Restriction map for
pF1KB9822
(Circular) (Six-base) MAPSORT of: pF1KB9822.seq Check: 1684 from: 1 to: 4049
pF1KB9822 4049 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1087 1087
Size: 4049
Acc65I G'GTAC_C
Cuts at: 1065 1065
Size: 4049
AclI AA'CG_TT
Cuts at: 3889 3889
Size: 4049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1874 2306 3053 3321 1874
Size: 432 747 268 2602
Fragments arranged by size:
2602 747 432 268
AfeI AGC'GCT
Cuts at: 241 799 241
Size: 558 3491
AflIII A'CryG_T
Cuts at: 1401 2505 3482 1401
Size: 1104 977 1968
Fragments arranged by size:
1968 1104 977
AgeI A'CCGG_T
Cuts at: 2437 2437
Size: 4049
AlwNI CAG_nnn'CTG
Cuts at: 207 1354 2921 207
Size: 1147 1567 1335
Fragments arranged by size:
1567 1335 1147
ApaI G_GGCC'C
Cuts at: 403 589 739 403
Size: 186 150 3713
Fragments arranged by size:
3713 186 150
ApaLI G'TGCA_C
Cuts at: 2819 2819
Size: 4049
ApoI r'AATT_y
Cuts at: 472 1053 3402 3658 472
Size: 581 2349 256 863
Fragments arranged by size:
2349 863 581 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 412 589 717 1069 412
Size: 177 128 352 3392
Fragments arranged by size:
3392 352 177 128
BamHI G'GATC_C
Cuts at: 1074 1074
Size: 4049
BanI G'GyrC_C
Cuts at: 430 727 772 916 1065 1479 1727 1762 430
Size: 297 45 144 149 414 248 35 2717
Fragments arranged by size:
2717 414 297 248 149 144 45 35
BanII G_rGCy'C
Cuts at: 403 589 739 764 1063 2093 403
Size: 186 150 25 299 1030 2359
Fragments arranged by size:
2359 1030 299 186 150 25
BbeI G_GCGC'C
Cuts at: 434 731 920 1483 1731 434
Size: 297 189 563 248 2752
Fragments arranged by size:
2752 563 297 248 189
BciVI GTATCCnnnnn_n'
Cuts at: 1947 2708 4049 1947
Size: 761 1341 1947
Fragments arranged by size:
1947 1341 761
BclI T'GATC_A
Cuts at: 1569 1569
Size: 4049
BglI GCCn_nnn'nGGC
Cuts at: 722 743 722
Size: 21 4028
BglII A'GATC_T
Cuts at: 1564 3601 1564
Size: 2037 2012
BlpI GC'TnA_GC
Cuts at: 1161 1161
Size: 4049
Bme1580I G_kGCm'C
Cuts at: 403 589 739 775 1674 1767 2823 403
Size: 186 150 36 899 93 1056 1629
Fragments arranged by size:
1629 1056 899 186 150 93 36
BmgBI CAC'GTC
Cuts at: 988 988
Size: 4049
BmrI ACTGGGnnnn_n'
Cuts at: 1261 1342 1677 3821 1261
Size: 81 335 2144 1489
Fragments arranged by size:
2144 1489 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 228 792 2416 228
Size: 564 1624 1861
Fragments arranged by size:
1861 1624 564
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 448 496 1225 2104 2596 2894 268
Size: 180 48 729 879 492 298 1423
Fragments arranged by size:
1423 879 729 492 298 180 48
BsaI GGTCTCn'nnnn_
Cuts at: 3753 3753
Size: 4049
BsaAI yAC'GTr
Cuts at: 1402 1402
Size: 4049
BsaBI GATnn'nnATC
Cuts at: 6 1563 3600 6
Size: 1557 2037 455
Fragments arranged by size:
2037 1557 455
BsaHI Gr'CG_yC
Cuts at: 431 728 917 1480 1728 2430 3926 431
Size: 297 189 563 248 702 1496 554
Fragments arranged by size:
1496 702 563 554 297 248 189
BsaWI w'CCGG_w
Cuts at: 1238 1759 2437 2711 2858 3269 1238
Size: 521 678 274 147 411 2018
Fragments arranged by size:
2018 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 1042 451
Size: 591 3458
BseYI C'CCAG_C
Cuts at: 366 396 1474 2232 2809 3305 366
Size: 30 1078 758 577 496 1110
Fragments arranged by size:
1110 1078 758 577 496 30
BsiEI CG_ry'CG
Cuts at: 104 531 1591 1637 2845 104
Size: 427 1060 46 1208 1308
Fragments arranged by size:
1308 1208 1060 427 46
BsiHKAI G_wGCw'C
Cuts at: 764 1063 1841 2031 2823 764
Size: 299 778 190 792 1990
Fragments arranged by size:
1990 792 778 299 190
BsmBI CGTCTCn'nnnn_
Cuts at: 190 190
Size: 4049
Bsp1286I G_dGCh'C
Cuts at: 403 589 739 764 775 1063 1674 1767 1841
Size: 186 150 25 11 288 611 93 74
Cuts at: 1841 2031 2093 2823 403
Size: 190 62 730 1629
Fragments arranged by size:
1629 730 611 288 190 186 150 93
74 62 25 11
BspEI T'CCGG_A
Cuts at: 3269 3269
Size: 4049
BspHI T'CATG_A
Cuts at: 3225 3225
Size: 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1099 1313 1615 1996 1099
Size: 214 302 381 3152
Fragments arranged by size:
3152 381 302 214
BsrBI CCG'CTC
Cuts at: 50 611 2341 2395 3879 4044 50
Size: 561 1730 54 1484 165 55
Fragments arranged by size:
1730 1484 561 165 55 54
BsrDI GCAATG_nn'
Cuts at: 879 1961 879
Size: 1082 2967
BsrFI r'CCGG_y
Cuts at: 926 2047 2228 2437 926
Size: 1121 181 209 2538
Fragments arranged by size:
2538 1121 209 181
BssHII G'CGCG_C
Cuts at: 456 458 456
Size: 2 4047
BssSI C'ACGA_G
Cuts at: 2320 2678 2320
Size: 358 3691
BstAPI GCAn_nnn'nTGC
Cuts at: 1426 1426
Size: 4049
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstXI CCAn_nnnn'nTGG
Cuts at: 342 786 342
Size: 444 3605
BstYI r'GATC_y
Cuts at: 1 1074 1541 1564 1899 2145 3157 3243 3255
Size: 1073 467 23 335 246 1012 86 12
Cuts at: 3255 3601 1
Size: 346 449
Fragments arranged by size:
1073 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 177 335 451 561 785 107
Size: 70 158 116 110 224 3371
Fragments arranged by size:
3371 224 158 116 110 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 606 1999 2180 2343 606
Size: 1393 181 163 2312
Fragments arranged by size:
2312 1393 181 163
BtsI GCAGTG_nn'
Cuts at: 1292 1292
Size: 4049
DraI TTT'AAA
Cuts at: 1048 1048
Size: 4049
DrdI GACnn_nn'nnGTC
Cuts at: 1755 2613 1755
Size: 858 3191
EaeI y'GGCC_r
Cuts at: 344 528 612 1634 1808 2199 2226 3571 344
Size: 184 84 1022 174 391 27 1345 822
Fragments arranged by size:
1345 1022 822 391 184 174 84 27
EagI C'GGCC_G
Cuts at: 528 1634 528
Size: 1106 2943
EarI CTCTTCn'nnn_
Cuts at: 1007 2072 2282 1007
Size: 1065 210 2774
Fragments arranged by size:
2774 1065 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 535 2257 2567 2713 3859 127
Size: 408 1722 310 146 1146 317
Fragments arranged by size:
1722 1146 408 317 310 146
EcoICRI GAG'CTC
Cuts at: 762 1061 762
Size: 299 3750
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1874 2306 3053 3321 1874
Size: 432 747 268 2602
Fragments arranged by size:
2602 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 400 586 639 735 1188 400
Size: 186 53 96 453 3261
Fragments arranged by size:
3261 453 186 96 53
EcoRI G'AATT_C
Cuts at: 472 1053 472
Size: 581 3468
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 294 819 2068 294
Size: 525 1249 2275
Fragments arranged by size:
2275 1249 525
FspI TGC'GCA
Cuts at: 541 635 1830 541
Size: 94 1195 2760
Fragments arranged by size:
2760 1195 94
HaeII r_GCGC'y
Cuts at: 243 434 731 801 920 1483 1731 2753 3732
Size: 191 297 70 119 563 248 1022 979
Cuts at: 3732 243
Size: 560
Fragments arranged by size:
1022 979 563 560 297 248 191 119
70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1891 1921 1923 1953 1891
Size: 30 2 30 3987
Fragments arranged by size:
3987 30 30 2
HincII GTy'rAC
Cuts at: 558 1088 558
Size: 530 3519
HindIII A'AGCT_T
Cuts at: 973 973
Size: 4049
KasI G'GCGC_C
Cuts at: 430 727 916 1479 1727 430
Size: 297 189 563 248 2752
Fragments arranged by size:
2752 563 297 248 189
KpnI G_GTAC'C
Cuts at: 1069 1069
Size: 4049
