Restriction map for pF1KB9856
 (Linear) (Six-base) MAPSORT of: KIBB9856.seq  Check: 3515  from: 1  to: 1170



KIBB9856.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AccI GT'mk_AC

Cuts at:      0     629    1170 
   Size:        629     541
 
AfeI AGC'GCT

Cuts at:      0     748    1170 
   Size:        748     422
 
AleI CACnn'nnGTG

Cuts at:      0     419    1170 
   Size:        419     751
 
ApaLI G'TGCA_C

Cuts at:      0    1161    1170 
   Size:       1161       9
 
AvaI C'yCGr_G

Cuts at:      0     755     781    1170 
   Size:        755      26     389

  Fragments arranged by size: 

                 755     389      26
 
AvrII C'CTAG_G

Cuts at:      0     945    1170 
   Size:        945     225
 
BanII G_rGCy'C

Cuts at:      0      46     762    1170 
   Size:         46     716     408

  Fragments arranged by size: 

                 716     408      46
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     516    1170 
   Size:        516     654
 
BglI GCCn_nnn'nGGC

Cuts at:      0     271    1170 
   Size:        271     899
 
Bme1580I G_kGCm'C

Cuts at:      0     833    1165    1170 
   Size:        833     332       5

  Fragments arranged by size: 

                 833     332       5
 
BmgBI CAC'GTC

Cuts at:      0     791     828    1170 
   Size:        791      37     342

  Fragments arranged by size: 

                 791     342      37
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     358     453    1170 
   Size:        358      95     717

  Fragments arranged by size: 

                 717     358      95
 
Bpu10I CC'TnA_GC

Cuts at:      0     569    1170 
   Size:        569     601
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47    1170 
   Size:         47    1123
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      43    1170 
   Size:         43    1127
 
BsaAI yAC'GTr

Cuts at:      0     696    1170 
   Size:        696     474
 
BsaBI GATnn'nnATC

Cuts at:      0     310    1012    1170 
   Size:        310     702     158

  Fragments arranged by size: 

                 702     310     158
 
BsaHI Gr'CG_yC

Cuts at:      0     458     561     727     887    1170 
   Size:        458     103     166     160     283

  Fragments arranged by size: 

                 458     283     166     160     103
 
BsaWI w'CCGG_w

Cuts at:      0     224     841    1170 
   Size:        224     617     329

  Fragments arranged by size: 

                 617     329     224
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     109     115     139     145    1170 
   Size:        109       6      24       6    1025

  Fragments arranged by size: 

                1025     109      24       6       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     595     658    1170 
   Size:        595      63     512

  Fragments arranged by size: 

                 595     512      63
 
BseYI C'CCAG_C

Cuts at:      0     761     832    1068    1170 
   Size:        761      71     236     102

  Fragments arranged by size: 

                 761     236     102      71
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     397    1170 
   Size:        223     174     773

  Fragments arranged by size: 

                 773     223     174
 
BsiEI CG_ry'CG

Cuts at:      0     339    1170 
   Size:        339     831
 
BsiHKAI G_wGCw'C

Cuts at:      0      18    1165    1170 
   Size:         18    1147       5

  Fragments arranged by size: 

                1147      18       5
 
BsmI GAATG_Cn'

Cuts at:      0     598    1135    1170 
   Size:        598     537      35

  Fragments arranged by size: 

                 598     537      35
 
Bsp1286I G_dGCh'C

Cuts at:      0      18      46     762     833    1165    1170 
   Size:         18      28     716      71     332       5

  Fragments arranged by size: 

                 716     332      71      28      18       5
 
BspEI T'CCGG_A

Cuts at:      0     841    1170 
   Size:        841     329
 
BspHI T'CATG_A

Cuts at:      0     503    1170 
   Size:        503     667
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     293    1170 
   Size:        293     877
 
BsrBI CCG'CTC

Cuts at:      0     707    1170 
   Size:        707     463
 
BsrFI r'CCGG_y

Cuts at:      0     334     739    1170 
   Size:        334     405     431

  Fragments arranged by size: 

                 431     405     334
 
BsrGI T'GTAC_A

Cuts at:      0     323    1170 
   Size:        323     847
 
BstEII G'GTnAC_C

Cuts at:      0     276     798    1170 
   Size:        276     522     372

  Fragments arranged by size: 

