Restriction map for pF1KB9846
 (Linear) (Six-base) MAPSORT of: KIBB9846.seq  Check: 4966  from: 1  to: 1122



KIBB9846.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AatII G_ACGT'C

Cuts at:      0     272    1122 
   Size:        272     850
 
AclI AA'CG_TT

Cuts at:      0     849    1122 
   Size:        849     273
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625     850    1122 
   Size:        625     225     272

  Fragments arranged by size: 

                 625     272     225
 
AfeI AGC'GCT

Cuts at:      0     583     806    1122 
   Size:        583     223     316

  Fragments arranged by size: 

                 583     316     223
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     659     691    1122 
   Size:        659      32     431

  Fragments arranged by size: 

                 659     431      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      21      74    1122 
   Size:         21      53    1048

  Fragments arranged by size: 

                1048      53      21
 
ApaI G_GGCC'C

Cuts at:      0     306    1122 
   Size:        306     816
 
ApaLI G'TGCA_C

Cuts at:      0     427    1122 
   Size:        427     695
 
ApoI r'AATT_y

Cuts at:      0     729    1122 
   Size:        729     393
 
AvaI C'yCGr_G

Cuts at:      0     307     354    1122 
   Size:        307      47     768

  Fragments arranged by size: 

                 768     307      47
 
BanII G_rGCy'C

Cuts at:      0     306    1122 
   Size:        306     816
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     496    1122 
   Size:        496     626
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       7      41    1122 
   Size:          7      34    1081

  Fragments arranged by size: 

                1081      34       7
 
Bme1580I G_kGCm'C

Cuts at:      0     306     431     908    1122 
   Size:        306     125     477     214

  Fragments arranged by size: 

                 477     306     214     125
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     849    1122 
   Size:        849     273
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     313     454     532     710    1006    1122 
   Size:        193     120     141      78     178     296     116

  Fragments arranged by size: 

                 296     193     178     141     120     116      78
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      36    1122 
   Size:         36    1086
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      54     982    1102    1122 
   Size:         54     928     120      20

  Fragments arranged by size: 

                 928     120      54      20
 
BsaHI Gr'CG_yC

Cuts at:      0     269     359     368    1122 
   Size:        269      90       9     754

  Fragments arranged by size: 

                 754     269      90       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     391     928    1122 
   Size:        391     537     194

  Fragments arranged by size: 

                 537     391     194
 
BseYI C'CCAG_C

Cuts at:      0     765     801    1122 
   Size:        765      36     321

  Fragments arranged by size: 

                 765     321      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     355    1122 
   Size:        169     186     767

  Fragments arranged by size: 

                 767     186     169
 
BsiHKAI G_wGCw'C

Cuts at:      0     431    1122 
   Size:        431     691
 
Bsp1286I G_dGCh'C

Cuts at:      0     306     431     908    1122 
   Size:        306     125     477     214

  Fragments arranged by size: 

                 477     306     214     125
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     128    1122 
   Size:        128     994
 
BsrBI CCG'CTC

Cuts at:      0     177    1122 
   Size:        177     945
 
BsrFI r'CCGG_y

Cuts at:      0     413    1122 
   Size:        413     709
 
BssHII G'CGCG_C

Cuts at:      0     112    1122 
   Size:        112    1010
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     435    1122 
   Size:        435     687
 
BtsI GCAGTG_nn'

Cuts at:      0     112    1122 
   Size:        112    1010
 
DraIII CAC_nnn'GTG

Cuts at:      0     333    1122 
   Size:        333     789
 
EarI CTCTTCn'nnn_

Cuts at:      0     653    1064    1122 
   Size:        653     411      58

  Fragments arranged by size: 

                 653     411      58
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     514    1122 
   Size:        514     608
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     313     454     532     625     710     850    1006 
   Size:        193     120     141      78      93      85     140     156

Cuts at:   1006    1122 
   Size:        116

  Fragments arranged by size: 

                 193     156     141     140     120     116      93      85
                  78
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     943    1122 
   Size:        943     179
 
