Restriction map for pF1KB9841
 (Linear) (Six-base) MAPSORT of: KIBB9841.seq  Check: 1822  from: 1  to: 1074



KIBB9841.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AatII G_ACGT'C

Cuts at:      0    1029    1074 
   Size:       1029      45
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     831    1074 
   Size:        831     243
 
AleI CACnn'nnGTG

Cuts at:      0     457    1074 
   Size:        457     617
 
AlwNI CAG_nnn'CTG

Cuts at:      0     121     810    1074 
   Size:        121     689     264

  Fragments arranged by size: 

                 689     264     121
 
ApoI r'AATT_y

Cuts at:      0      24     832    1074 
   Size:         24     808     242

  Fragments arranged by size: 

                 808     242      24
 
BanI G'GyrC_C

Cuts at:      0     966    1074 
   Size:        966     108
 
BanII G_rGCy'C

Cuts at:      0     358    1074 
   Size:        358     716
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     119     609    1074 
   Size:        119     490     465

  Fragments arranged by size: 

                 490     465     119
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     603    1074 
   Size:        603     471
 
BglI GCCn_nnn'nGGC

Cuts at:      0     337    1074 
   Size:        337     737
 
Bme1580I G_kGCm'C

Cuts at:      0     115     442     971    1074 
   Size:        115     327     529     103

  Fragments arranged by size: 

                 529     327     115     103
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     240    1074 
   Size:        240     834
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     418     832    1074 
   Size:         52     366     414     242

  Fragments arranged by size: 

                 414     366     242      52
 
Bpu10I CC'TnA_GC

Cuts at:      0     661    1074 
   Size:        661     413
 
BsaAI yAC'GTr

Cuts at:      0     591    1074 
   Size:        591     483
 
BsaHI Gr'CG_yC

Cuts at:      0    1026    1074 
   Size:       1026      48
 
BsaWI w'CCGG_w

Cuts at:      0     155    1074 
   Size:        155     919
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     552     582     638     668    1074 
   Size:        552      30      56      30     406

  Fragments arranged by size: 

                 552     406      56      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     646    1074 
   Size:        646     428
 
BseYI C'CCAG_C

Cuts at:      0     108     404    1074 
   Size:        108     296     670

  Fragments arranged by size: 

                 670     296     108
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     483     629     765    1074 
   Size:        483     146     136     309

  Fragments arranged by size: 

                 483     309     146     136
 
BsiEI CG_ry'CG

Cuts at:      0      56    1074 
   Size:         56    1018
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     258    1074 
   Size:        258     816
 
Bsp1286I G_dGCh'C

Cuts at:      0     115     358     442     971    1074 
   Size:        115     243      84     529     103

  Fragments arranged by size: 

                 529     243     115     103      84
 
BsrGI T'GTAC_A

Cuts at:      0     272    1074 
   Size:        272     802
 
BssSI C'ACGA_G

Cuts at:      0     103    1074 
   Size:        103     971
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     297    1074 
   Size:        297     777
 
BstEII G'GTnAC_C

Cuts at:      0     888     988    1074 
   Size:        888     100      86

  Fragments arranged by size: 

                 888     100      86
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     492     751    1074 
   Size:        492     259     323

  Fragments arranged by size: 

                 492     323     259
 
Bsu36I CC'TnA_GG

Cuts at:      0     962    1074 
   Size:        962     112
 
BtgI C'CryG_G

Cuts at:      0      56     485     721    1002    1074 
   Size:         56     429     236     281      72

  Fragments arranged by size: 

                 429     281     236      72      56
 
DraIII CAC_nnn'GTG

Cuts at:      0     297    1074 
   Size:        297     777
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     886    1074 
   Size:        886     188
 
EaeI y'GGCC_r

Cuts at:      0     951    1074 
   Size:        951     123
 
EarI CTCTTCn'nnn_

Cuts at:      0      91    1074 
   Size:         91     983
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     418     831     832    1074 
   Size:         52     366     413       1     242

  Fragments arranged by size: 

                 413     366     242      52       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     926    1074 
   Size:        926     148
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     633    1074 
   Size:        633     441
 
