176
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     990    1305 
   Size:        990     315
 
BmtI G_CTAG'C

Cuts at:      0     901    1305 
   Size:        901     404
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30     567     679    1165    1305 
   Size:         30     537     112     486     140

  Fragments arranged by size: 

                 537     486     140     112      30
 
Bpu10I CC'TnA_GC

Cuts at:      0    1097    1305 
   Size:       1097     208
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     673    1003    1305 
   Size:        673     330     302

  Fragments arranged by size: 

                 673     330     302
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1229    1305 
   Size:       1229      76
 
BsaAI yAC'GTr

Cuts at:      0     285    1305 
   Size:        285    1020
 
BsaHI Gr'CG_yC

Cuts at:      0     722    1111    1305 
   Size:        722     389     194

  Fragments arranged by size: 

                 722     389     194
 
BsaWI w'CCGG_w

Cuts at:      0    1287    1305 
   Size:       1287      18
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     115     145    1305 
   Size:        115      30    1160

  Fragments arranged by size: 

                1160     115      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     343     346     349    1189    1192    1195    1305 
   Size:        343       3       3     840       3       3     110

  Fragments arranged by size: 

                 840     343     110       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     108     167     358     738     769    1054    1305 
   Size:        108      59     191     380      31     285     251

  Fragments arranged by size: 

                 380     285     251     191     108      59      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274    1305 
   Size:        274    1031
 
BsiEI CG_ry'CG

Cuts at:      0     405     546     781     998    1305 
   Size:        405     141     235     217     307

  Fragments arranged by size: 

                 405     307     235     217     141
 
BsiHKAI G_wGCw'C

Cuts at:      0      50    1305 
   Size:         50    1255
 
Bsp1286I G_dGCh'C

Cuts at:      0      50     358     870    1305 
   Size:         50     308     512     435

  Fragments arranged by size: 

                 512     435     308      50
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     166     968    1305 
   Size:        166     802     337

  Fragments arranged by size: 

                 802     337     166
 
BsrBI CCG'CTC

Cuts at:      0     531    1002    1133    1305 
   Size:        531     471     131     172

  Fragments arranged by size: 

                 531     471     172     131
 
BsrFI r'CCGG_y

Cuts at:      0     493    1305 
   Size:        493     812
 
BssHII G'CGCG_C

Cuts at:      0     913    1305 
   Size:        913     392
 
BssSI C'ACGA_G

Cuts at:      0    1090    1305 
   Size:       1090     215
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     297    1305 
   Size:        297    1008
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     567    1305 
   Size:        567     738
 
BstYI r'GATC_y

Cuts at:      0     662    1305 
   Size:        662     643
 
BtgI C'CryG_G

Cuts at:      0      53     116     405     590     851     881    1199    1305 
   Size:         53      63     289     185     261      30     318     106

  Fragments arranged by size: 

                 318     289     261     185     106      63      53      30
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     152     474    1305 
   Size:        152     322     831

  Fragments arranged by size: 

                 831     322     152
 
DraIII CAC_nnn'GTG

Cuts at:      0     297    1305 
   Size:        297    1008
 
EaeI y'GGCC_r

Cuts at:      0     422     495     778     948     995    1298    1305 
   Size:        422      73     283     170      47     303       7

  Fragments arranged by size: 

                 422     303     283     170      73      47       7
 
EagI C'GGCC_G

Cuts at:      0     778     995    1305 
   Size:        778     217     310

  Fragments arranged by size: 

                 778     310     217
 
EarI CTCTTCn'nnn_

Cuts at:      0     302     340    1305 
   Size:        302      38     965

  Fragments arranged by size: 

                 965     302      38
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     136     921    1305 
   Size:        136     785     384

  Fragments arranged by size: 

                 785     384     136
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30     364     544     567     679    1165    1305 
   Size:         30     334     180      23     112     486     140

  Fragments arranged by size: 

                 486     334     180     140     112      30      23
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     838     907    1305 
   Size:        838      69     398

  Fragments arranged by size: 

                 838     398      69
 
EcoO109I rG'GnC_Cy

Cuts at:      0     203     732     866     867    1166    1305 
   Size:        203     529     134       1     299     139

  Fragments arranged by size: 

                 529     299     203     139     134       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     621    1305 
   Size:        621     684
 
FspI TGC'GCA

Cuts at:      0     301     418     818    1305 
   Size:        301     117     400     487

  Fragments arranged by size: 

                 487     400     301     117
 
FspAI rTGC'GCAy

Cuts at:      0     301     418     818    1305 
   Size:        301     117     400     487

  Fragments arranged by size: 

                 487     400     301     117
 
HaeII r_GCGC'y

Cuts at:      0     238     433    1114    1157    1305 
   Size:        238     195     681      43     148

