Restriction map for pF1KB9822
 (Linear) (Six-base) MAPSORT of: KIBB9822.seq  Check: 2764  from: 1  to: 936



KIBB9822.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AfeI AGC'GCT

Cuts at:      0     133     691     936 
   Size:        133     558     245

  Fragments arranged by size: 

                 558     245     133
 
AlwNI CAG_nnn'CTG

Cuts at:      0      99     936 
   Size:         99     837
 
ApaI G_GGCC'C

Cuts at:      0     295     481     631     936 
   Size:        295     186     150     305

  Fragments arranged by size: 

                 305     295     186     150
 
ApoI r'AATT_y

Cuts at:      0     364     936 
   Size:        364     572
 
AvaI C'yCGr_G

Cuts at:      0     304     481     609     936 
   Size:        304     177     128     327

  Fragments arranged by size: 

                 327     304     177     128
 
BanI G'GyrC_C

Cuts at:      0     322     619     664     808     936 
   Size:        322     297      45     144     128

  Fragments arranged by size: 

                 322     297     144     128      45
 
BanII G_rGCy'C

Cuts at:      0     295     481     631     656     936 
   Size:        295     186     150      25     280

  Fragments arranged by size: 

                 295     280     186     150      25
 
BbeI G_GCGC'C

Cuts at:      0     326     623     812     936 
   Size:        326     297     189     124

  Fragments arranged by size: 

                 326     297     189     124
 
BglI GCCn_nnn'nGGC

Cuts at:      0     614     635     936 
   Size:        614      21     301

  Fragments arranged by size: 

                 614     301      21
 
Bme1580I G_kGCm'C

Cuts at:      0     295     481     631     667     936 
   Size:        295     186     150      36     269

  Fragments arranged by size: 

                 295     269     186     150      36
 
BmgBI CAC'GTC

Cuts at:      0     880     936 
   Size:        880      56
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     120     684     936 
   Size:        120     564     252

  Fragments arranged by size: 

                 564     252     120
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     340     388     936 
   Size:        160     180      48     548

  Fragments arranged by size: 

                 548     180     160      48
 
BsaHI Gr'CG_yC

Cuts at:      0     323     620     809     936 
   Size:        323     297     189     127

  Fragments arranged by size: 

                 323     297     189     127
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     343     934     936 
   Size:        343     591       2

  Fragments arranged by size: 

                 591     343       2
 
BseYI C'CCAG_C

Cuts at:      0     258     288     936 
   Size:        258      30     648

  Fragments arranged by size: 

                 648     258      30
 
BsiEI CG_ry'CG

Cuts at:      0     423     936 
   Size:        423     513
 
BsiHKAI G_wGCw'C

Cuts at:      0     656     936 
   Size:        656     280
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      82     936 
   Size:         82     854
 
Bsp1286I G_dGCh'C

Cuts at:      0     295     481     631     656     667     936 
   Size:        295     186     150      25      11     269

  Fragments arranged by size: 

                 295     269     186     150      25      11
 
BsrBI CCG'CTC

Cuts at:      0     503     936 
   Size:        503     433
 
BsrDI GCAATG_nn'

Cuts at:      0     771     936 
   Size:        771     165
 
BsrFI r'CCGG_y

Cuts at:      0     818     936 
   Size:        818     118
 
BssHII G'CGCG_C

Cuts at:      0     348     350     936 
   Size:        348       2     586

  Fragments arranged by size: 

                 586     348       2
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     234     678     936 
   Size:        234     444     258

  Fragments arranged by size: 

                 444     258     234
 
BtgI C'CryG_G

Cuts at:      0      69     227     343     453     677     936 
   Size:         69     158     116     110     224     259

  Fragments arranged by size: 

                 259     224     158     116     110      69
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     498     936 
   Size:        498     438
 
EaeI y'GGCC_r

Cuts at:      0     236     420     504     936 
   Size:        236     184      84     432

  Fragments arranged by size: 

                 432     236     184      84
 
EagI C'GGCC_G

Cuts at:      0     420     936 
   Size:        420     516
 
EarI CTCTTCn'nnn_

Cuts at:      0     899     936 
   Size:        899      37
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     427     936 
   Size:         19     408     509

  Fragments arranged by size: 

                 509     408      19
 
EcoICRI GAG'CTC

Cuts at:      0     654     936 
   Size:        654     282
 
EcoO109I rG'GnC_Cy

Cuts at:      0     292     478     531     627     936 
   Size:        292     186      53      96     309

  Fragments arranged by size: 

                 309     292     186      96      53
 
EcoRI G'AATT_C

Cuts at:      0     364     936 
   Size:        364     572
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     186     711     936 
   Size:        186     525     225

