Restriction map for pF1KB9841
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BseAI       BsiHKAI     BsiWI       BsmI        BsoBI       
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BssHII      Bst1107I    
BstBI       BstYI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO109I    
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        HaeII       HgaI        HindIII     HpaI        
KasI        KpnI        MaeI        MamI        MfeI        
MflI        MluI        MroI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SalI        
SapI        SbfI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SpeI        Sse8387I    SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII        1025  GACGTC               GACGT^C
AccII         722  CGCG                 CG^CG
AcyI         1025  GACGTC               G[GA]^CG[CT]C
AlwI          474  GATCC                GGATC(4/5)
AocI          961  CCTGAGG              CC^T[ACGT]AGG
Asp700I       612  GAATGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         348  GGTCC                G^G[AT]CC
AviII         299  TGCGCA               TGC^GCA
BalI          951  TGGCCA               TGG^CCA
BanI          966  GGTGCC               G^G[CT][GA]CC
BanII         354  GGGCTC               G[GA]GC[CT]^C
BciVI         592  GTATCC               GTATCC(6/5)
BglI          331  GCCGAGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         589  TACGTA               [CT]AC^GT[GA]
BsaHI        1025  GACGTC               G[GA]^CG[CT]C
BsaWI         155  TCCGGT               [AT]^CCGG[AT]
BseRI         655  CTCCTC               GAGGAG(10/8)
BsiEI          53  CGACCG               CG[GA][CT]^CG
BsmBI         252  CGTCTC               CGTCTC(1/5)
Bsp1407I      272  TGTACA               T^GTACA
BsrGI         272  TGTACA               T^GTACA
BssSI         103  CTCGTG               CACGAG(-5/-1)
BstAPI        291  GCACACTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         722  CGCG                 CG^CG
Bsu36I        961  CCTGAGG              CC^T[ACGT]AGG
DraIII        292  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          880  GACAACCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          951  TGGCCA               [CT]^GGCC[GA]
EarI           85  CTCTTC               CTCTTC(1/4)
Eco81I        961  CCTGAGG              CC^T[ACGT]AGG
EcoNI         922  CCTGGGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI          299  TGCGCA               TGC^GCA
Hin1I        1025  GACGTC               G[GA]^CG[CT]C
HincII        580  GTCAAC               GT[CT]^[GA]AC
HindII        580  GTCAAC               GT[CT]^[GA]AC
Ksp632I        85  CTCTTC               CTCTTC(1/4)
KspI          721  CCGCGG               CCGC^GG
MluNI         951  TGGCCA               TGG^CCA
MscI          951  TGGCCA               TGG^CCA
MseI          800  TTAA                 T^TAA
MvnI          722  CGCG                 CG^CG
PstI          238  CTGCAG               CTGCA^G
PvuII         116  CAGCTG               CAG^CTG
SacII         721  CCGCGG               CCGC^GG
ScaI          512  AGTACT               AGT^ACT
SfaNI         467  GCATC                GCATC(5/9)
SfcI          238  CTGCAG               C^T[GA][CT]AG
SfiI          330  GGCCGAGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI         589  TACGTA               TAC^GTA
SphI          302  GCATGC               GCATG^C
SspBI         272  TGTACA               T^GTACA
TaqI           52  TCGA                 T^CGA
Tru9I         800  TTAA                 T^TAA
TthHB8I        52  TCGA                 T^CGA
XmnI          612  GAATGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI           24  AAATTT               [GA]^AATT[CT]
              832  AAATTC              
AlwNI         116  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              805  CAGTGCCTG           
ApoI           24  AAATTT               [GA]^AATT[CT]
              832  AAATTC              
BbsI          126  GTCTTC               GAAGAC(2/6)
              616  GTCTTC              
BpuAI         126  GTCTTC               GAAGAC(2/6)
              616  GTCTTC              
BsmAI         253  GTCTC                GTCTC(1/5)
              979  GTCTC               
BsrI          230  ACTGG                ACTGG(1/-1)
              510  CCAGT               
BstEII        888  GGTCACC              G^GT[ACGT]ACC
              988  GGTCACC             
BstPI         888  GGTCACC              G^GT[ACGT]ACC
              988  GGTCACC             
BstXI         485  CCATGGAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              744  CCAGCTTTCTGG        
CfoI          300  GCGC                 GCG^C
              315  GCGC                
EcoO65I       888  GGTCACC              G^GT[ACGT]ACC
              988  GGTCACC             
EcoT14I       485  CCATGG               C^C[AT][AT]GG
             1002  CCATGG              
HhaI          300  GCGC                 GCG^C
              315  GCGC                
HinP1I        300  GCGC                 G^CGC
              315  GCGC                
MaeII         590  ACGT                 A^CGT
             1026  ACGT                
MspA1I        116  CAGCTG               C[AC]G^C[GT]G
              721  CCGCGG              
NcoI          485  CCATGG               C^CATGG
             1002  CCATGG              
NlaIV         754  GGCCCC               GG[ACGT]^[ACGT]CC
              966  GGTGCC              
NspI          302  GCATGC               [GA]CATG^[CT]
              546  GCATGT              
SexAI         884  ACCTGGT              A^CC[AT]GGT
              984  ACCAGGT             
StyI          485  CCATGG               C^C[AT][AT]GG
             1002  CCATGG              
TfiI          168  GATTC                G^A[AT]TC
              527  GAATC               
Tsp509I        25  AATT                 ^AATT
              833  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BcnI        
BmyI        BpmI        BsaJI       BsgI        BsiYI       
BslI        BsmFI       Bsp1286I    BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DsaI        EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MnlI        MslI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      PleI        RsaI        Sau3AI      Sau96I      
ScrFI       TseI        Tsp45I      TspRI       XcmI        
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