                 522     372     276
 
BstYI r'GATC_y

Cuts at:      0     844    1170 
   Size:        844     326
 
BtgI C'CryG_G

Cuts at:      0      38     461     635    1170 
   Size:         38     423     174     535

  Fragments arranged by size: 

                 535     423     174      38
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     411     657    1170 
   Size:        411     246     513

  Fragments arranged by size: 

                 513     411     246
 
BtsI GCAGTG_nn'

Cuts at:      0     398    1170 
   Size:        398     772
 
DraI TTT'AAA

Cuts at:      0    1128    1170 
   Size:       1128      42
 
EaeI y'GGCC_r

Cuts at:      0     182     336     684     914    1170 
   Size:        182     154     348     230     256

  Fragments arranged by size: 

                 348     256     230     182     154
 
EagI C'GGCC_G

Cuts at:      0     336    1170 
   Size:        336     834
 
EarI CTCTTCn'nnn_

Cuts at:      0     572    1170 
   Size:        572     598
 
HaeII r_GCGC'y

Cuts at:      0     104     172     750    1170 
   Size:        104      68     578     420

  Fragments arranged by size: 

                 578     420     104      68
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     115     147     252     284     571     603    1170 
   Size:        115      32     105      32     287      32     567

  Fragments arranged by size: 

                 567     287     115     105      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     362     569     908    1170 
   Size:        362     207     339     262

  Fragments arranged by size: 

                 362     339     262     207
 
MscI TGG'CCA

Cuts at:      0     184    1170 
   Size:        184     986
 
MslI CAynn'nnrTG

Cuts at:      0     301     419    1170 
   Size:        301     118     751

  Fragments arranged by size: 

                 751     301     118
 
MspA1I CmG'CkG

Cuts at:      0      40     341     765    1170 
   Size:         40     301     424     405

  Fragments arranged by size: 

                 424     405     301      40
 
PfoI T'CCnGG_A

Cuts at:      0     709    1170 
   Size:        709     461
 
PstI C_TGCA'G

Cuts at:      0     100    1170 
   Size:        100    1070
 
PvuII CAG'CTG

Cuts at:      0     765    1170 
   Size:        765     405
 
SacII CC_GC'GG

Cuts at:      0      41    1170 
   Size:         41    1129
 
ScaI AGT'ACT

Cuts at:      0     149     469    1170 
   Size:        149     320     701

  Fragments arranged by size: 

                 701     320     149
 
SfcI C'TryA_G

Cuts at:      0      96    1170 
   Size:         96    1074
 
SmaI CCC'GGG

Cuts at:      0     783    1170 
   Size:        783     387
 
SmlI C'TyrA_G

Cuts at:      0      62    1170 
   Size:         62    1108
 
SnaBI TAC'GTA

Cuts at:      0     696    1170 
   Size:        696     474
 
StyI C'CwwG_G

Cuts at:      0     104     179     945    1170 
   Size:        104      75     766     225

  Fragments arranged by size: 

                 766     225     104      75
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     631     786    1170 
   Size:        631     155     384

  Fragments arranged by size: 

                 631     384     155
 
TatI w'GTAC_w

Cuts at:      0     147     323     467    1170 
   Size:        147     176     144     703

  Fragments arranged by size: 

                 703     176     147     144
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     836     868    1170 
   Size:        836      32     302

  Fragments arranged by size: 

                 836     302      32
 
XmaI C'CCGG_G

Cuts at:      0     781    1170 
   Size:        781     389
 

 Enzymes that do cut:

     AccI     AfeI     AleI    ApaLI     AvaI    AvrII    BanII     BbsI
     BglI Bme1580I    BmgBI     BmrI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BstEII    BstYI     BtgI    BtgZI     BtsI     DraI     EaeI
     EagI     EarI    HaeII    Hin4I     MmeI     MscI     MslI   MspA1I
     PfoI     PstI    PvuII    SacII     ScaI     SfcI     SmaI     SmlI
    SnaBI     StyI    TaqII     TatI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI     AseI
    AsiSI     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI     BmtI     BplI     BpmI    BsiWI
    BsmBI    BsrDI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII     DrdI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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