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2720 2904 2720
Size: 184 3865
MscI TGG'CCA
Cuts at: 1810 1810
Size: 4049
MslI CAynn'nnrTG
Cuts at: 340 784 2038 2165 340
Size: 444 1254 127 2224
Fragments arranged by size:
2224 1254 444 127
MspA1I CmG'CkG
Cuts at: 125 179 396 420 453 563 1001 1160 1474
Size: 54 217 24 33 110 438 159 314
Cuts at: 1474 1834 2847 3092 125
Size: 360 1013 245 1082
Fragments arranged by size:
1082 1013 438 360 314 245 217 159
110 54 33 24
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 4049
NarI GG'CG_CC
Cuts at: 431 728 917 1480 1728 431
Size: 297 189 563 248 2752
Fragments arranged by size:
2752 563 297 248 189
NcoI C'CATG_G
Cuts at: 107 335 107
Size: 228 3821
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 4049
NotI GC'GGCC_GC
Cuts at: 528 528
Size: 4049
NspI r_CATG'y
Cuts at: 1102 1602 2509 1102
Size: 500 907 2642
Fragments arranged by size:
2642 907 500
PasI CC'CwG_GG
Cuts at: 739 739
Size: 4049
PciI A'CATG_T
Cuts at: 2505 2505
Size: 4049
PfoI T'CCnGG_A
Cuts at: 764 953 764
Size: 189 3860
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4017
PspOMI G'GGCC_C
Cuts at: 399 585 735 399
Size: 186 150 3713
Fragments arranged by size:
3713 186 150
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 481 513 481
Size: 32 4017
PstI C_TGCA'G
Cuts at: 1096 1781 1096
Size: 685 3364
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4049
PvuII CAG'CTG
Cuts at: 396 420 1474 1834 396
Size: 24 1054 360 2611
Fragments arranged by size:
2611 1054 360 24
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 764 1063 764
Size: 299 3750
SacII CC_GC'GG
Cuts at: 180 454 564 180
Size: 274 110 3665
Fragments arranged by size:
3665 274 110
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SapI GCTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 499 3566 499
Size: 3067 982
SfcI C'TryA_G
Cuts at: 33 1092 1777 2770 2961 33
Size: 1059 685 993 191 1121
Fragments arranged by size:
1121 1059 993 685 191
SfoI GGC'GCC
Cuts at: 432 729 918 1481 1729 432
Size: 297 189 563 248 2752
Fragments arranged by size:
2752 563 297 248 189
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 591 719 1071 591
Size: 128 352 3569
Fragments arranged by size:
3569 352 128
SmlI C'TyrA_G
Cuts at: 283 463 511 1204 2119 2611 2873 283
Size: 180 48 693 915 492 262 1459
Fragments arranged by size:
1459 915 693 492 262 180 48
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4049
SphI G_CATG'C
Cuts at: 1102 1602 1102
Size: 500 3549
StyI C'CwwG_G
Cuts at: 107 269 335 1183 1537 107
Size: 162 66 848 354 2619
Fragments arranged by size:
2619 848 354 162 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1632 1765 2224 2433 1632
Size: 133 459 209 3248
Fragments arranged by size:
3248 459 209 133
TatI w'GTAC_w
Cuts at: 497 3564 497
Size: 3067 982
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3566 3598 3566
Size: 32 4017
Tth111I GACn'n_nGTC
Cuts at: 1846 1846
Size: 4049
XbaI T'CTAG_A
Cuts at: 1080 1080
Size: 4049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1544 1544
Size: 4049
XmaI C'CCGG_G
Cuts at: 589 717 1069 589
Size: 128 352 3569
Fragments arranged by size:
3569 352 128
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PciI PfoI PmeI PpiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI BpmI
Bpu10I BsaXI BsgI BsiWI BsmI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NruI NsiI PacI PflMI
PmlI PpuMI PshAI PsiI PspXI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI