Restriction map for pF1KB9830
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccII       AciI        AclI        
AcyI        AflIII      AhdI        AluI        AlwNI       
AocI        Aor51HI     ApaI        AscI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaHI       BsaI        BseRI       BsiWI       BsmBI       
Bsp1407I    BspLU11I    BspMI       BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CpoI        DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRV       EcoT14I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HindIII     KasI        KpnI        KspI        MfeI        
MluI        MluNI       MscI        MslI        MspA1I      
MunI        MvnI        MwoI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PstI        PvuII       RsrII       SacI        
SacII       SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SpeI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tth111I     Van91I      XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   74 enzymes) #
###################################

AccI           10  GTCGAC               GT^[AC][GT]AC
AccIII        158  TCCGGA               T^CCGGA
AfaI          648  GTAC                 GT^AC
AflII         187  CTTAAG               C^TTAAG
AgeI          650  ACCGGT               A^CCGGT
Alw44I         48  GTGCAC               G^TGCAC
ApaLI          48  GTGCAC               G^TGCAC
AseI          598  ATTAAT               AT^TAAT
AsnI          598  ATTAAT               AT^TAAT
AvaI          791  CCCGGG               C^[CT]CG[GA]G
BamHI         879  GGATCC               G^GATCC
BbsI          372  GAAGAC               GAAGAC(2/6)
BbvI          495  GCAGC                GCAGC(8/12)
BfaI          550  CTAG                 C^TAG
BfrI          187  CTTAAG               C^TTAAG
BpuAI         372  GAAGAC               GAAGAC(2/6)
BsaAI         658  TACGTA               [CT]AC^GT[GA]
BsaBI         351  GATAACTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         158  TCCGGA               T^CCGGA
BsgI          807  CTGCAC               GTGCAG(16/14)
BsiEI         360  CGATCG               CG[GA][CT]^CG
BsiYI         786  CCAAACCCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          786  CCAAACCCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          448  GCATTC               GAATGC(1/-1)
BsoBI         791  CCCGGG               C^[CT]CG[GA]G
BspDI         358  ATCGAT               AT^CGAT
BspEI         158  TCCGGA               T^CCGGA
BspHI         215  TCATGA               T^CATGA
BsrDI         723  CATTGC               GCAATG(2/0)
BsrFI         650  ACCGGT               [GA]^CCGG[CT]
BstEII       1000  GGTGACC              G^GT[ACGT]ACC
BstPI        1000  GGTGACC              G^GT[ACGT]ACC
BstYI         879  GGATCC               [GA]^GATC[CT]
CfoI           28  GCGC                 GCG^C
Cfr10I        650  ACCGGT               [GA]^CCGG[CT]
ClaI          358  ATCGAT               AT^CGAT
DraI          388  TTTAAA               TTT^AAA
DraII          74  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DraIII        846  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I       74  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1000  GGTGACC              G^GT[ACGT]ACC
EcoRI         767  GAATTC               G^AATTC
EcoT22I       869  ATGCAT               ATGCA^T
Fnu4HI        495  GCAGC                GC^[ACGT]GC
HhaI           28  GCGC                 GCG^C
HinP1I         28  GCGC                 G^CGC
HpaI          855  GTTAAC               GTT^AAC
ItaI          495  GCAGC                GC^[ACGT]GC
MaeI          550  CTAG                 C^TAG
MamI          351  GATAACTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          879  GGATCC               [GA]^GATC[CT]
MroI          158  TCCGGA               T^CCGGA
NlaIV         879  GGATCC               GG[ACGT]^[ACGT]CC
NsiI          869  ATGCAT               ATGCA^T
NspI          871  GCATGC               [GA]CATG^[CT]
PinAI         650  ACCGGT               A^CCGGT
PleI         1017  GACTC                GAGTC(4/5)
PpuMI          74  AGGTCCT              [GA]G^G[AT]CC[CT]
PshBI         598  ATTAAT               AT^TAAT
PvuI          360  CGATCG               CGAT^CG
RcaI          215  TCATGA               T^CATGA
RsaI          648  GTAC                 GT^AC
SalI           10  GTCGAC               G^TCGAC
SfaNI         250  GATGC                GCATC(5/9)
SmaI          791  CCCGGG               CCC^GGG
SmlI          187  CTTAAG               C^T[CT][GA]AG
SnaBI         658  TACGTA               TAC^GTA
SphI          871  GCATGC               GCATG^C
TseI          495  GCAGC                G^C[AT]GC
Tsp45I       1001  GTGAC                ^GT[GC]AC
XcmI          404  CCAAAAATCTCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         879  GGATCC               [GA]^GATC[CT]
XmaCI         791  CCCGGG               C^CCGGG
XmaI          791  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwI          880  GATCC                GGATC(4/5)
              879  GGATC               
AspHI          48  GTGCAC               G[AT]GC[AT]^C
              843  GAGCAC              
AvaII          75  GGTCC                G^G[AT]CC
              268  GGACC               
BcnI          791  CCCGG                CC^[GC]GG
              792  CCGGG               
BsaWI         158  TCCGGA               [AT]^CCGG[AT]
              650  ACCGGT              
BsiHKAI        48  GTGCAC               G[AT]GC[AT]^C
              843  GAGCAC              
BsrI          258  ACTGG                ACTGG(1/-1)
               41  CCAGT               
Cac8I           3  GCAAGC               GC[ACGT]^[ACGT]GC
              871  GCATGC              
Cfr13I         75  GGTCC                G^G[ACGT]CC
              268  GGACC               
EarI           79  CTCTTC               CTCTTC(1/4)
             1012  GAAGAG              
HaeIII        130  GGCC                 GG^CC
              383  GGCC                
HincII         10  GTCGAC               GT[CT]^[GA]AC
              855  GTTAAC              
HindII         10  GTCGAC               GT[CT]^[GA]AC
              855  GTTAAC              
Ksp632I        79  CTCTTC               CTCTTC(1/4)
             1012  GAAGAG              
MaeII         659  ACGT                 A^CGT
              704  ACGT                
MaeIII        295  GTTAC                ^GT[ACGT]AC
             1001  GTGAC               
NciI          791  CCCGG                CC^[GC]GG
              792  CCGGG               
Sau96I         75  GGTCC                G^G[ACGT]CC
              268  GGACC               
TaqI           11  TCGA                 T^CGA
              359  TCGA                
TthHB8I        11  TCGA                 T^CGA
              359  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AcsI        ApoI        BmyI        BsaJI       BsmAI       
BsmFI       Bsp1286I    BssKI       BstF5I      BstNI       
DdeI        DpnII       EcoRII      FokI        HapII       
HinfI       HpaII       HphI        MboI        MboII       
MnlI        MseI        MspI        MvaI        NdeII       
NlaIII      Sau3AI      ScrFI       TfiI        Tru9I       
Tsp509I     TspRI       
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