  Fragments arranged by size: 

                 525     225     186
 
FspI TGC'GCA

Cuts at:      0     433     527     936 
   Size:        433      94     409

  Fragments arranged by size: 

                 433     409      94
 
HaeII r_GCGC'y

Cuts at:      0     135     326     623     693     812     936 
   Size:        135     191     297      70     119     124

  Fragments arranged by size: 

                 297     191     135     124     119      70
 
HincII GTy'rAC

Cuts at:      0     450     936 
   Size:        450     486
 
HindIII A'AGCT_T

Cuts at:      0     865     936 
   Size:        865      71
 
KasI G'GCGC_C

Cuts at:      0     322     619     808     936 
   Size:        322     297     189     128

  Fragments arranged by size: 

                 322     297     189     128
 
MslI CAynn'nnrTG

Cuts at:      0     232     676     936 
   Size:        232     444     260

  Fragments arranged by size: 

                 444     260     232
 
MspA1I CmG'CkG

Cuts at:      0      17      71     288     312     345     455     893     936 
   Size:         17      54     217      24      33     110     438      43

  Fragments arranged by size: 

                 438     217     110      54      43      33      24      17
            
 
NarI GG'CG_CC

Cuts at:      0     323     620     809     936 
   Size:        323     297     189     127

  Fragments arranged by size: 

                 323     297     189     127
 
NcoI C'CATG_G

Cuts at:      0     227     936 
   Size:        227     709
 
NotI GC'GGCC_GC

Cuts at:      0     420     936 
   Size:        420     516
 
PasI CC'CwG_GG

Cuts at:      0     631     936 
   Size:        631     305
 
PfoI T'CCnGG_A

Cuts at:      0     656     845     936 
   Size:        656     189      91

  Fragments arranged by size: 

                 656     189      91
 
PspOMI G'GGCC_C

Cuts at:      0     291     477     627     936 
   Size:        291     186     150     309

  Fragments arranged by size: 

                 309     291     186     150
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     373     405     936 
   Size:        373      32     531

  Fragments arranged by size: 

                 531     373      32
 
PvuII CAG'CTG

Cuts at:      0     288     312     936 
   Size:        288      24     624

  Fragments arranged by size: 

                 624     288      24
 
SacI G_AGCT'C

Cuts at:      0     656     936 
   Size:        656     280
 
SacII CC_GC'GG

Cuts at:      0      72     346     456     936 
   Size:         72     274     110     480

  Fragments arranged by size: 

                 480     274     110      72
 
ScaI AGT'ACT

Cuts at:      0     391     936 
   Size:        391     545
 
SfoI GGC'GCC

Cuts at:      0     324     621     810     936 
   Size:        324     297     189     126

  Fragments arranged by size: 

                 324     297     189     126
 
SmaI CCC'GGG

Cuts at:      0     483     611     936 
   Size:        483     128     325

  Fragments arranged by size: 

                 483     325     128
 
SmlI C'TyrA_G

Cuts at:      0     175     355     403     936 
   Size:        175     180      48     533

  Fragments arranged by size: 

                 533     180     175      48
 
StyI C'CwwG_G

Cuts at:      0     161     227     936 
   Size:        161      66     709

  Fragments arranged by size: 

                 709     161      66
 
TatI w'GTAC_w

Cuts at:      0     389     936 
   Size:        389     547
 
XmaI C'CCGG_G

Cuts at:      0     481     609     936 
   Size:        481     128     327

  Fragments arranged by size: 

                 481     327     128
 

 Enzymes that do cut:

     AfeI    AlwNI     ApaI     ApoI     AvaI     BanI    BanII     BbeI
     BglI Bme1580I    BmgBI     BplI    BpuEI    BsaHI    BseRI    BseYI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BsrBI    BsrDI    BsrFI   BssHII
    BstXI     BtgI    BtgZI     EaeI     EagI     EarI     EciI  EcoICRI
 EcoO109I    EcoRI     FalI     FspI    HaeII   HincII  HindIII     KasI
     MslI   MspA1I     NarI     NcoI     NotI     PasI     PfoI   PspOMI
     PsrI    PvuII     SacI    SacII     ScaI     SfoI     SmaI     SmlI
     StyI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI     BmrI     BmtI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI     BsgI    BsiWI     BsmI
    BspEI    BspHI    BspMI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI  Eco57MI    EcoNI    EcoRV     FseI    FspAI    Hin4I     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NdeI   NgoMIV
     NheI     NruI     NsiI     NspI     PacI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PstI     PvuI
    RsrII     SalI    SanDI     SapI     SbfI    SexAI     SfcI     SfiI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI
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