EcoO109I rG'GnC_Cy

Cuts at:      0      78     252     303     948    1122 
   Size:         78     174      51     645     174

  Fragments arranged by size: 

                 645     174     174      78      51
 
EcoRI G'AATT_C

Cuts at:      0     729    1122 
   Size:        729     393
 
HaeII r_GCGC'y

Cuts at:      0     141     585     625     808    1122 
   Size:        141     444      40     183     314

  Fragments arranged by size: 

                 444     314     183     141      40
 
HincII GTy'rAC

Cuts at:      0     847    1122 
   Size:        847     275
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     644     864    1122 
   Size:        644     220     258

  Fragments arranged by size: 

                 644     258     220
 
NaeI GCC'GGC

Cuts at:      0     415    1122 
   Size:        415     707
 
NgoMIV G'CCGG_C

Cuts at:      0     413    1122 
   Size:        413     709
 
NspI r_CATG'y

Cuts at:      0     435    1122 
   Size:        435     687
 
PasI CC'CwG_GG

Cuts at:      0     908    1122 
   Size:        908     214
 
PfoI T'CCnGG_A

Cuts at:      0     231     362     470    1122 
   Size:        231     131     108     652

  Fragments arranged by size: 

                 652     231     131     108
 
PpuMI rG'GwC_Cy

Cuts at:      0      78    1122 
   Size:         78    1044
 
PshAI GACnn'nnGTC

Cuts at:      0     842    1122 
   Size:        842     280
 
PspOMI G'GGCC_C

Cuts at:      0     302    1122 
   Size:        302     820
 
PstI C_TGCA'G

Cuts at:      0      17     524    1122 
   Size:         17     507     598

  Fragments arranged by size: 

                 598     507      17
 
SanDI GG'GwC_CC

Cuts at:      0      78    1122 
   Size:         78    1044
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1064    1122 
   Size:       1064      58
 
SexAI A'CCwGG_T

Cuts at:      0     329    1023    1122 
   Size:        329     694      99

  Fragments arranged by size: 

                 694     329      99
 
SfcI C'TryA_G

Cuts at:      0      13     450     520    1122 
   Size:         13     437      70     602

  Fragments arranged by size: 

                 602     437      70      13
 
SgrAI Cr'CCGG_yG

Cuts at:      0     413    1122 
   Size:        413     709
 
SmaI CCC'GGG

Cuts at:      0     356    1122 
   Size:        356     766
 
SmlI C'TyrA_G

Cuts at:      0      51    1122 
   Size:         51    1071
 
SrfI GCCC'GGGC

Cuts at:      0     356    1122 
   Size:        356     766
 
StuI AGG'CCT

Cuts at:      0    1109    1122 
   Size:       1109      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     247     815    1122 
   Size:        247     568     307

  Fragments arranged by size: 

                 568     307     247
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     139     685    1122 
   Size:        139     546     437

  Fragments arranged by size: 

                 546     437     139
 
XmaI C'CCGG_G

Cuts at:      0     354    1122 
   Size:        354     768
 
XmnI GAAnn'nnTTC

Cuts at:      0       8     598    1122 
   Size:          8     590     524

  Fragments arranged by size: 

                 590     524       8
 
ZraI GAC'GTC

Cuts at:      0     270    1122 
   Size:        270     852
 

 Enzymes that do cut:

    AatII     AclI     AcuI     AfeI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AvaI    BanII     BbsI     BcgI Bme1580I     BmrI     BpmI
    BpuEI     BsaI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BsrFI   BssHII   BstAPI     BtsI   DraIII     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII   HincII     MmeI
     NaeI   NgoMIV     NspI     PasI     PfoI    PpuMI    PshAI   PspOMI
     PstI    SanDI     SapI    SexAI     SfcI    SgrAI     SmaI     SmlI
     SrfI     StuI    TaqII     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaWI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI     DrdI     EaeI     EagI  EcoICRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SbfI     ScaI     SfiI
     SfoI     SgfI    SnaBI     SpeI     SphI     SspI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI
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