  Fragments arranged by size: 

                 681     238     195     148      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162     373     405     735     767    1305 
   Size:        130      32     211      32     330      32     538

  Fragments arranged by size: 

                 538     330     211     130      32      32      32
 
KasI G'GCGC_C

Cuts at:      0    1110    1305 
   Size:       1110     195
 
MscI TGG'CCA

Cuts at:      0    1300    1305 
   Size:       1300       5
 
MslI CAynn'nnrTG

Cuts at:      0     103     250     616    1305 
   Size:        103     147     366     689

  Fragments arranged by size: 

                 689     366     147     103
 
MspA1I CmG'CkG

Cuts at:      0     118     342     407     539     548     738     826     883 
   Size:        118     224      65     132       9     190      88      57

Cuts at:    883    1201    1274    1305 
   Size:        318      73      31

  Fragments arranged by size: 

                 318     224     190     132     118      88      73      65
                  57      31       9
 
NaeI GCC'GGC

Cuts at:      0     495    1305 
   Size:        495     810
 
NarI GG'CG_CC

Cuts at:      0    1111    1305 
   Size:       1111     194
 
NgoMIV G'CCGG_C

Cuts at:      0     493    1305 
   Size:        493     812
 
NheI G'CTAG_C

Cuts at:      0     897    1305 
   Size:        897     408
 
NspI r_CATG'y

Cuts at:      0     657    1305 
   Size:        657     648
 
PasI CC'CwG_GG

Cuts at:      0     834    1305 
   Size:        834     471
 
PmlI CAC'GTG

Cuts at:      0     285    1305 
   Size:        285    1020
 
PpuMI rG'GwC_Cy

Cuts at:      0    1166    1305 
   Size:       1166     139
 
PshAI GACnn'nnGTC

Cuts at:      0     445    1241    1305 
   Size:        445     796      64

  Fragments arranged by size: 

                 796     445      64
 
PspOMI G'GGCC_C

Cuts at:      0     866    1305 
   Size:        866     439
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     178     210    1305 
   Size:        178      32    1095

  Fragments arranged by size: 

                1095     178      32
 
PstI C_TGCA'G

Cuts at:      0     242    1050    1305 
   Size:        242     808     255

  Fragments arranged by size: 

                 808     255     242
 
PvuII CAG'CTG

Cuts at:      0     342    1305 
   Size:        342     963
 
SacII CC_GC'GG

Cuts at:      0     119     408     884    1202    1305 
   Size:        119     289     476     318     103

  Fragments arranged by size: 

                 476     318     289     119     103
 
SanDI GG'GwC_CC

Cuts at:      0    1166    1305 
   Size:       1166     139
 
ScaI AGT'ACT

Cuts at:      0     502    1305 
   Size:        502     803
 
SfcI C'TryA_G

Cuts at:      0     238    1046    1305 
   Size:        238     808     259

  Fragments arranged by size: 

                 808     259     238
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1255    1264    1305 
   Size:       1255       9      41

  Fragments arranged by size: 

                1255      41       9
 
SfoI GGC'GCC

Cuts at:      0    1112    1305 
   Size:       1112     193
 
SmaI CCC'GGG

Cuts at:      0     864    1305 
   Size:        864     441
 
SmlI C'TyrA_G

Cuts at:      0     688    1018    1305 
   Size:        688     330     287

  Fragments arranged by size: 

                 688     330     287
 
StyI C'CwwG_G

Cuts at:      0     365    1305 
   Size:        365     940
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     181     922    1305 
   Size:        181     741     383

  Fragments arranged by size: 

                 741     383     181
 
TatI w'GTAC_w

Cuts at:      0     500    1305 
   Size:        500     805
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     115     147    1305 
   Size:        115      32    1158

  Fragments arranged by size: 

                1158     115      32
 
XmaI C'CCGG_G

Cuts at:      0     862    1305 
   Size:        862     443
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AlfI     ApaI     AvaI     BanI    BanII
     BbeI     BbsI    BbvCI     BglI    BglII Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI
    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstXI    BstYI     BtgI
    BtgZI   DraIII     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I     FalI     FspI    FspAI    HaeII    Hin4I     KasI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NheI     NspI     PasI
     PmlI    PpuMI    PshAI   PspOMI     PsrI     PstI    PvuII    SacII
    SanDI     ScaI     SfcI     SfiI     SfoI     SmaI     SmlI     StyI
    TaqII     TatI     TstI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI     BlpI
     BplI    BsaBI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI
    BsrGI    BstBI   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI     DrdI  EcoICRI    EcoRI    EcoRV     FseI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     NcoI     NdeI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PpiI
     PsiI    PspXI     PvuI    RsrII     SacI     SalI     SapI     SbfI
    SexAI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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