FspI TGC'GCA

Cuts at:      0     301    1074 
   Size:        301     773
 
FspAI rTGC'GCAy

Cuts at:      0     301    1074 
   Size:        301     773
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     552     584    1051    1074 
   Size:        552      32     467      23

  Fragments arranged by size: 

                 552     467      32      23
 
HincII GTy'rAC

Cuts at:      0     582    1074 
   Size:        582     492
 
MscI TGG'CCA

Cuts at:      0     953    1074 
   Size:        953     121
 
MslI CAynn'nnrTG

Cuts at:      0     457     484     490    1074 
   Size:        457      27       6     584

  Fragments arranged by size: 

                 584     457      27       6
 
MspA1I CmG'CkG

Cuts at:      0     118     723    1074 
   Size:        118     605     351

  Fragments arranged by size: 

                 605     351     118
 
NcoI C'CATG_G

Cuts at:      0     485    1002    1074 
   Size:        485     517      72

  Fragments arranged by size: 

                 517     485      72
 
NspI r_CATG'y

Cuts at:      0     306     550    1074 
   Size:        306     244     524

  Fragments arranged by size: 

                 524     306     244
 
PasI CC'CwG_GG

Cuts at:      0     823    1074 
   Size:        823     251
 
PfoI T'CCnGG_A

Cuts at:      0      29    1074 
   Size:         29    1045
 
PstI C_TGCA'G

Cuts at:      0     242    1074 
   Size:        242     832
 
PvuII CAG'CTG

Cuts at:      0     118    1074 
   Size:        118     956
 
SacII CC_GC'GG

Cuts at:      0     724    1074 
   Size:        724     350
 
ScaI AGT'ACT

Cuts at:      0     514    1074 
   Size:        514     560
 
SexAI A'CCwGG_T

Cuts at:      0     884     984    1074 
   Size:        884     100      90

  Fragments arranged by size: 

                 884     100      90
 
SfcI C'TryA_G

Cuts at:      0     238    1074 
   Size:        238     836
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     337    1074 
   Size:        337     737
 
SnaBI TAC'GTA

Cuts at:      0     591    1074 
   Size:        591     483
 
SphI G_CATG'C

Cuts at:      0     306    1074 
   Size:        306     768
 
StyI C'CwwG_G

Cuts at:      0     485    1002    1074 
   Size:        485     517      72

  Fragments arranged by size: 

                 517     485      72
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     222    1074 
   Size:        222     852
 
TatI w'GTAC_w

Cuts at:      0      39     272     512    1074 
   Size:         39     233     240     562

  Fragments arranged by size: 

                 562     240     233      39
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     638     670    1074 
   Size:        638      32     404

  Fragments arranged by size: 

                 638     404      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     264     489     946    1074 
   Size:        264     225     457     128

  Fragments arranged by size: 

                 457     264     225     128
 
XmnI GAAnn'nnTTC

Cuts at:      0     616    1074 
   Size:        616     458
 
ZraI GAC'GTC

Cuts at:      0    1027    1074 
   Size:       1027      47
 

 Enzymes that do cut:

    AatII     AcuI     AleI    AlwNI     ApoI     BanI    BanII     BbsI
    BciVI     BglI Bme1580I     BmrI     BpmI   Bpu10I    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsmBI Bsp1286I
    BsrGI    BssSI   BstAPI   BstEII    BstXI   Bsu36I     BtgI   DraIII
     DrdI     EaeI     EarI  Eco57MI    EcoNI     FalI     FspI    FspAI
    Hin4I   HincII     MscI     MslI   MspA1I     NcoI     NspI     PasI
     PfoI     PstI    PvuII    SacII     ScaI    SexAI     SfcI     SfiI
    SnaBI     SphI     StyI    TaqII     TatI     TstI     XcmI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI    BpuEI     BsaI
    BsaBI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BstBI    BstYI  BstZ17I    BtgZI     BtsI
     ClaI    CspCI     DraI     EagI     EciI  EcoICRI EcoO109I    EcoRI
    EcoRV     FseI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI     SalI    SanDI     SapI     SbfI     SfoI     SgfI    SgrAI
     SmaI     SmlI     SpeI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XhoI     XmaI
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