Restriction map for pF1KB9822
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvrII       BalI        BamHI       BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsgI        BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrGI       BsrI        BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
HpaI        KpnI        MaeIII      MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MunI        NaeI        NdeI        NgoMIV      
NheI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SalI        SapI        SbfI        SexAI       
SfcI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Tsp45I      
TspRI       Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AcsI          364  GAATTC               [GA]^AATT[CT]
AlwNI          94  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          364  GAATTC               [GA]^AATT[CT]
AspHI         652  GAGCTC               G[AT]GC[AT]^C
AvaII         399  GGACC                G^G[AT]CC
BfaI          296  CTAG                 C^TAG
BsiEI         420  CGGCCG               CG[GA][CT]^CG
BsiHKAI       652  GAGCTC               G[AT]GC[AT]^C
BsmAI          88  GAGAC                GTCTC(1/5)
BsmBI          88  GAGACG               CGTCTC(1/5)
BsmFI          66  GTCCC                GGGAC(10/14)
BsrBI         501  GAGCGG               CCGCTC(-3/-3)
BsrDI         764  GCAATG               GCAATG(2/0)
BsrFI         818  GCCGGT               [GA]^CCGG[CT]
Cfr10I        818  GCCGGT               [GA]^CCGG[CT]
DdeI          141  CTTAG                C^T[ACGT]AG
DpnII         180  GATC                 ^GATC
EagI          420  CGGCCG               C^GGCCG
EarI          904  GAAGAG               CTCTTC(1/4)
EclXI         420  CGGCCG               C^GGCCG
Eco52I        420  CGGCCG               C^GGCCG
EcoRI         364  GAATTC               G^AATTC
HincII        448  GTCAAC               GT[CT]^[GA]AC
HindII        448  GTCAAC               GT[CT]^[GA]AC
HindIII       865  AAGCTT               A^AGCTT
Ksp632I       904  GAAGAG               CTCTTC(1/4)
MaeI          296  CTAG                 C^TAG
MaeII         879  ACGT                 A^CGT
MboI          180  GATC                 ^GATC
NcoI          227  CCATGG               C^CATGG
NdeII         180  GATC                 ^GATC
NlaIII        228  CATG                 CATG^
NotI          419  GCGGCCGC             GC^GGCCGC
SacI          652  GAGCTC               GAGCT^C
Sau3AI        180  GATC                 ^GATC
ScaI          389  AGTACT               AGT^ACT
SfaNI         434  GCATC                GCATC(5/9)
Tsp509I       365  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AfaI          390  GTAC                 GT^AC
              778  GTAC                
Aor51HI       131  AGCGCT               AGC^GCT
              689  AGCGCT              
AviII         431  TGCGCA               TGC^GCA
              525  TGCGCA              
BglI          608  GCCCGGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              629  GCCCAGGGGGC         
BseRI         328  GAGGAG               GAGGAG(10/8)
              919  GAGGAG              
BssHII        348  GCGCGC               G^CGCGC
              350  GCGCGC              
BstXI         227  CCATGGCGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              671  CCATCACCGTGG        
Eco47III      131  AGCGCT               AGC^GCT
              689  AGCGCT              
EcoT14I       161  CCAAGG               C^C[AT][AT]GG
              227  CCATGG              
FspI          431  TGCGCA               TGC^GCA
              525  TGCGCA              
MslI          228  CATGGCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              672  CATCACCGTG          
PvuII         286  CAGCTG               CAG^CTG
              310  CAGCTG              
RsaI          390  GTAC                 GT^AC
              778  GTAC                
SmaI          481  CCCGGG               CCC^GGG
              609  CCCGGG              
StyI          161  CCAAGG               C^C[AT][AT]GG
              227  CCATGG              
XmaCI         481  CCCGGG               C^CCGGG
              609  CCCGGG              
XmaI          481  CCCGGG               C^CCGGG
              609  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        ApaI        
AvaI        BanI        BanII       BbeI        BbvI        
BcnI        BmyI        BsaHI       BsaJI       BsiYI       
BslI        BsoBI       Bsp1286I    BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DraII       DsaI        EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
KspI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NlaIV       PleI        SacII       Sau96I      ScrFI       
SfoI        SmlI        TaqI        TseI        TthHB8I     
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