Restriction map for pF1KB9830
 (Linear) (Six-base) MAPSORT of: KIBB9830.seq  Check: 865  from: 1  to: 1026



KIBB9830.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AccI GT'mk_AC

Cuts at:      0      11    1026 
   Size:         11    1015
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     897     958    1026 
   Size:        897      61      68

  Fragments arranged by size: 

                 897      68      61
 
AflII C'TTAA_G

Cuts at:      0     187    1026 
   Size:        187     839
 
AgeI A'CCGG_T

Cuts at:      0     650    1026 
   Size:        650     376
 
ApaLI G'TGCA_C

Cuts at:      0      48    1026 
   Size:         48     978
 
ApoI r'AATT_y

Cuts at:      0     239     767     918    1026 
   Size:        239     528     151     108

  Fragments arranged by size: 

                 528     239     151     108
 
AseI AT'TA_AT

Cuts at:      0     599    1026 
   Size:        599     427
 
AvaI C'yCGr_G

Cuts at:      0     791    1026 
   Size:        791     235
 
BamHI G'GATC_C

Cuts at:      0     879    1026 
   Size:        879     147
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     379    1026 
   Size:        379     647
 
BfrBI ATG'CAT

Cuts at:      0     871    1026 
   Size:        871     155
 
Bme1580I G_kGCm'C

Cuts at:      0      52     946    1026 
   Size:         52     894      80

  Fragments arranged by size: 

                 894      80      52
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     268    1026 
   Size:        268     758
 
Bpu10I CC'TnA_GC

Cuts at:      0     781    1026 
   Size:        781     245
 
BsaAI yAC'GTr

Cuts at:      0     660    1026 
   Size:        660     366
 
BsaBI GATnn'nnATC

Cuts at:      0     355    1026 
   Size:        355     671
 
BsaWI w'CCGG_w

Cuts at:      0     158     650    1026 
   Size:        158     492     376

  Fragments arranged by size: 

                 492     376     158
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     792    1026 
   Size:        792     234
 
BsiEI CG_ry'CG

Cuts at:      0     363    1026 
   Size:        363     663
 
BsiHKAI G_wGCw'C

Cuts at:      0      52     847    1026 
   Size:         52     795     179

  Fragments arranged by size: 

                 795     179      52
 
BsmI GAATG_Cn'

Cuts at:      0     448    1026 
   Size:        448     578
 
Bsp1286I G_dGCh'C

Cuts at:      0      52     847     946    1026 
   Size:         52     795      99      80

  Fragments arranged by size: 

                 795      99      80      52
 
BspEI T'CCGG_A

Cuts at:      0     158    1026 
   Size:        158     868
 
BspHI T'CATG_A

Cuts at:      0     215    1026 
   Size:        215     811
 
BsrDI GCAATG_nn'

Cuts at:      0     722    1026 
   Size:        722     304
 
BsrFI r'CCGG_y

Cuts at:      0     650    1026 
   Size:        650     376
 
BstEII G'GTnAC_C

Cuts at:      0    1000    1026 
   Size:       1000      26
 
BstYI r'GATC_y

Cuts at:      0     879    1026 
   Size:        879     147
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     151    1026 
   Size:        151     875
 
BtsI GCAGTG_nn'

Cuts at:      0      85     809    1026 
   Size:         85     724     217

  Fragments arranged by size: 

                 724     217      85
 
ClaI AT'CG_AT

Cuts at:      0     359    1026 
   Size:        359     667
 
DraI TTT'AAA

Cuts at:      0     390    1026 
   Size:        390     636
 
DraIII CAC_nnn'GTG

Cuts at:      0     851    1026 
   Size:        851     175
 
EarI CTCTTCn'nnn_

Cuts at:      0      85    1007    1026 
   Size:         85     922      19

  Fragments arranged by size: 

                 922      85      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     897     958    1026 
   Size:        897      61      68

  Fragments arranged by size: 

                 897      68      61
 
EcoO109I rG'GnC_Cy

Cuts at:      0      75    1026 
   Size:         75     951
 
EcoRI G'AATT_C

Cuts at:      0     767    1026 
   Size:        767     259
 
HincII GTy'rAC

Cuts at:      0      12     857    1026 
   Size:         12     845     169

  Fragments arranged by size: 

                 845     169      12
 
HpaI GTT'AAC

Cuts at:      0     857    1026 
   Size:        857     169
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     422    1026 
   Size:         93     329     604

  Fragments arranged by size: 

                 604     329      93
 
NsiI A_TGCA'T

Cuts at:      0     873    1026 
   Size:        873     153
 
NspI r_CATG'y

Cuts at:      0     875    1026 
   Size:        875     151
 
PpuMI rG'GwC_Cy

Cuts at:      0      75    1026 
   Size:         75     951
 
PvuI CG_AT'CG

Cuts at:      0     363    1026 
   Size:        363     663
 
SalI G'TCGA_C

Cuts at:      0      10    1026 
   Size:         10    1016
 
SmaI CCC'GGG

Cuts at:      0     793    1026 
   Size:        793     233
 
SmlI C'TyrA_G

Cuts at:      0     187    1026 
   Size:        187     839
 
SnaBI TAC'GTA

Cuts at:      0     660    1026 
   Size:        660     366
 
SphI G_CATG'C

Cuts at:      0     875    1026 
   Size:        875     151
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      85     535    1026 
   Size:         85     450     491

  Fragments arranged by size: 

                 491     450      85
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     411    1026 
   Size:        411     615
 
XmaI C'CCGG_G

Cuts at:      0     791    1026 
   Size:        791     235
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AgeI    ApaLI     ApoI     AseI     AvaI
    BamHI     BbsI    BfrBI Bme1580I     BmrI   Bpu10I    BsaAI    BsaBI
    BsaWI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BsrDI    BsrFI   BstEII    BstYI    BtgZI     BtsI     ClaI     DraI
   DraIII     EarI  Eco57MI EcoO109I    EcoRI   HincII     HpaI     MmeI
     NsiI     NspI    PpuMI     PvuI     SalI     SmaI     SmlI    SnaBI
     SphI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI     AscI    AsiSI    AvrII     BaeI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaHI    BsaXI    BseRI    BseYI    BsiWI    BsmBI    BspMI    BsrBI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I
     BtgI    CspCI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI    PvuII    RsrII     SacI
    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SpeI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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